Capture and selective release of multiple types of circulating tumor cells using smart DNAzyme probes

Zhang, Qianying; Wang, Wenjing; Huang, Shan; Yu, Sha; Tan, Tingting; Zhang, Jianrong; Zhu, Jun‐Jie

doi:10.1039/c9sc04309h

Cited by 35 publications

(32 citation statements)

References 78 publications

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“…(a) DNAzyme-based platform for the capture and release of circulating tumor cells. Reprinted with permission under a Creative Commons Attribution 3.0 unported license from ref . Copyright 2020 Royal Society of Chemistry.…”

Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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Since their discovery almost three decades ago, DNAzymes have been used extensively in biosensing. Depending on the type of DNAzyme being used, these functional oligonucleotides can act as molecular recognition elements within biosensors, offering high specificity to their target analyte, or as reporters capable of transducing a detectable signal. Several parameters need to be considered when designing a DNAzyme-based biosensor. In particular, given that many of these biosensors immobilize DNAzymes onto a sensing surface, selecting an appropriate immobilization strategy is vital. Suboptimal immobilization can result in both DNAzyme detachment and poor accessibility toward the target, leading to low sensing accuracy and sensitivity. Various approaches have been employed for DNAzyme immobilization within biosensors, ranging from amine and thiol-based covalent attachment to non-covalent strategies involving biotin–streptavidin interactions, DNA hybridization, electrostatic interactions, and physical entrapment. While the properties of each strategy inform its applicability within a proposed sensor, the selection of an appropriate strategy is largely dependent on the desired application. This is especially true given the diverse use of DNAzyme-based biosensors for the detection of pathogens, metal ions, and clinical biomarkers. In an effort to make the development of such sensors easier to navigate, this paper provides a comprehensive review of existing immobilization strategies, with a focus on their respective advantages, drawbacks, and optimal conditions for use. Next, common applications of existing DNAzyme-based biosensors are discussed. Last, emerging and future trends in the development of DNAzyme-based biosensors are discussed, and gaps in existing research worthy of exploration are identified.

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Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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“…2C) at temperatures below its melting point (T m = 33.5 1C). 50 However, when the temperature increases above T m , the aptamer will be temporarily denatured and lose its ability to bind to cells. Based on the matching responsiveness and similar critical temperatures of the hydrogel and aptamer, these two selected components can respond to temperature changes synergistically (Fig.…”

Section: Working Principle and Design Of The Icatch Devicementioning

confidence: 99%

A ‘smart’ aptamer-functionalized continuous label-free cell catch–transport–release system

Zhang

Wang

et al. 2021

J. Mater. Chem. B

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“…For instance, smart cyclic signal ampliative DNAzyme probes as ion sensing elements were designed to capture and release of CTCs. [ 151 ] In this context, the Sgc8c (Cu 2+ ‐DNAzyme‐sgc8c) and TD05 (Mg 2+ ‐DNAzyme‐TD05) aptamer modifications were utilized for capturing CCRF‐CEM and Ramos cells. The addition of Cu 2+ and Mg 2+ (cofactors in the reaction) catalyzed the cleavages of the substrate strands, thereby enabling the release of the captured cells.…”

Section: Smart Materials For the Release Of Ctcs/ctmmentioning

confidence: 99%

Smart Material‐Integrated Systems for Isolation and Profiling of Rare Cancer Cells and Emboli

Sagdic

İnci

2021

Adv Eng Mater

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This review presents a broad aspect of smart material‐integrated systems for isolating and profiling rare circulating tumor cells (CTCs) and circulating tumor microemboli (CTM) to provide physiological, biological, and mechanistic insights into cancer research. In particular, CTCs/CTM have emanated as essential pieces of evidence that can reveal clonal evolution, tumor heterogeneity, and disease progression within the metastatic cascade. Morphologies, cellular compositions, and rarity of CTCs/CTM make them difficult to track and isolate for profiling distinct molecular characteristics in the case of metastatic potential. Accordingly, with the advanced‐characterization techniques, examining the aspects of the specific surface markers of CTCs/CTM, epithelial‐to‐mesenchymal (EMT), mesenchymal‐to‐epithelial (MET) transitions, and timing of tumor cell dissemination would assist us to understand cancer biology and metastatic characteristics. Existing clinical and research methods for the enrichment and isolation of these sporadic cells depend on mainly conventional methods with low‐yield and expensive features. Owing to their specialized functions and analytical performances, smart material‐based technologies hold an enormous impact not only on cell detection, but also on cell isolation for downstream analyses. Herein, the main reasons for cell isolation are discussed and the recent developments in CTCs/CTM approaches for identifying further methods and future perspectives are elaborated on.

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Capture and selective release of multiple types of circulating tumor cells using smart DNAzyme probes

Abstract: Smart DNAzyme probes realized the capture and selective release of multiple circulating tumor cells.

Cited by 35 publications

References 78 publications

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

A ‘smart’ aptamer-functionalized continuous label-free cell catch–transport–release system

Smart Material‐Integrated Systems for Isolation and Profiling of Rare Cancer Cells and Emboli

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