Blending fishwastes and chicken manure extract as low-cost and stable diet for mass culture of freshwater zooplankton, optimized for aquaculture

Abstract

This study investigated the feasibility of fishwastes and chicken manure extract (CME) as cheap diet for mass culture of freshwater zooplankton. CME and fishwastes as well as carbon source were used to make fishwaste diets (FWD). Each diet was triplicated 3 days before inoculation with 5, 2 and 0.4 ind ml−1 of rotifers, copepods and cladocerans, respectively in each tank. About 5ml of water was done daily, from which the zooplankters were counted. Harvesting was done at the first exponential growth phase by replacing 50% of the water and FWD. There was a significantly higher density of zooplankton and SGR in FWDB than FWDA and control tanks. The zooplankton obtained highest densities on day 7 as follows: rotifers: 100.6±14.8, 146.3±7.0, and 60.0±7.9 ind ml−1 in FWDA, FWDB and the control tanks, respectively; the copepods: 8.0±11.1, 12.6±13.6 and 4.3±2.1 ind ml−1 in FWDA, FWDB and control tanks, respectively; the cladocerans: 3.3±6.0, 8.6±8.7 and 3.6±2.5 ind ml−1 in FWDA, FWDB and control tanks, respectively. The most abundant genera were Brachionus sp., Cyclops sp. and Daphnia sp. for the rotifers, copepods and cladocerans, respectively. This study offers an alternative to expensive on-site microalgal production toward a more cost-effective aquaculture.

First record of a seed shrimp (Ostracoda: Podocopida) Cypretta campechensis (Cyprididae) in a perennial lake (Coimbatore, India): Its molecular identification

“…have been recorded; in addition to these, the presence of C. campechensis indicates the water was getting polluted during the summer. The mass culture of zooplankton, such as Brachionus, Daphnia, Asplanchna intermedia, Ceriodaphnia, Eucyclops speratus, Mesocyclops edax, Moina, Cyclops, Lecanea, Keratella, Diaphanosoma and Diaptomus with different feeds, such as chlorella, Yeast, cow-dung, mixed phytoplankton, condensed phytoplankton products, pulse bran water, poultry manure, snail faeces, chicken manure and fish waste diet have been reported (Bhavan et al, 2016;Kalpana et al, 2018;Ogello et al, 2019) [12,13,55] . In this study, the first reported zooplankton species C. campechensis have grown well in the laboratory with mixed phytoplankton diet.…”

Culturing live foods for fish larviculture using non‐microalgal diet: The role of waste‐generated bacteria and selected commercial probiotics—A review

“…Addition of chicken dropping to fishpond also leads to production of heterotrophic bacteria that can be utilized by rotifer and other zooplanktons as a diet (Jenkins et al, 2006). Ogello et al (2019) blended CME and FWD to culture zooplankton in outdoor culture tanks. In this study, there was significantly higher production of zooplankton in CME + FWD cultures (SGR of 0.42-0.62 day −1 ) than in control cultures (SGR of 0.35-0.48 day −1 ).…”

“…Rotifers have broad nutritional requirements that must be met to produce stable cultures. Ogello et al (2019) conducted a preliminary study on the dietary value of FWD-fed rotifer B. rotundiformis (SS-type) for larval rearing of marine fish larvae Silago japonica under laboratory conditions. Even though the study recorded a low survival rate of 9.7%, which is expected in most marine fish species, the value was comparable to the control experiment in which supplemented microalgae, C. vulgaris, was used to culture the rotifers (Ogello et al, 2019).…”

“…Since most marine fish larvae cannot synthesize DHA from precursor molecules, for example, EPA or α-linolenic acid (ALA), supplying DHA-rich feeds to fish larvae is important (Masuda et al, 1998). Ogello et al (2019) obtained DHA/ARA ratio of 2.4 in the waste-fed rotifers, which is a positive step towards culturing marine larval fishes without supplementation with expensive emulsions. However, the optimal DHA/ARA ratio required for proper larval fish development is 10.0 (Sargent et al, 1999).…”

Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae

“…The prices of these two non-living microalgal-based foods are expensive, therefore they are not preferred by hatcheries in developing countries (Wullur et al 2020). This led to the development of an inexpensive and easy diet as an alternative food for microalgae, the fish waste diet (FWD) (Ogello et al 2018;Napitupulu et al 2019;Ogello et al 2019Ogello et al , 2020Wullur et al 2019Wullur et al , 2020. The FWD was developed from the composition of fish waste and probiotic bacteria.…”