Celsior Preserved Cardiac Mechanoenergetics Better Than Popular Solutions in Canine Hearts

Oshima, Yu; Mohri, Satoshi; Shimizu, Juichiro; Iribe, Gentaro; Imaoka, Takeshi; Fujinaka, Waso; Kiyooka, Takahiko; Ishino, Kozo; Sano, Shunji; Kajiya, Fumihiko; Suga, Hiroyuki

doi:10.1016/j.athoracsur.2005.07.067

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…Although there are suitable preservation techniques and solutions for the liver and kidney, there remains a need for better methods to protect the heart for periods longer than 4 -5 h (9,10,32,39,50). More popular clinically used solutions include ViaSpan (22, 28, 51) (also called UW solution), HTK (histidine-tryptophan-ketoglutarate based, or Bretschneider solution) (16,41), Celsior (antioxidant-based solution with mannitol, reduced glutathione, plus high Mg 2ϩ , lactobionate, and glutamate) (5,17,20,33,34), and St. Thomas Hospital solution (STH; a high-K ϩ , high-Mg ϩ , low-Ca 2ϩ , lidocainecontaining solution) (4,8,21,25); others are Euro-Collins and Stanford solutions.…”

Section: Discussionmentioning

confidence: 99%

Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26°C: comparison to ViaSpan solution

Stowe

Camara²,

Heisner³

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

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There is no suitable solution to preserve hearts for longer than 5 h between donor explant and recipient implant. Lifor is a fully artificial preservation medium containing both a nonprotein oxygen and nutrient carrier (nanoparticles) and cellular nutrients, including amino acids and sugars. We proposed that recirculated Lifor solution would satisfactorily preserve guinea pig isolated hearts perfused at low flow with no added O(2) at room temperature for 10 h. Hearts were isolated from 21 guinea pigs and perfused with Krebs-Ringer (KR) solution (97% O(2) and 3% CO(2)) at 37 degrees C. Heart rate, inflow and outflow O(2) tension, coronary flow, left ventricular pressure (LVP), and maximal and minimal rate of change in LVP (dLVP/dt) were measured. After baseline measurements, hearts were perfused with recirculated Lifor or ViaSpan equilibrated with room air at 15% of control flow at 26 degrees C for 10 h. Hearts were then perfused at 100% flow with KR for 2 h at 37 degrees C. A time control (untreated) group was perfused only with KR solution for 15 h. Lifor arrested and protected hearts against diastolic contracture and maintained a low O(2) extraction. Compared with time controls, Lifor led to a higher developed LVP and coronary flow; %O(2) extraction and cardiac efficiency were similar between these two groups. Hearts similarly treated with ViaSpan exhibited diastolic contracture and lower %O(2) extraction during treatment and, upon reperfusion with KR, exhibited continued diastolic contracture, no return of heart rate or contractility, low coronary flow, low %O(2) extraction, and marked infarction. For long-term cardiac protection, a suitable preservation solution recirculated at low flow and room temperature without supplemental O(2) would reduce the support apparatus required for transport. Lifor was far superior to ViaSpan in meeting these requirements.

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Section: Discussionmentioning

confidence: 99%

Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26°C: comparison to ViaSpan solution

Stowe

Camara²,

Heisner³

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

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“…Although there are suitable long-term preservation techniques for the liver and kidney, hearts cannot be well protected for periods of more than 4 or 5 hours 16–20. More popular experimentally tested and clinically used solutions include ViaSpan21–24 (also called UW solution), HTK (histidine–tryptophan–ketoglutarate-based solution, or Bretschneider’s),25,26 Celsior (anti-oxidant–based solution with mannitol, reduced glutathione, plus high Mg 2+ , lactobionate and glutamate)27–31 and STH (St Thomas’ Hospital, a high K + , high Mg + , low Ca 2+ , lidocaine-containing solution)32–35; others include Euro-Collins and Stanford solutions. Most of these solutions have a “cardioplegic” (high [K + ]) base to arrest the heart.…”

Section: Discussionmentioning

confidence: 99%

Low-flow Perfusion of Guinea Pig Isolated Hearts With 26°C Air-saturated Lifor Solution for 20 Hours Preserves Function and Metabolism

Stowe

Camara

Heisner

et al. 2008

The Journal of Heart and Lung Transplantation

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Background-Donor human hearts cannot be preserved for >5 hours between explantation and recipient implantation. A better approach is needed to preserve transplantable hearts for longer periods, ideally at ambient conditions for transport. We tested whether Lifor solution could satisfactorily preserve guinea pig isolated hearts perfused at low flow with no added oxygen at room temperature for 20 hours.

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“…For example, as an intracellular-type solution, Celsior solution has certain advantages for heart preservation. 3,4 In addition, the inclusion of an ATP-sensitive potassium channel opener, including adenosine, 5 pinacidil, 6 and diazoxide, into the preservation solution confers maximal protection to endothelial function of the coronary arteries 7 and overall heart preservation and maintenance of cardiac function. 8 Regarding the preservation technique, a simple low-temperature immersion method is still most commonly used in the clinical practice.…”

mentioning

confidence: 99%

Comparative Analysis of Preservation Method and Intermittent Perfusion Volume on the Expression of Endothelial and Inflammatory Markers by Coronal Artery and Myocardium in Porcine Donor Hearts

Zhou

Wang

et al. 2014

ASAIO Journal

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Although continuous perfusion of donor hearts for preservation during transportation has been widely applied, intermittent perfusion has been suggested as an alternative. The aim of this study was to identify the optimal intermittent perfusion protocol by investigating the effects of perfusion volume on endothelial and inflammatory marker expression by the coronary artery. Donor porcine hearts were perfused with various volumes of Celsior solution supplemented with diazoxide (50, 100, 150, 200, and 250 ml) every 2 h for 30 min each for a 10 h period. The effects on cardiomyocytes and vascular endothelial cell morphology and marker expression were compared to the immersion control group. Whereas an incomplete endothelial cell layer with disorganized connective tissue was observed in the control and 50, 100, and 150 ml intermittent perfusion groups, transmission electron microscopic analysis revealed a complete endothelial cell layer in the intima with an organized subendothelium. A perfusion volume-dependent increase in eNOS expression that coincided with a decrease in ET-1, ICAM-1, vWF, and P-selectin expression was detected (all p < 0.01). Intermittent perfusion with 200 ml of Celsior solution every 2 h conferred protective effects simultaneously to the coronary arteries and myocardium on the porcine donor heart over a clinically relevant preservation period.

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Celsior Preserved Cardiac Mechanoenergetics Better Than Popular Solutions in Canine Hearts

Abstract: Celsior showed better protective effects on cardiac mechanoenergetics than St. Thomas' Hospital and University of Wisconsin solutions in the acute phase of heart transplantation.

Cited by 4 publications

References 20 publications

Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26°C: comparison to ViaSpan solution

Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor solution at 26°C: comparison to ViaSpan solution

Low-flow Perfusion of Guinea Pig Isolated Hearts With 26°C Air-saturated Lifor Solution for 20 Hours Preserves Function and Metabolism

Comparative Analysis of Preservation Method and Intermittent Perfusion Volume on the Expression of Endothelial and Inflammatory Markers by Coronal Artery and Myocardium in Porcine Donor Hearts

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