C-Phycocyanin, a selective cyclooxygenase-2 inhibitor, induces apoptosis in lipopolysaccharide-stimulated RAW 264.7 macrophages

Reddy, Madhava C.; Jagu, Subhashini; Mahipal, Suraneni V.K.; Bhat, Vadiraja B.; Reddy, P. N.; Kiranmai, Gaddam; Madyastha, K. M.; Reddanna, Pallu

doi:10.1016/s0006-291x(03)00586-2

Cited by 180 publications

(97 citation statements)

References 36 publications

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“…These polar substances were found in extracts of different red, brown and green seaweeds (Matsukawa et al, 1997;Anggediredja et al, 1997;Lim et al, 2002;Santoso et al, 2004;Zhang et al, 2007;Shanab, 2007;Shalaby, 2008) as well as in micro algae and cyanobacterial species Reddy et al, 2003;Benedetti et al, 2004;Shalaby, 2004) these substances mainly include phycocyanin pigment, sulphated polysaccharides and phenolic compounds which are largely present in most macro, micro and cyanobacterial species which exhibited pronounced antioxidant activity. Non-polar antioxidant substances were recorded also in microalgae as Chlorella vulgaris, Dunaliella salina (Nirupama, 2004;Murthy et al, 2005;Zhang-Bao et al, 2004) in cyanobacteria (Shalaby, 2004) in addition to the different seaweed species (Krinsky, 1989;Hyun et al, 2003;Xi et al, 2003;Sook et al, 2004;Yuan et al, 2005;Shanab, 2007;Shalaby, 2008).…”

Section: Nacl (M)mentioning

confidence: 99%

Salt stress enhancement of antioxidant and antiviral efficiency of Spirulina platensis

Shalaby¹,

Shanab²,

Singh³

2010

J. Med. Plants Res.

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Cultivation of Spirulina platensis under salt stress conditions (0.02 M as control), 0.04 and 0.08 M NaCl led to a remarkable alteration of algal metabolism as well as an enhancement or induction of biologically active compounds. Concerning algal growth, salt stress caused a decrease in dry weight, chlorophyll a content as well as certain xanthophylls (neoxanthin and violaxanthin) while β-carotene production was stimulated especially at higher salt concentrations. Biochemical analysis of salt stressed algal revealed that lipid content was slightly increased together with certain saturated and unsaturated fatty acids especially the polyunsaturated ones (γ-inolenic acid, omega 3 fatty acid). Electrophoretic analysis of soluble protein pointed out that certain high molecular weight protein bands were not detected comparing with the protein marker. Five new protein bands of molecular weights 190, 158, 113, 77 and 28 KDa were recorded, in addition to an increase in the intensity of 6 already existing bands. Phosphate buffer and water extracts of the algal exhibited antiviral activities against both Hepatitis-A-virus-type-MBB (HAV-MBB strain, RNA virus) and Herpes simplex-virus-type-1 (HSV-1, DNA virus). Water extracts were found to be more effective than phosphate buffer extracts in inducing antiviral activities (98%) especially against HSV-1 virus. The same water extract of the salt stressed algal demonstrated higher anticoagulating activity compared with those of heparin and the positive control measured by clotting time assay. Antioxidant activity of the algal successive extracts against 2, 2 diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (ethylbenzthiazoline-6-sulfonic acid) radical methods revealed moderate antioxidant activity of the non-polar algal extracts (petroleum ether) which were doubled with increasing extract concentration. The lowest activity was recorded by the partially polar (ethyl acetate) algal extract of both concentrations at all salinity levels. While the polar extracts (ethanol and water) showed higher antioxidant activities which were doubled with increasing extract concentration. Ethanolic algal extract (100 µg/ ml at 0.08 M NaCl) exhibited the highest antioxidant activity compared with those of the synthetic antioxidant butylated hydroxy anisol as standard (85.0, 89.9 and 86.0, 91.8% respectively).Key words: Spirulina platensis, antioxidant-antivirus, biochemical studies, salt stress. INTRODUCTIONMany cyanobacteria and micro algae were considered as a natural source of various biologically and pharmacologically active compounds with structurally complex molecules which are difficult or impossible to be *Corresponding author. E-mail: dremad2009@yahoo.com.produced by chemical synthesis (Smith and Doan, 1999). Genus Spirulina has gained an importance and international demand for its high phytonutrients value and pigments which have applications in healthy foods, animal feed, therapeutics and diagnostics (Becker, 1994;Vonshak and Tomaselli, 2000). Spirulina has been used as food and nutritional ...

