Canine Parvovirus: Strain Difference in Haemagglutination Activity and Antigenicity

Abstract

SUMMARYCanine parvovirus (CPV) strains were compared for haemagglutination (HA) activity and antigenicity and were divided into two types by HA activity. Strains Cp49 and 29-F showed temperature-dependent HA, like feline panleukopenia virus (FPLV) and mink enteritis virus (MEV), whereas strains Sp-80 and Y-1 showed temperatureindependent HA with erythrocytes from eight species of animals. The results of a cross HA inhibition test using immunized rat sera suggested that of the two types of CPV those showing temperature-dependent HA were antigenically like FPLV and MEV whereas those showing temperature-independent HA were not. This antigenic difference between the two types was confirmed by a HA inhibition test with monoclonal antibodies. A chronological survey revealed that CPV isolates from earlier years have HA activity and antigenicity similar to those of FPLV and MEV, whereas current CPV isolates do not. There are some exceptional isolates from the transitional period which have similar antigenicity to FPLV and MEV but different HA activity. These results suggest that the haemagglutinin of CPV altered from one form resembling that of FPLV to a somewhat different structure during passage in dogs in nature.

Emergence, Natural History, and Variation of Canine, Mink, and Feline Parvoviruses

“…All virus types hemagglutinate erythrocytes from a variety of species at 4°C in buffers with pH values below 7.0 (Johnson, 1971;Goto et al, 1974;Goto, 1975;Mochizuki et al, 1978;Senda et al, 1988). FPV and MEV isolates hemagglutinated pig or rhesus monkey erythrocytes only at pH values below 7.0, and the pH optimum for most strains is below pH 6.6 (Carmichael et al, 1980;Moraillon and Moraillon, 1982).…”

Characterization of newly isolated rat viruses from asymptomatic laboratory rats

“…On the other hand, Mitra et al [6] reported that two antigenically indistinguishable strains of RV have distinct in vitro host cell specificity and in vivo pathogenic properties. In canine parvovirus (CPV), Characterization of newly isolated rat viruses UT-1 and UT-2, and RV-13 were examined for cross reaction in HI, ELISA and neutralization test a HI titer was expressed as the reciprocal of the highest serum dilution which hemagglutination was completely inhibited b ELISA titer was expressed as the reciprocal of the highest serum dilution resulting in, at least, a 0.2 difference in OD values between the test serum and control serum at 492 nm c Neutralization titer was expressed as the reciprocal of the highest serum dilution that showed complete inhibition of hemadsorption in at least 2 of 4 wells 119 Senda et al [9] described that temperature-dependent HA of CPV has varied in nature during the past several years. Our data suggested that RV also might yield variant types of HA accompanied with little change of antigenicity.…”

Oncolytic Properties of Some Orthopoxviruses, Adenoviruses and Parvoviruses in Human Glioma Cells

“…Пар-вовирус собак (CPV), штамм «Лайка-1993», выделенный в ГНЦ ВБ «Вектор», культивировали на клетках MDCK в соответствии с рекомендациями [12]. Титр вируса опре-деляли в реакции гемагглютинации с использованием эритроцитов макак-резус, как описано ранее [13].…”