Blood cells separation microfluidic chip based on dielectrophoretic force

Abstract

The paper proposed a dielectrophoresis microfluidic chip for particle separation, which uses dielectric properties to perform size-based fractionation of blood cells. Using the principle of control variables, the value range of each parameter satisfying the separation condition was calculated by MATLAB and COMSOL software simulation and the specific parameters were selected to simulate the motion trajectory of cells in the microfluidic channel. At this time, all three kinds of particles were negative dielectrophoretic responses. The spheroid white blood cells were subjected to a stronger dielectrophoretic force than red blood cells and platelets, biased toward the right exit. Platelets and red blood cells flowed out from the left and middle outlets, respectively, under the combined action of fluid force and dielectrophoretic force to achieve the purpose of separation.

A simple and reversible glass–glass bonding method to construct a microfluidic device and its application for cell recovery

“…This method significantly improves both the productivity and the usability of glass microfluidic devices and extend the possibility of glass microfluidic applications in addition to the cell recovery system demonstrated in this study. For instance, with a miniaturized pump, 58 an on-chip valve 59 and a cell separator, 60,61 the present microfluidic system can be utilized as a portable analysis system which can be constructed to confine analytes on-site. Such systems should be user-friendly and useful for high throughput on-site biomedical or environmental analysis.…”

Two‐stage particle separation channel based on standing surface acoustic wave

“…There are mainly inertial force sorting, 12 hydrodynamic sorting, 13 micro-structure filtering and screening 14 and other technologies. The other is an active sorting method, which is to apply an external force field to make different suspended particles in the micro flow channel move along different trajectories to achieve the purpose of sorting, mainly including dielectrophoresis sorting, 15 sound force sorting, 16,17 fluorescence excitation sorting 18 and magnetic sorting, 19 etc. These methods other than acoustic sorting are limited in practical applications due to their damage to biological particles or restrictions on specific conditions.…”

Particle separation in microfluidics using different modal ultrasonic standing waves

“…Especially in the fight against COVID-19 this year, various detection methods and vaccine development technologies developed based on microfluidic technology have played a key role [11] , [12] . Microsomal chips can realize many functions including sorting [13] , [14] , mixing [15] , [16] , reaction [17] , cell culture [18] , etc, among which sorting is one of the most core functions. The sorting method using microfluidics can be divided into active method and passive method.…”