Characteristics of Platelet Lysate Compared to Autologous and Allogeneic Serum Eye Drops

Abstract

Platelet lysate produced from platelet apheresis components has been proposed as an alternative to serum eye drops in the treatment of ocular surface disease. This study compared the effects of platelet lysate and serum on growth factor, cytokine and nanoparticle concentrations, and corneal epithelial cell proliferation. Methods: The concentration of growth factors, cytokines, and nanoparticles in platelet lysates manufactured from either fresh or expired platelet apheresis concentrations collected with Trima or Haemonetics technology was characterized and compared with those of allogeneic, autologous, and fetal calf serum. The ability to promote corneal epithelial cell proliferation and wound healing was tested in vitro. Results: Platelet lysate enriched the amount of transforming growth factor β1, plateletderived growth factor-AB and-BB, fibroblast growth factor, and epidermal growth factor compared with the two sera groups. The concentrations of insulin-like growth factor 1, hepatocyte growth factor, and fibronectin were significantly lower than in sera. There were no differences in nanoparticle concentrations. There was no significant difference in corneal epithelial cell proliferation. Platelet lysates were comparable to fetal calf serum in accelerating corneal epithelial wound healing in vitro. Conclusions: Fresh and expired platelet lysates from the Trima and Haemonetics systems had higher growth factor concentrations than sera. The ability of platelet lysates to promote corneal epithelial cell proliferation and wound healing was equivalent to sera. Translational Relevance: Platelet lysates may serve as an efficient and reliable source of human growth factors for the treatment of ocular surface diseases.

Development of a Platelet Lysate–Based Printable, Transparent Biomaterial With Regenerative Potential for Epithelial Corneal Injuries

“…To minimize irregularity caused by batch-batch variation in the scratch wound assay, we used 5% CCP4 and showed that hPL significantly increased the rate of wound closure compared to FBS. Our results are consistent with previous published results 27 , 28 showing hPL has a positive effect on epithelial cell proliferation and wound healing. These findings also suggest that standard preparation protocols and strict quality control is essential when using hPL in cell culture.…”

A review of current advancements for wound healing: Biomaterial applications and medical devices

“…It has been used to produce sponge-like dressings, 124 scaffolds, 125 bioactive gels, 126,127 and eye drops. 128,129 Recently, a core-shell structure particle-based dressing was designed with a calcium alginate shell and embedded in an alginate matrix. 130 PL and an antibiotic were loaded in the external matrix as active components for the treatment of chronic skin ulcers.…”

“…In comparison with platelet‐rich plasma or platelet‐rich fibrin, PL has the advantage of limiting the variability of individuals. It has been used to produce sponge‐like dressings, 124 scaffolds, 125 bioactive gels, 126,127 and eye drops 128,129 . Recently, a core‐shell structure particle‐based dressing was designed with a calcium alginate shell and embedded in an alginate matrix 130 .…”

Regenerative effect of expired platelet concentrates in human therapy: An update

“…An increasing number of centers worldwide are producing serum eye drops from allogeneic blood donors, as highlighted in several publications, including in this journal [30][31][32][33][34]. Demonstrations have been recently provided that HPL produced from apheresis PC has high levels of growth factors and promotes corneal epithelial cell proliferation and wound healing at least as efficiently as serum [35]. Clinical data show the benefit of HPL prepared from allogeneic cord blood [36].…”