Bonding of cartilage matrices with cultured chondrocytes: An experimental model

Peretti, Giuseppe M.; Randolph, Mark A.; Caruso, Enzo M.; Rossetti, Francesco; Zaleske, David J.

doi:10.1002/jor.1100160115

Cited by 71 publications

(73 citation statements)

References 24 publications

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“…To evaluate bovine meniscal healing, we translated the CS-BM adhesive to a subcutaneous implantation model, one with which our laboratory has extensive experience [45][46][47][48] and which has been used similarly in meniscal repair studies. [49][50][51] While not in the joint space, this small animal model allows us to simply and economically simulate more closely a natural environment for meniscus fusion so that we may more accurately evaluate biomaterial function and meniscus repair over time. This subcutaneous meniscus repair model allows us to evaluate the repair potential of meniscus tissue without a blood supply, which is relevant for comparisons to the white zone of meniscus.…”

Section: Fig 3 Biochemical Assays and Immunohistochemistry Of Encapmentioning

confidence: 99%

Bonding and Fusion of Meniscus Fibrocartilage Using a Novel Chondroitin Sulfate Bone Marrow Tissue Adhesive

Simson

Strehin

Allen

et al. 2013

Tissue Engineering Part A

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The weak intrinsic meniscus healing response and technical challenges associated with meniscus repair contribute to a high rate of repair failures and meniscectomies. Given this limited healing response, the development of biologically active adjuncts to meniscal repair may hold the key to improving meniscal repair success rates. This study demonstrates the development of a bone marrow (BM) adhesive that binds, stabilizes, and stimulates fusion at the interface of meniscus tissues. Hydrogels containing several chondroitin sulfate (CS) adhesive levels (30, 50, and 70 mg/mL) and BM levels (30%, 50%, and 70%) were formed to investigate the effects of these components on hydrogel mechanics, bovine meniscal fibrochondrocyte viability, proliferation, matrix production, and migration ability in vitro. The BM content positively and significantly affected fibrochondrocyte viability, proliferation, and migration, while the CS content positively and significantly affected adhesive strength (ranged from 60 -17 kPa to 335 -88 kPa) and matrix production. Selected material formulations were translated to a subcutaneous model of meniscal fusion using adhered bovine meniscus explants implanted in athymic rats and evaluated over a 3-month time course. Fusion of adhered meniscus occurred in only the material containing the highest BM content. The technology can serve to mechanically stabilize the tissue repair interface and stimulate tissue regeneration across the injury site.

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Section: Fig 3 Biochemical Assays and Immunohistochemistry Of Encapmentioning

confidence: 99%

Bonding and Fusion of Meniscus Fibrocartilage Using a Novel Chondroitin Sulfate Bone Marrow Tissue Adhesive

Simson

Strehin

Allen

et al. 2013

Tissue Engineering Part A

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“…26,27 However, a more recent report proposed that proteoglycans in cartilage prevent cells from adhering to a defect. Improvement of adherence of added cells might be achieved by applying enzymes that cleave matrix components such as GAGs.…”

Section: Disscussionmentioning

confidence: 99%

Injecting partially digested cartilage fragments into a biphasic scaffold to generate osteochondral composites in a nude mice model

Liao

Lin

Chiang

et al. 2006

J Biomedical Materials Res

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This study proposed a novel scaffold with heterogeneous morphology that mimics the natural tissue. Its upper part contains a hollow cavity surrounded by a wall of poly(L-lactic-co-glycolic acid) (PLGA) porous membrane for injecting cartilage tissue and cells. An interconnecting porous structure located under the hollow cavity was made of composite materials that combined PLGA and beta-tricalcium phosphate (beta-TCP) to simulate the subchondral bone. Adult pig articular cartilage was cut and sieved into small fragments. The tissue fragments was partially digested by 0.1% collagenase for 0, 2, 4, and 6 h and injected into the hollow cavity of the biphasic scaffold. The biphasic scaffolds were then implanted into the subcutaneous pocket of nude mice for 4 weeks. No tissue bonding or new cartilaginous tissue formation was identified in the cartilage fragment without enzymatic treatment. The cartilage fragments digested with 2 h of collagenase digestion were partially integrated after implantation. The integrative properties of the cartilage fragment depended on the extent of enzymatic digestion. Releasing cells at the tissue surface enhanced confluence and bonding of the cartilage fragment matrix. Complete integration of the cartilage fragments and cartilage remodeling were achieved by digestion of the tissue fragments with 4 h of enzymatic treatment. The neocartilage grew from the upper hollow cavity into the lower PLGA/beta-TCP porous structure, forming an interface similar to that formed between cartilage and subchondral bone. This study combined the osteochondral scaffold and limited cartilage tissues to generate cartilage tissue in vivo intending for repairing full-thickness articular cartilage defects.

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“…There are various scaffolds or vehicles that have been used for chondrocytes [Glowacki, 2000]. Devitalized cartilage matrix itself was used with fibrin as a substrate for neochondrogenesis by isolated chondrocytes [Peretti et al, 1998]. Collagen [Mizuno and Glowacki, 1996] and other natural polymers can be made as porous 3-D carriers for chondrocytes ( fig.…”

Section: Cartilagementioning

confidence: 99%

“…collagen) [Mizuno and Glowacki, 1996] or with biological glues as adjuncts (e.g. fibrin) [Peretti et al, 1998]. Tissue or organ transfers also need to be united with host vasculature and adequately revascularized throughout.…”

Section: Challengesmentioning

confidence: 99%

Engineered Cartilage, Bone, Joints, and Menisci

Glowacki¹

2001

Cells Tissues Organs

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Reconstruction of many musculoskeletal structures can be accomplished by bone grafting and implantation of prostheses. Alternate approaches are needed, however, for repair of complex structures such as articular cartilage surfaces and the temporomandibular meniscus and joint. Tissue engineering, either cell-free or cell-based, offers promise because of recent advances in materials research and in our knowledge of the cellular and molecular mechanisms of tissue repair. There are three considerations in designing a construct for engineered tissue: the source of cells, if any; the nature of the carrier or scaffold; and use, if any, of genes, factors, or adjuvants. Autogenous cells, often expanded in vitro, have been useful for cartilage tissue engineering. Precursor/progenitor cells are advantageous for bone tissue. There are many natural and synthetic resorbable materials with good biocompatibility and tissue compatibility that can be modified to have the porosity and mechanical properties needed for specific applications. The scaffolds can also be modified to provide biological signals to augment repair and integration.

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Bonding of cartilage matrices with cultured chondrocytes: An experimental model

Cited by 71 publications

References 24 publications

Bonding and Fusion of Meniscus Fibrocartilage Using a Novel Chondroitin Sulfate Bone Marrow Tissue Adhesive

Bonding and Fusion of Meniscus Fibrocartilage Using a Novel Chondroitin Sulfate Bone Marrow Tissue Adhesive

Injecting partially digested cartilage fragments into a biphasic scaffold to generate osteochondral composites in a nude mice model

Engineered Cartilage, Bone, Joints, and Menisci

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