Capillary zone electrophoretic separation and determination of imidazolic antifungal drugs

Arranz, A.; Echevarria, Camilo; Moreda, J.M.; Cid, Adolfo; Arranz, J. F.

doi:10.1016/s0021-9673(99)01228-5

Cited by 35 publications

(16 citation statements)

References 10 publications

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“…are suggested by the Food and Drug Administration (FDA) as therapeutic options for fungal infections. Among those, azole antifungal agents are the most rapidly expanding group [2,3].…”

Section: Introductionmentioning

confidence: 99%

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

2011

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Hollow fiber liquid-phase microextraction (HF-LPME) based on two immiscible organic solvents (n-dodecane as membrane solvent and 22 lL acetonitrile as acceptor phase) was evaluated for extraction and preconcentration of trace amounts of miconazole and clotrimazole as model azole antifungal drugs, prior to determination by gas chromatography (GC). The drugs were extracted from 24 mL sample solution into a thin layer of organic phase (n-dodecane) immobilized in hollow fiber pores, and then back-extracted into approximately 22 lL organic phase (acetonitrile) located in the lumen of the hollow fiber. After extraction, 1 lL acceptor phase was injected into a GC instrument. Under optimum conditions, limits of detection (LODs) ranged from 0.3-6.0 ng mL -1 , linear dynamic range of 1-400 ng mL -1 and relative standard deviations (RSD%) \5% were obtained. The developed method provided preconcentration factors of 639 and 496 for clotrimazole and miconazole, respectively. The applicability of the extraction method to different types of complicated matrices such as milk, plasma and urine samples was investigated. The results obtained results reveal that the proposed method is a good technique for extraction and determination of the target drugs in different matrices.

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“…are suggested by the Food and Drug Administration (FDA) as therapeutic options for fungal infections. Among those, azole antifungal agents are the most rapidly expanding group [2,3].…”

Section: Introductionmentioning

confidence: 99%

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

2011

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“…Several analytical methods have been developed for quantitative determination of ketoconazole, among them visible spectrophotometry (Abdel-Gawad, 1997), ultraviolet (UV) spectrophotometry (El-Shabouri et al, 1998;Kedor-Hackmann, Nery, Santoro, 1994), spectrofluorimetry (El-Shanawany et al, 1997), thin-layer chromatography (Roychowdhury, Das, 1996), supercritical fluid chromatography with UV detection (AshrafKhorassani, Levy, 1995), capillary electrophoresis with diode array detection (Arranz et al, 2000), high performance liquid chromatography (HPLC) using different detection modes such as UV (Al-Meshal, 1989;Abdel-Moety et al, 2002;Heyden et al, 2002), diode array (Koves, 1995) and electrochemical detection (Hoffman et al, 1988), and stripping voltametric and polarographic techniques (Arranz et al, 2003). However, no reference was found using first-derivative UV spectrophotometric determination of ketoconazole in emulsions.…”

Section: Introductionmentioning

confidence: 99%

First-derivative ultraviolet spectrophotometric and high performance liquid chromatographic determination of ketoconazole in pharmaceutical emulsions

Kedor-Hackmann¹,

Santoro²,

Singh³

et al. 2006

Rev. Bras. Cienc. Farm.

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“…Nowadays, the treatment of fungal diseases involves the use of several classes of antifungal agents, among which the azoles (imidazole-or triazole-based drugs) constitute an important group [2][3][4]. The compounds studied in this work, ketoconazole and terconazole, belong to this type of antifungal drugs.…”

Section: Introductionmentioning

confidence: 99%

Enantiomeric separation of ketoconazole and terconazole antifungals by electrokinetic chromatography: Rapid quantitative analysis of ketoconazole in pharmaceutical formulations

2005

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EKC using a neutral CD as chiral selector was applied in this work to the development of a method enabling the enantiomeric separation of ketoconazole and terconazole antifungals. The influence of different experimental conditions such as temperature, CD concentration, pH, and nature and concentration of the buffer on the enantiomeric resolution of the compounds studied was investigated. The use of 10 mM heptakis-(2,3,6-tri-O-methyl)-beta-CD in a 100 mM phosphate buffer (pH 3.5) with a temperature of 15 degrees C allowed the separation of the enantiomers of ketoconazole and terconazole with high resolution (R(s) > 2.0). The rapid separation of ketoconazole enantiomers with an analysis time less than 3 min was carried out after fitting some experimental parameters. The developed method was applied to the determination of ketoconazole in different pharmaceutical formulations.

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Capillary zone electrophoretic separation and determination of imidazolic antifungal drugs

Cited by 35 publications

References 10 publications

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

First-derivative ultraviolet spectrophotometric and high performance liquid chromatographic determination of ketoconazole in pharmaceutical emulsions

Enantiomeric separation of ketoconazole and terconazole antifungals by electrokinetic chromatography: Rapid quantitative analysis of ketoconazole in pharmaceutical formulations

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