C-X-C chemokine receptor type 4 (CXCR4) is a key receptor for chicken primordial germ cell migration

Lee, Jeong Hyo; Park, Jeong-Woong; Kim, Si Won; Park, Joonghoon; Park, Tae Sub

doi:10.1262/jrd.2017-067

Cited by 13 publications

(9 citation statements)

References 28 publications

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“…Another report showed that the migratory capacity of CXCR4 knockout PGCs was significantly reduced in vivo after transplantation into embryos, and proved that CXCR4 was necessary for chicken PGCs to migrate into the gonad. However, they meanwhile found that CXCR4‐expressing somatic cell lines failed to migrate into the developing gonads, suggesting that another key factor(s) is necessary for directing PGCs into the genital ridges (Lee et al, 2017). For many years, researchers believed that CXCR4 was the only receptor for CXCL12, which regulates the trafficking of normal and malignant cells.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional dynamics of the circulating chicken primordial germ cells revealing key genes in cell adhesion and proliferation prior to gonad colonization

Huang

Lü

Wang

et al. 2022

Molecular Reproduction Devel

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Primordial germ cells (PGCs), precursors to sperms and oocytes, are responsible for the transfer of genetic information to the next generation. The PGCs arise far away from the developing gonad and thus have to migrate across the embryo to reach their site of function. The migration of PGCs from extraembryonic regions to the genital ridges is accomplished through distinct routes among different species. In particular, the birds PGCs utilized the developing circulation system to travel long distance before settling within the gonad. This study screened the transcriptome profile of chicken PGCs isolated from the bloodstream and the genital ridges to identify the cell intrinsic signals that could guide the unique migration path through circulation. We found cell adhesion and extracellular matrix (ECM) associated pathways were highly enriched in the PGCs from blood but not gonads. The platelet‐derived growth factor receptors (PDGFRA and PDGFRB) were downregulated during gonad colonization and knockdown of either PDGFRA or PDGFRB inhibit the proliferation of blood PGCs. Furthermore, the migration of blood PGCs was impaired by the suppression of PDGFRA but not PDGFRB. Hence, the chicken PGCs show dynamic transcriptional remodeling during the blood‐to‐gonad migration and colonization. The free‐floating PGCs in the circulation already express genes associated with cell–cell and cell‐ECM interactions and therefore prepare for gonadal colonization.

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Section: Discussionmentioning

confidence: 99%

Transcriptional dynamics of the circulating chicken primordial germ cells revealing key genes in cell adhesion and proliferation prior to gonad colonization

Huang

Lü

Wang

et al. 2022

Molecular Reproduction Devel

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“…For example, the generation and proliferation of PGCs are regulated by factors such as BMP4 and NANOS2 ( Lawson et al, 1999 ; Saga, 2010 ; Shirazi et al, 2012 ; Oulhen et al, 2019 ). Specifically, CVH and CXCR4 can be used to trace the migration process of PGCs ( Noce et al, 2001 ; Lee et al, 2017 ). Moreover, some genes like Prdm14 and Blimp1 are also considered to be crucial factors in PGC formation and differentiation ( Ohinata et al, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

C1EIP Functions as an Activator of ENO1 to Promote Chicken PGCs Formation via Inhibition of the Notch Signaling Pathway

et al. 2020

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The production of germ cells, especially primordial germ cells (PGCs), is important for avian stem cells and reproduction biology. However, key factors involved in the regulation of PGCs remain unknown. Here, we report a PGC-related marker gene: C1EIP (Chromosome 1 Expression in PGCs), whose activation and expression are regulated by the transcription factor STAT3 (signal transducer and activator of transcription 3), histone acetylation, and promoter methylation. C1EIP regulates PGCs formation by mediating the expression of PGC-associated genes, such as CVH (Chicken Vasa Homologous) and CKIT (Chicken KIT proto-oncogene). C1EIP knockdown during embryonic development reduces PGC generation efficiency both in vitro and in ovo . Conversely, C1EIP overexpression increases the formation efficiency of PGCs. C1EIP encodes a cytoplasmic protein that interacts with ENO1 (Enolase 1) in the cytoplasm, inhibits the Notch signaling pathway, and positively regulates PGC generation. Collectively, our findings demonstrate C1EIP as a novel gene involved in PGC formation, which regulates genes involved in embryonic stem cell differentiation through interaction with ENO1 and subsequent inhibition of the Notch signaling pathway by the impression of Myc (MYC proto-oncogene).

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“…Lee et al (2017a) were able to induce acquired resistance to ALV-J infection by using the CRISPR/Cas9-mediated homologous recombination in cultured chicken DF-1 cells. Lee et al (2017c) modified critical residues of chicken NHE1 in cultured cells to induce resistance to viral infection and create mutations of the tryptophan residue at position 38 (Trp38) using singlestranded oligodeoxynucleotide (ssODN) recombination to confer resistance to ALV-J. In another research by Koslová et al (2018), genetic resistance to ALV was successfully induced using the CRISPR/Cas9-mediated approach.…”

Section: Agricultural Applications Of Crispr/cas9 System In Poultrymentioning

confidence: 99%

Recent Advances in the Application of CRISPR/Cas9 Gene Editing System in Poultry Species

Khwatenge

Nahashon

2021

Front. Genet.

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CRISPR/Cas9 system genome editing is revolutionizing genetics research in a wide spectrum of animal models in the genetic era. Among these animals, is the poultry species. CRISPR technology is the newest and most advanced gene-editing tool that allows researchers to modify and alter gene functions for transcriptional regulation, gene targeting, epigenetic modification, gene therapy, and drug delivery in the animal genome. The applicability of the CRISPR/Cas9 system in gene editing and modification of genomes in the avian species is still emerging. Up to date, substantial progress in using CRISPR/Cas9 technology has been made in only two poultry species (chicken and quail), with chicken taking the lead. There have been major recent advances in the modification of the avian genome through their germ cell lineages. In the poultry industry, breeders and producers can utilize CRISPR-mediated approaches to enhance the many required genetic variations towards the poultry population that are absent in a given poultry flock. Thus, CRISPR allows the benefit of accessing genetic characteristics that cannot otherwise be used for poultry production. Therefore CRISPR/Cas9 becomes a very powerful and robust tool for editing genes that allow for the introduction or regulation of genetic information in poultry genomes. However, the CRISPR/Cas9 technology has several limitations that need to be addressed to enhance its use in the poultry industry. This review evaluates and provides a summary of recent advances in applying CRISPR/Cas9 gene editing technology in poultry research and explores its potential use in advancing poultry breeding and production with a major focus on chicken and quail. This could aid future advancements in the use of CRISPR technology to improve poultry production.

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C-X-C chemokine receptor type 4 (CXCR4) is a key receptor for chicken primordial germ cell migration

Cited by 13 publications

References 28 publications

Transcriptional dynamics of the circulating chicken primordial germ cells revealing key genes in cell adhesion and proliferation prior to gonad colonization

Transcriptional dynamics of the circulating chicken primordial germ cells revealing key genes in cell adhesion and proliferation prior to gonad colonization

C1EIP Functions as an Activator of ENO1 to Promote Chicken PGCs Formation via Inhibition of the Notch Signaling Pathway

Recent Advances in the Application of CRISPR/Cas9 Gene Editing System in Poultry Species

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