Bovine serum albumin-imprinted polyacrylamide gel beads prepared via inverse-phase seed suspension polymerization

Bovine serum albumin-imprinted polymer gels prepared by graft copolymerization of acrylamide on chitosan

“…However, previous studies on protein-imprinted polymers indicate that most of the MIPs exhibited a much lower binding capacity for the target molecules (several milligram protein per gram wet MIP gels), despite a good binding specificity. In the studies by Hjerten et al [8][9][10], PAM gels imprinted with hemoglobin were reported to show high binding specificity, whereas the binding capacity was estimated to be less than 3 mg/g wet gels, which was in the agreement with the findings of Pang et al [24]. Recently, Guo et al [16,17] prepared hemoglobin-imprinted polymers by physically entrapping or chemically grafting of selective polyacrylamide (PAM) gels onto porous chitosan beads, and the MIPs obtained showed significantly enhanced template rebinding capacity.…”

Hemoglobin recognition of molecularly imprinted hydrogels prepared at different pHs

“…Previous studies have been reported on MIHs with protein with varying chemical structures or techniques, these studies have been described mainly the MIHs adsorption capacity, their imprinting effect and the mechanism of recognition [6][7][8][9][10]. However, selectivity of imprinted hydrogels has not been demonstrated in many systems.…”

Characteristic and Synthetic Approach of Molecularly Imprinted Polymer

“…Bovine serum albumin-imprinted polyacrylamide gel beads were synthesized via inverse-phase seed suspension polymerization, using high-density crosslinked gel beads as core, low-density crosslinked polyacrylamide gel as imprinting shell [67]. The selectivity test showed that imprinting gel beads exhibited good recognition for template proteins, as compared to the control protein.…”