Changes in phenol content during strawberry (Fragaria×ananassa, cv. Chandler) callus culture

Abstract

Total soluble phenols, soluble flavanols, (+)-catechin, ferulic acid and 1-O-feruloyl-beta-d-glucose were analyzed during the development of a strawberry (Fragariaxananassa, cv. Chandler) callus culture. The time-course changes of the different phenols assayed were well correlated with callus growth and morphology. The changes in polyphenol oxidase (EC 1.10.3.1-2) and beta-glucosidase (EC 3.2.1.21) activities in the callus were also examined. The total phenol, soluble flavanols and (+)-catechin contents were high during the preexponential and exponential phases of growth. The subsequent decrease in (+)-catechin concentration coincided with high levels of polyphenol oxidase activity. The 1-O-feruloyl-beta-d-glucose content was highest as callus growth ceased, and its subsequent decrease was accompanied by the increased production of ferulic acid. This increase in ferulic acid was accompanied by an increase in beta-glucosidase activity. The ferulic acid content decreased at the end of culture, when callus growth had stopped and showed clear symptoms of senescence. This decrease in the ferulic acid concentration was accompanied by an increase in the levels of ferulic acid bound to cell wall components.

Effects of Ascorbic Acid in Controlling Lethal Browning in <i>in Vitro</i> Culture of <i>Brahylaena huillensis</i> Using Nodal Segments

“…Thus, accumulation of phenolic compounds in medium adversely affects the growth and survival of in vitro explants. [34,35] showed that accumulation of these compounds leads to browning and possibly death of the explants. Moreover, oxidized phenolic compounds may inhibit enzyme activity and result in the darkening of the culture medium and subsequent lethal browning of explants [36,37].…”

The use of organic biostimulants in hot pepper plants to help low input sustainable agriculture

“…Total phenols in RG extracts were determined according to Arnaldos et al [22]. Specifically, RG (1 mL) was maintained in ice with pure methanol (1:1, v/v) for 30 min and centrifuged at 5,000g for 30 min at 4°C.…”

In vitro regeneration ability of diploid and autotetraploid plants of Cichorium intybus L.

“…Phenolic compounds, especially oxidized phenolics, may contribute to the inhibition of enzymatic activities, resulting in the darkening of the culture medium and subsequent lethal browning of explants [32,33]. It has been reported that oxidized phenolics generally affect in vitro proliferation and regeneration negatively [34].…”