Cathodic stripping voltammetry of pipemidic acid and ofloxacin in pharmaceutical dosages and human urine

Abstract

Sensitive cathodic stripping voltammetric methods have been developed for two quinolone antibacterial drugs, pipemidic acid (PIP) and ofloxacin (OFL) using hanging mercury drop electrode as working electrode vs. Ag/AgCl reference electrode. The methods were developed for the determination of drugs individually as well as simultaneously. 0.1 M and 0.01 M hydrochloric acid was used as medium for PIP and OFL, respectively, 0.1 M potassium chloride was used as base electrolyte. Reduction waves were observed for PIP within -700 mV to -800 mV and for OFL within -1100 mV to -1200 mV. Linear calibration ranges for PIP and OFL were observed within 10-100 µg ml -1 with detection limits of 50 ng ml -1 and 1 µg ml -1 , respectively. Relative standard deviations (RSD) for the analysis of 10 µg ml -1 of PIP and OFL (n = 6) were 0.5% and 1.4%, respectively. The presence of glucose, lactose, sorbitol, gum arabic, starch, magnesium stearate, methylparaben and propylparaben did not affect the determinations of both PIP and OFL. The methods were used for the analysis of pharmaceutical preparations and the results indicated relative deviation of 0.5-5.5% from labeled values with RSD within 0.49-2.5%. PIP and OFL could also be determined simultaneously, and were determined from spiked human urine.

Recent advances in the analysis of antibiotics by CE and CEC

“…On the other hand, different detection systems such as UV, ECL, CL, C 4 D, PGD, LIF, and MS have been employed for the determination of quinolones and fluoroquinolones. Regarding UV detection, a simple and reliable methodology using CZE‐UV was developed for the determination of pipemidic acid and ofloxacin 34. The use of tetraborate buffer at pH 10.0 allowed the separation of ofloxacin and pipemidic acid in the presence of other drugs (pseudo‐ephedrine and piroxicam) and vitamins (VB 1 , VB 6 , and VB 12 ) in <9 min.…”

Square-Wave Voltammetry of Human Blood Serum

“…The experimental data presented above demonstrate the effectiveness of SWV in providing insight into the electrochemistry of a variety of species in a complex medium when applied at a macroscopic electrode with superior electrocatalytic properties such as EPGE, which is a commercially available and conventional electrode. Despite numerous analytical studies aimed at detecting speci c small biomolecules or drugs in a serum sample [38][39][40], this study is the rst to use SWV in a spectroscopy-like manner to assign the voltammetric pro le of a serum sample to the dominant underlying electrode processes. It is worth mentioning that attempts to study blood serum voltammetrically date back to the early works of Koryta et al [5,6], where CV was applied at a Pt electrode.…”

Application of Electrochemical Methods for Analysis of Fluoroquinolones Antibacterial Agents and Fluoroquinolones-DNA Interactions

“…Sensitive cathodic stripping voltammetric methods have been developed for two quinolone antibacterial drugs, pipemidic acid and ofloxacin using hanging mercury drop electrode [42]. The methods were developed for the determination of drugs individually as well as simultaneously.…”