Cell cycle analysis of rat lymphocytes cultured with phytohemagglutinin in diffusion chambers

Ribonucleic Acid and Phytohemagglutinin on Rat Leukocyte Cultures within Diffusion Chambers

“…However, these cells have been reported to grow once stimulated with PHA, both in vitro [16], or in vivo [8]. In the present ex periments, it has been possible to obtain high values of blast transforma tion, 55 and 59°/o, and mitotic index, 1.5 and 2, after 72 and 96 h of PHA stimulation, respectively.…”

Diffusion chamber hematopoiesis in marrow and spleen cells from mice infected with rauscher leukemia virus (RLV‐A)

“…The diffusion chamber culture method employed in these studies was first introduced by Algire et al (1954) and later modified by Berman and Kaplan (1959). The technique has been further refined by Johnson et al (1967Johnson et al ( ,1969Johnson et al ( , 1972, Benestad (1970), and Boyum and Borgstrom (1970).…”

Growth Characteristics of the Shay Chloroleukemia in Diffusion Chambers

“…More recently, diffusion chambers have been employed to investigate the response of rodent and human leukocytes to phytoheniagglutinin (PHA) and to study the kinetics of PHA-stimulated lymphocyte proliferation using tritiated thymidine autoradiography (7)(8)(9). The latter studies involved the serial sacrifice of rats bearing intraperitoneal diffusion chambers harvested at specific time intervals after isotope administration.…”

“…Wistar rats and Swiss mice were anesthetized by Metafane inhalation ( Pittman-Moore, Indianapolis) and the chambers were introduced into the peritoneal cavity by laparotomy. Two milliliters of medium 199 were injected into the peritoneal cavity after closure of the wound to retard development of connective tissue lesions around the chambers (7,8).…”