Cellulases Production by a Trichoderma sp. Using Food Manufacturing Wastes

Abstract

The cost of cellulase enzymes is a main contributor to the operational cost of a biorefinery producing ethanol from lignocellulosic material. Therefore, onsite production of enzymes using low-value substrates might be an option to make a bio-based facility more economical, while improving environmental sustainability. Food manufacturing wastes (FMWs), such as olive mill solids, tomato pomace, and grape pomace, are some of the main wastes produced by the food industry in Chile. FMWs are mostly composed of lignocellulosic material, which is primarily made of cellulose. A fungal strain obtained from olive stones was identified as a Trichoderma sp. and characterized by molecular and morphological techniques. This strain was able to grow on three FMWs in both liquid and solid cultures. In liquid cultures, cellulase and β-glucosidase activities from the culture supernatants were quantified. Identification of extracellular proteins using mass spectrometry revealed the presence of endoglucanases, exoglucanases, and β-glucosidases. Cellulase production from agroindustrial residues could be an excellent opportunity to utilize FMWs as well as decrease enzyme production costs in biorefinery processes.

Molecular identification of cellulase-producing thermophilic fungi isolated from Sungai Pinang hot spring, Riau Province, Indonesia

“…The higher the bootstrap repetition rate, the higher the accuracy of the phylogenetic tree obtained. Several studies also used 10.000 bootstrap replications in fungal and bacterial testing (Krimitzas et al 2013;Anwar et al 2016;Gordillo-Fuenzalida et al 2019). The size of the LBKURCC293 isolate in the ITS region was 609 and based on the phylogenetic analysis, the isolate was grouped with A. fumigatus.…”

Extracting flavonoid from Ginkgo biloba using lignocellulolytic bacteria Paenarthrobacter sp. and optimized via response surface methodology

“…Several studies have shown their potential in extracting various types of value‐added and bioactive compounds such as phenolic compounds, 34 carbohydrates, 35 fruit juice, 36 and pigments 37 . So far, many fungi and bacteria have been found capable of producing lignocellulolytic enzymes such as cellulases, 38–41 hemicellulases, 42–44 peroxidases, and laccases 45–49 …”

“…Several studies have shown their potential in extracting various types of value-added and bioactive compounds such as phenolic compounds, 34 carbohydrates, 35 fruit juice, 36 and pigments. 37 So far, many fungi and bacteria have been found capable of producing lignocellulolytic enzymes such as cellulases, [38][39][40][41] hemicellulases, [42][43][44] peroxidases, and laccases. [45][46][47][48][49] The utilization of lignocellulolytic enzymes could contribute to improving the extraction of flavonoids from Ginkgo biloba, 26,27,50,51 and fermentation using lignocellulosedegrading microorganisms could be another innovative approach to improving the extraction of Ginkgo biloba flavonoids.…”

Estimation of glucosamine in biomass of Trichoderma reesei cultivated on lignocellulosic substrates

“…Trichoderma and Aspergillus are the most important producers of hydrolytic enzymes of lignocelluloses including cellulase, amylase and hemicellulase, and ligninolytic enzymes for industrial purposes [9–12]. Many researchers have studied the SSF of lignocellulosic materials using Trichoderma reesei as a highly efficient producer of lignocellulose‐degrading enzymes [13–15].…”