Blood coagulation and platelet adhesion on polyaniline films

Humpolíček, Petr; Kuceková, Zdenka; Kašpárková, Věra; Pelková, Jana; Modić, Martina; Junkar, Ita; Bober, Patrycja; Stejskal, Jaroslav; Lehocký, Marián

doi:10.1016/j.colsurfb.2015.06.008

Cited by 21 publications

(16 citation statements)

References 45 publications

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“…Moreover, not only the modification of the cell/PANI surface interaction is possible, but also the interaction with blood can be influenced. The anticoagulation effect of PANI reprotonated with PAMPSA via the interaction of film with coagulation factors X, V and II has previously been described [ 11 ]. Apart from PAMPSA, other acids may be utilized, e.g., sulfamic and phosphotungstic acid.…”

Section: Introductionmentioning

confidence: 99%

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Rejmontová

Capáková

Mikušová

et al. 2016

IJMS

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Polyaniline shows great potential and promises wide application in the biomedical field thanks to its intrinsic conductivity and material properties, which closely resemble natural tissues. Surface properties are crucial, as these predetermine any interaction with biological fluids, proteins and cells. An advantage of polyaniline is the simple modification of its surface, e.g., by using various dopant acids. An investigation was made into the adhesion, proliferation and migration of mouse embryonic fibroblasts on pristine polyaniline films and films doped with sulfamic and phosphotungstic acids. In addition, polyaniline films supplemented with poly (2-acrylamido-2-methyl-1-propanesulfonic) acid at various ratios were tested. Results showed that the NIH/3T3 cell line was able to adhere, proliferate and migrate on the pristine polyaniline films as well as those films doped with sulfamic and phosphotungstic acids; thus, utilization of said forms in biomedicine appears promising. Nevertheless, incorporating poly (2-acrylamido-2-methyl-1-propanesulfonic) acid altered the surface properties of the polyaniline films and significantly affected cell behavior. In order to reveal the crucial factor influencing the surface/cell interaction, cell behavior is discussed in the context of the surface energy of individual samples. It was clearly demonstrated that the lesser the difference between the surface energy of the sample and cell, the more cyto-compatible the surface is.

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Section: Introductionmentioning

confidence: 99%

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Rejmontová

Capáková

Mikušová

et al. 2016

IJMS

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“…Humpolíček et al 18 revealed that PANI-PAMPSA possesses signicant impact on blood coagulation and platelet adhesion. As regards conductivity, a value of about 10 À2 S cm À1 was reported for PANI-PAMPSA,…”

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confidence: 99%

Stem cell differentiation on conducting polyaniline

et al. 2015

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Polyaniline is a promising conducting polymer with broad application potential in biomedicine. Its medical use, however, requires both biocompatibility and suitable physico-chemical and surface properties. The microstructure, electrical properties, and surface characteristics of polyaniline salt, polyaniline base, and polyaniline deposited with biologically active poly(2-acrylamido-2-methyl-1-propanesulfonic acid) were revealed using atomic force microscopy, contact angle measurements, and Raman spectroscopy. As conducting polymers can be preferentially applied in tissue engineering of heart and nervous tissues, the cardiomyogenesis in pure cardiomyocytes derived from embryonic stem cells and neurogenesis in neural progenitors isolated from embryonal 13 dpc brain were further investigated. The results show that neither cardiomyogenesis nor neurogenesis were influenced by any of the tested polyaniline films.However, the most favorable cell behaviour was observed on pristine polyaniline base; therefore, polyaniline in pristine forms without any further modification can be applied in a variety of biomedical fields.

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“…The blood coagulation cascade consists of the tissue-mediated extrinsic pathway, surface-mediated intrinsic pathway, and common coagulation pathway [ 42 ]. The examination of those coagulation pathways were carried out for all samples by means of prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT).…”

Section: Resultsmentioning

confidence: 99%

“…TT is a measure of thrombin formation time by converting fibrinogen to fibrin in the common coagulation pathway [ 42 ]. The lower limit for TT to perform anticoagulation activity is 20 s [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

A New Route of Fucoidan Immobilization on Low Density Polyethylene and Its Blood Compatibility and Anticoagulation Activity

Özaltın

Lehocký

Humpolíček

et al. 2016

IJMS

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Beside biomaterials’ bulk properties, their surface properties are equally important to control interfacial biocompatibility. However, due to the inadequate interaction with tissue, they may cause foreign body reaction. Moreover, surface induced thrombosis can occur when biomaterials are used for blood containing applications. Surface modification of the biomaterials can bring enhanced surface properties in biomedical applications. Sulfated polysaccharide coatings can be used to avoid surface induced thrombosis which may cause vascular occlusion (blocking the blood flow by blood clot), which results in serious health problems. Naturally occurring heparin is one of the sulfated polysaccharides most commonly used as an anticoagulant, but its long term usage causes hemorrhage. Marine sourced sulfated polysaccharide fucoidan is an alternative anticoagulant without the hemorrhage drawback. Heparin and fucoidan immobilization onto a low density polyethylene surface after functionalization by plasma has been studied. Surface energy was demonstrated by water contact angle test and chemical characterizations were carried out by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Surface morphology was monitored by scanning electron microscope and atomic force microscope. Finally, their anticoagulation activity was examined for prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT).

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Blood coagulation and platelet adhesion on polyaniline films

Cited by 21 publications

References 45 publications

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Stem cell differentiation on conducting polyaniline

A New Route of Fucoidan Immobilization on Low Density Polyethylene and Its Blood Compatibility and Anticoagulation Activity

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