Cellular Protective and Antioxidative Acivities of Parthenocissus tricuspidata Stem Extracts

Abstract

In this study, the cellular protective effects on HaCaT cells and human erythrocytes and antioxidative effects of P. tricuspidata stem extracts were investigated. The ethyl acetate (50 µ g/mL) and aglycone fraction (25 µ g/mL) of P. tricuspidata stem extracts doesn't show any characteristics of cytotoxicity. When HaCaT cells were treated with 10 mM H2O2 and 30 µ M rose bengal, the ethyl acetate (6.25 ∼ 50 µ g/mL) and aglycone (6.25 ∼ 25 µ g/mL) fraction protected the cells against the oxidative damage in a concentration dependent manner. The P. tricuspidata stem extracts showed more prominent cellular protective effect than (+)-α-tocopherol, known as lipid antioxidant at 10 µ g/mL. The ethylacetate fraction of P. tricuspidata stem extracts (18.5 µ g/mL) showed more free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC550). Reactive oxygen species (ROS) scavenging activity (OSC50) of P. tricuspidata stem extracts on ROS generated in Fe 3+ -EDTA/H2O2 system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate (1.72 µ g/mL) and the aglycone fraction (1.53 µ g/mL) showed similar ROS scavenging activity of L-ascorbic acid (1.50 µ g/mL). These results indicate that extract/fractions of P. tricuspidata stem extracts can function as natural cytoprotective agents and antioxidants in biological systems, particularly skin exposed to UV radiation by protecting cellular membrane against ROS.

Cellular Antioxidant Activity and Whitening Effects of Dendropanax morbifera Leaf Extracts

“…멜라닌은 각질형성세포로 이동되어 자외선 등에 의한 피부의 노화나 일광각화증을 억 제하여 피부를 보호하는 긍정적인 기능을 가지고 있는 반면 에, 과도하게 생성될 경우 피부에 색소가 침착되어 기미, 주 근깨를 형성하며, 이와 같은 병변은 나아가 피부암의 원인이 되기도 한다 [6,9,20,21,23]. 따라서 피부노화를 지연 또는 억제시키기 위해서 자외선 을 차단하거나 피부에서의 ROS의 생성 및 생성된 ROS를 효 율적으로 제거하여 ROS로부터 세포 및 조직을 보호할 수 있 는 항산화제 개발과 함께 밝고 아름다운 피부를 유지하기 위 한 새로운 미백제 개발이 최근 절실히 요구되고 있는 실정 이다 [8,12,18].…”

Inhibitory Effects of Dendropanax Morbifera Leaf Extracts on Melanogenesis through Down-Regulation of Tyrosinase and TRP-2

“…However, excess generation of melanin causes dye deposition on the skin accompanied by formation of spots and freckles; such lesions often lead to skin cancer [7][8][9][10][11]. Therefore, new skin whitening agents are required to help maintain bright and healthy skin [12][13][14].…”

Inhibitory effects of mung bean (Vigna radiata L.) seed and sprout extracts on melanogenesis

“…However, if excessive melanin is produced, or if the function of skin cells is reduced via aging, melanin can be deposited on the surface of the skin, appearing as spots and freckles that are induced by toxicity caused by melanin precursors, which can also induce cell death (12)(13)(14). Therefore, development of a new whitening agent would be helpful for maintenance of bright and healthy skin (15)(16)(17).…”