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Section: Nacl (M)mentioning

confidence: 99%

Salt stress enhancement of antioxidant and antiviral efficiency of Spirulina platensis

Shalaby¹,

Shanab²,

Singh³

2010

J. Med. Plants Res.

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“…Wound healing: These last years, researchers speculate that the minerals, phycobiliproteins, vitamins, beta-carotene, fatty acids, polysaccharides, phenolic compounds and volatile compounds present in these microalgae may accelerate wound healing by acting as an antioxidant and scavenging destructive free radicals responsible for cell death (Qishen et al, 1988;Kim et al, 1998;Hirahashi et al, 2002;Reddy et al, 2003;Subhashini et al, 2004;Li et al, 2005;Patel et al, 2005;Singh et al, 2005;El-Baky et al, 2009;Plaza et al, 2009). These reports confirmed that some unidentified factors in the crude extract might have enhanced the healing process.…”

Section: Mode Of Administrationmentioning

confidence: 99%

Review of twelve West Africa medicinal plants: Active phytochemical combinations in direct biochemically wound healing process

Edefia¹,

Tété‐Bénissan²,

Awaga³

et al. 2015

J. Med. Plants Res.

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“…The high content of proteins, essential amino acids, vitamins, minerals and fatty acids makes it an ideal healthy food (Ciferri, 1983). It is worthwhile to mention that this alga is rich in cphycocyanin, and the content can be up to 10~15% (Zhang, 1998), which has drawn attention, not only for the contribution to photosynthesis and photosynthetic evolution research (Wang et al 1996), but also for the great potentials in pharmaceutical usage (Bhat and Madyastha, 2000;Padyana et al 2001;Wang et al 2001;Reddy et al 2003;Subhashini et al 2004). C-phycocyanin consists of α-and β-subunits encoded by cpcA and cpcB gene, respectively.…”

Section: * Corresponding Authormentioning

confidence: 99%

The upstream sequence of the phycocyanin β subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity

Zhang¹,

Lu²

2005

Electro Journal of Biotech

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In cyanobacteria, few details are known of the mechanisms through which the expression of the lightharvesting pigment c-phycocyanin is regulated. In the present study, a 419 bp upstream sequence of the phycocyanin β subunit (cpcB) gene from Arthrospira platensis FACHB341 was fused with green fluorescent protein (gfp) gene, and a heterologous reporting system was built up to investigate the influence of light intensity on the expression of gfp gene, and the regulation function of different region of the upstream sequence of cpcB gene. Results showed that the upstream sequence of cpcB gene could drive the expression of gfp gene in Synechococcus sp. strain PCC7942, and the expression was influenced by light intensity, the lower the light intensity, the higher the GFP level. Deletion analysis revealed that a light-responsive element was located in the region -276 to-218, a promoter sequence was in the region -85 to -1, and two positive cis elements were in the -419 to -276 and the -218 to -130 regions, respectively.

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C-Phycocyanin, a selective cyclooxygenase-2 inhibitor, induces apoptosis in lipopolysaccharide-stimulated RAW 264.7 macrophages

Cited by 180 publications

References 36 publications

Salt stress enhancement of antioxidant and antiviral efficiency of Spirulina platensis

Salt stress enhancement of antioxidant and antiviral efficiency of Spirulina platensis

Review of twelve West Africa medicinal plants: Active phytochemical combinations in direct biochemically wound healing process

The upstream sequence of the phycocyanin β subunit gene from Arthrospira platensis regulates expression of gfp gene in response to light intensity

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