Cascade Reaction-Mediated Assembly of Magnetic/Silver Nanoparticles for Amplified Magnetic Biosensing

Chen, Yiping; Xianyu, Yunlei; Dong, Mingling; Zhang, Jiangjiang; Zheng, Wenshu; Qian, Zhiyong; Jiang, Xingyu

doi:10.1021/acs.analchem.8b01138

Cited by 52 publications

(24 citation statements)

References 45 publications

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“…The limit of detection (LOD) of H 2 O 2 was calculated based on previous literature [27] via the following equation: LOD = 3S/M, where S is the standard deviation of blank samples and M is the slope of the linear curve between the absorbance at 652 nm and H 2 O 2 concentration. Eleven blank experiments were carried out as follows: 600 μL Fe 3 O 4 @LNPs (264 μg/mL), 300 μL of TMB (4 mM) and the corresponding volume (300 μL) of H 2 O replacing H 2 O 2 were mixed with 1.8 mL CPBS (0.1 M, pH = 3.0).…”

Section: Methodsmentioning

confidence: 99%

Fe3O4 Nanoparticles Loaded on Lignin Nanoparticles Applied as a Peroxidase Mimic for the Sensitively Colorimetric Detection of H2O2

Zhang

Guo

et al. 2019

Nanomaterials

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Lignin is the second largest naturally renewable resource and is primarily a by-product of the pulp and paper industry; however, its inefficient use presents a challenge. In this work, Fe3O4 nanoparticles loaded on lignin nanoparticles (Fe3O4@LNPs) were prepared by the self-assembly method and it possessed an enhanced peroxidase-like activity. Fe3O4@LNPs catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to generate a blue color, was observable by the naked eye. Under the optimal conditions, Fe3O4@LNPs showed the ability of sensitive colorimetric detection of H2O2within a range of 5–100 μM and the limit of detection was 2 μM. The high catalytic activity of Fe3O4@LNPs allows its prospective use in a wide variety of applications, including clinical diagnosis, food safety, and environmental monitoring.

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Section: Methodsmentioning

confidence: 99%

Fe3O4 Nanoparticles Loaded on Lignin Nanoparticles Applied as a Peroxidase Mimic for the Sensitively Colorimetric Detection of H2O2

Zhang

Guo

et al. 2019

Nanomaterials

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“…Author details 1 Department of Chemistry, University of Kurdistan, 66177-15175 Sanandaj, Iran. 2 Research Center for Nanotechnology, University of Kurdistan, 66177-15175 Sanandaj, Iran.…”

Section: Fundingmentioning

confidence: 99%

“…Enzymes, as biological macromolecules, are mainly composed of proteins, which can efficiently and selectively catalyze a diverse biochemical reactions [1,2]. They play a notable function in various fields, such as energy production processes, biosensing, the food industry, and biofuels [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Multienzymes activity of metals and metal oxide nanomaterials: applications from biotechnology to medicine and environmental engineering

Alizadeh

Salimi

2021

J Nanobiotechnol

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With the rapid advancement and progress of nanotechnology, nanomaterials with enzyme-like catalytic activity have fascinated the remarkable attention of researchers, due to their low cost, high operational stability, adjustable catalytic activity, and ease of recycling and reuse. Nanozymes can catalyze the same reactions as performed by enzymes in nature. In contrast the intrinsic shortcomings of natural enzymes such as high manufacturing cost, low operational stability, production complexity, harsh catalytic conditions and difficulties of recycling, did not limit their wide applications. The broad interest in enzymatic nanomaterial relies on their outstanding properties such as stability, high activity, and rigidity to harsh environments, long-term storage and easy preparation, which make them a convenient substitute instead of the native enzyme. These abilities make the nanozymes suitable for multiple applications in sensing and imaging, tissue engineering, environmental protection, satisfactory tumor diagnostic and therapeutic, because of distinguished properties compared with other artificial enzymes such as high biocompatibility, low toxicity, size dependent catalytic activities, large surface area for further bioconjugation or modification and also smart response to external stimuli. This review summarizes and highlights latest progress in applications of metal and metal oxide nanomaterials with enzyme/multienzyme mimicking activities. We cover the applications of sensing, cancer therapy, water treatment and anti-bacterial efficacy. We also put forward the current challenges and prospects in this research area, hoping to extension of this emerging field. In addition to therapeutic potential of nanozymes for disease prevention, their practical effects in diagnostics, to monitor the presence of SARS-CoV-2 and related biomarkers for future pandemics will be predicted.

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“…According to a report, one kind of magnetic/ silver nanoassemblies (Ag-MNPs) mediated by an enzyme cascade reaction can greatly enhance the state change of MNPs (from dispersed state to aggregated state). Therefore, it can improve the sensitivity of the conventional MRS sensor dramatically (Chen et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle Assembly

et al. 2021

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Conventional immunoassay methods have their common defects, such as tedious processing steps and inadequate sensitivity, in detecting whole blood. To overcome the above problems, we report a microfluidic chip–based magnetic relaxation switching (MRS) immunosensor via enzyme-mediated nanoparticles to simplify operation and amplify the signal in detecting whole blood samples. In the silver mirror reaction with catalase (CAT) as the catalyst, H2O2 can effectively control the production of Ag NPs. The amount of Ag NPs formed further affects the degree of aggregation of magnetic nanoparticles (MNPS), which gives rise to the changes of transverse relaxation time (T2). Both sample addition and reagent reaction are carried out in the microfluidic chip, thereby saving time and reagent consumption. We also successfully apply the sensor to detect alpha-fetoprotein (AFP) in real samples with a satisfied limit of detection (LOD = 0.56 ng/ml), which is superior to the conventional ELISA.

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Cascade Reaction-Mediated Assembly of Magnetic/Silver Nanoparticles for Amplified Magnetic Biosensing

Cited by 52 publications

References 45 publications

Fe3O4 Nanoparticles Loaded on Lignin Nanoparticles Applied as a Peroxidase Mimic for the Sensitively Colorimetric Detection of H2O2

Fe3O4 Nanoparticles Loaded on Lignin Nanoparticles Applied as a Peroxidase Mimic for the Sensitively Colorimetric Detection of H2O2

Multienzymes activity of metals and metal oxide nanomaterials: applications from biotechnology to medicine and environmental engineering

A Microfluidic Chip-Based MRS Immunosensor for Biomarker Detection via Enzyme-Mediated Nanoparticle Assembly

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