Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture

Abstract

Phoneix dactylifera L. commonly called date palm is a highly valuable horticultural cash crop for arid and semi-arid regions. The availability of offshoots and their survival during plantation are major concern. Being dioecious tree, seed propagation in date palm do not produce true-to-type offspring and tissue culture propagation is the only viable option to supply quality-planting propagules. Hereby, we report callus culture and plantlet regeneration in female date palm using in vitro-derived adventitious shoot bud tissues as explants. Explants (89.33 ± 2.67%) produced callus culture on 0.8% agargelled Murashige and Skoog's basal medium containing 100.0 mg l -1 each polyvinylpyrrolidone, ascorbic acid and glutamine, 50.0 mg l -1 each citric acid, adenine sulphate and L-arginine as additives, 0.1% activated charcoal (AC), 100 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 3.0 mg l -1 2-isopentenyladenine (2-iP). Callus culture were amplified on medium containing 3.0 mg l -1 2-iP along with 50 mg l -1 2,4-D for 2 passages and 10 mg l -1 2,4-D for 2 passages. Cultures grew moderately, organized and subsequently regenerated into shoot bud like structures during gradual transfer from medium containing higher concentration of 2,4-D to lower concentration. Plantlets were developed by sub-culturing of differentiated buds on (1) hormone free medium supplied with 10.0% sucrose and (2) medium containing 100.0 mg l -1 each ascorbic acid and glutamine, 50.0 mg l -1 each citric acid, adenine sulphate and L-arginine as additives, 1.0 mg l -1 each 6-benzylaminopurine, kinetin, 2-iP and a-naphthaleneacetic acid. Plantlets were developed on medium containing 0.1% AC, 1.0 mg l -1 each indole-3-acetic acid and indole-3-butyric acid. Rooted plantlets were soil-transplanted and acclimatized through gradual exposure from in vitro to in vivo conditions. Simple adoption, higher culture regeneration and simultaneous production of rooted plantlets in a cyclic manner render the protocol useful for mass scale propagation of elite genotype of female date palm.

High Efficiency Regeneration System from Blueberry Leaves and Stems

“…The study showed that 2.0 mg/L CPPU and 0.2 mg/L 2-ip can promote callus induction, and 3.0 mg/LCPPU can promote the growth of embryogenic callus in blueberry leaves ( Figure 1 and Figure 2 ; Table 9 and Table 10 ). In addition, CPPU [ 36 , 37 , 38 ] and 2iP [ 39 , 40 , 41 ] are active and promote bud growth in blueberries. In the present experiment, 1.0 mg/LCPPU and 0.4 mg/L 2-ip promote bud production and growth in blueberry, and the growth state of buds is better ( Figure 3 ; Table 11 ).…”

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

“…Auxins are the best PGRs to enhance chances of callus formation [ 29 , 30 ]. Among the different auxins used, picloram and 2,4-D were the auxins that were successfully induced callus from leaf and midrib explants of P. bullata followed by NAA.…”

Screening of Anti-Peptic Ulcer Activity of Jasminum Sambac

“…In vitro derived callus formation maximum observed in 2,4-D similarely in combination of 2,4-D only shown high callus formation in Jasminum sambac. Similar hormone combination has shown higher activity in Jasminum grandiflorum plant [9,18]. The causes of gastric ulcer are whispered due to stress triggered the addition of hydrochloric acid secretion and/or stasis, and the rate discharge is also an important reason in the ulcer configuration due to the exposure of the stomach's undefended lumen to the accumulating acid [19].…”