Cancer Chemopreventive effect of Rodent Tuber (Typhonium flagelliforme (Lood) Bl) against DMBA-Induced Rats Breast Tumor

Maysarah, Hilda; Faradilla, Meutia; Sari, Irma; Illian, Didi Nurhadi

doi:10.5958/0974-360x.2020.01013.6

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LDE was to choose as a better extract than ethyl acetate and n-hexane. The IC50 of LDE was categorized in good range and able to try in next test like the ability to inhibit cell cycle, inducing apoptosis, and affect gene expression of PI3K/Akt [27].…”

Section: Cytotoxic Activity Of Duku Leaves Extractsmentioning

confidence: 99%

Duku (Lansium domesticum) Leaves Extract Induces Cell Cycle Arrest and Apoptosis of HepG2 Cells via PI3K/Akt Pathways

Lubis

Hasibuan²,

Syahputra³

et al. 2022

Trends Sci

View full text Add to dashboard Cite

Liver cancer is a type of malignant cancer that causes many deaths in the world. Efforts to find natural ingredients that can be used to treat liver cancer are very important. This study investigated the anticancer activity of ethanol extract of Duku (Lansium domesticum) leaves (LDE) through the cytotoxic activity, cell cycle inhibition mechanism and apoptosis influence through the PI3K/Akt pathways. Secondary metabolites of LDE were determined by common qualitative method. Cell cycle arrest was carried out by flow cytometer at inhibitory concentration 50 (IC50), while the apoptosis test was obtained using double staining method. The IC50 value was used to identifying the expression of PI3K/Akt genes. The analyzed results were proven LDE can induce HepG2 cell death which IC50 value of 19.93 ± 0.93 μg/mL (ethanol extract), 267.06 ± 3.24 μg/mL (ethyl acetate extract), and 216.47 ± 2.87 μg/mL (n-hexane extract). Cell cycle arrest activity in HepG2 cells which IC50 value of ethanol extract of LDE occurs in S phase. Observation of apoptosis occurrence was obtained getting apoptosis in cell line. PI3K/Akt genes expression of ethanol extract of LDE was obtained using RT-PCR method. The bands density of PI3K/Akt at IC50 value treatment of 0.55 ± 0.02; 0.25 ± 0.01. The LDE was determined to contain secondary metabolites which can inhibit the growth of HepG2 cells through PI3K/Akt pathways. HIGHLIGHTS The ethanolic extract of Lansium domesticum leaves have the best cytotoxic activity against HepG2 cells line compared to ethyl acetate extract and n-hexane extract The ethanolic extract of Lansium domesticum leaves have anticancer activity against HepG2 cells line through cell cycle arrest and apoptosis induce The ethanolic extract of Lansium domesticum leaves can inhibit the expressions of PI3K/Akt/mTOR genes using RT-PCR GRAPHICAL ABSTRACT

show abstract

Section: Cytotoxic Activity Of Duku Leaves Extractsmentioning

confidence: 99%

Duku (Lansium domesticum) Leaves Extract Induces Cell Cycle Arrest and Apoptosis of HepG2 Cells via PI3K/Akt Pathways

Lubis

Hasibuan²,

Syahputra³

et al. 2022

Trends Sci

View full text Add to dashboard Cite

show abstract

Preclinical anticancer activity of Typhonium flagelliforme (Lodd.) Blume and its potential mechanism: A systematic review

Ng,

Tan,

Looi

et al. 2023

Journal of Traditional Chinese Medical Sciences

View full text Add to dashboard Cite

Chrysanthemum cinerariifolium (Trev.) Vis leaves extract reduces the level of Macrophages, Interleukin-17, and Keratinization in Oral squamous cell carcinoma rat model

Listiyana

Kristanti

Dharmayanti

et al. 2023

RJPT

View full text Add to dashboard Cite

Chrysanthemum cinerariifolium (Trev.) Vis. leaves have potential anticancer activity and are expected to inhibit oral squamous cell carcinoma (OSCC) progression. The present study aimed to determine the effect of ethanol extract of C. cinerariifolium leaves on the number of macrophages, IL-17 levels, and degree of keratinization in the OSCC rat model. Thirty male Sprague Dawley rats (weighing 80-160g, 2-3 months old) were randomly divided into five groups (n=6): (1) negative control (healthy rat, untreated); (2) positive control (OSCC rat); (3-5) dose 1, 2 and 3 (OSCC rat received 50, 100 and 200mg/kg BW of C. cinerariifolium leaves, respectively). The tongues of the rats were prepared for Hematoxylin-Eosin (HE) staining and immunohistochemistry. The number of macrophages was determined under a light microscope with 400x magnification. The level of IL-17 was examined using an ELISA assay. The degree of keratinization was calculated using the ImageJ software. All doses of C. cinerariifolium leaves extract could reduce the number of macrophages in OSCC rats. The levels of IL-17 in OSCC rats were decreased after being treated with 100 and 200mg/kg BW of C. cinerariifolium leaves extract. The high dose of C. cinerariifolium leaves extract (200mg/kg BW) reduced the degree of keratinization in the OSCC rat model. The study revealed that C. cinerariifolium leaves extract could inhibit the progression of OSCC by reducing macrophage, IL-17 and keratinization.

show abstract

Cancer Chemopreventive effect of Rodent Tuber (Typhonium flagelliforme (Lood) Bl) against DMBA-Induced Rats Breast Tumor

Cited by 3 publications

References 0 publications

Duku (Lansium domesticum) Leaves Extract Induces Cell Cycle Arrest and Apoptosis of HepG2 Cells via PI3K/Akt Pathways

Duku (Lansium domesticum) Leaves Extract Induces Cell Cycle Arrest and Apoptosis of HepG2 Cells via PI3K/Akt Pathways

Preclinical anticancer activity of Typhonium flagelliforme (Lodd.) Blume and its potential mechanism: A systematic review

Chrysanthemum cinerariifolium (Trev.) Vis leaves extract reduces the level of Macrophages, Interleukin-17, and Keratinization in Oral squamous cell carcinoma rat model

Contact Info

Product

Resources

About