1997
DOI: 10.1006/excr.1997.3573
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Characterization of Cholesterol-Free Insect Cells Infectible by Baculoviruses: Effects of Cholesterol on VSV Fusion and Infectivity and on Cytotoxicity Induced by Influenza M2 Protein

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Cited by 34 publications
(34 citation statements)
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“…Analysis by GC/MS confirmed an overall purity of 92 ϩ %, with minor contamination by lophenol, a diene, and a 4,4 dimethylsterol. The disappearance of the doublet at ␦ 5.2 ppm in the 1 H-NMR spectrum indicated the essentially complete conversion of lophenol to the ⌬ 8 (14) isomer. The vinylic carbons of ⌬ 8(14) sterol (Scheme 1, part 2) in the 13 C-NMR spectrum appeared at ␦ 126.0 and 142.2 ppm, whereas those for lophenol (Scheme 1, part 1) appeared at ␦ 117.4 and 139.2 ppm.…”
Section: Synthesis Of 4-␣ -Methyl-5-␣ -Cholest-8(14)-en-3 ␤ -Olmentioning
confidence: 99%
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“…Analysis by GC/MS confirmed an overall purity of 92 ϩ %, with minor contamination by lophenol, a diene, and a 4,4 dimethylsterol. The disappearance of the doublet at ␦ 5.2 ppm in the 1 H-NMR spectrum indicated the essentially complete conversion of lophenol to the ⌬ 8 (14) isomer. The vinylic carbons of ⌬ 8(14) sterol (Scheme 1, part 2) in the 13 C-NMR spectrum appeared at ␦ 126.0 and 142.2 ppm, whereas those for lophenol (Scheme 1, part 1) appeared at ␦ 117.4 and 139.2 ppm.…”
Section: Synthesis Of 4-␣ -Methyl-5-␣ -Cholest-8(14)-en-3 ␤ -Olmentioning
confidence: 99%
“…The key step in the synthesis is the PtO 2 -catalyzed isomerization of the 7(8)-double bond of lophenol ( Scheme 1 , part 1) to the more stable 8 (14) isomer (Scheme 1, part 2) (22).…”
Section: Synthesis Of 4-␣ -Methyl-5-␣ -Cholest-8(14)-en-3 ␤ -Olmentioning
confidence: 99%
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“…In addition to its role in entry of non enveloped the role of membrane rafts in entry of enveloped viruses has been investigated in studies using influenza virus (Orthomyxoviridae) [57][58][59][60][61], human immunodeficiency virus type 1 (HIV-1; Retroviridae) [62][63][64][65][66][67], human T lymphotropic virus 1 (HTLV-1; Retroviridae) [68,69], Ebola virus (Filoviridae) [70], Marburg virus (Filoviridae) [70,71], Epstein-Barr virus (EBV; herpesviridae) [72,73], herpes simplex virus-1 (HSV-1; herpesviridae) [74] including porcine herpesvirus-1 pseudorabies virus [75], human herpesvirus-6 (HHV-6; Herpesviridae) [76], human herpesvirus-8 (HHV-8; Herpesviridae) [77], vaccinia virus (Poxviridae) [78], coronavirus including severe acute respiratory syndrome coronavirus (SARS-CoV; Coronaviridae) [79][80][81][82][83][84][85], West Nile virus (WNV; Flaviviridae) [86], dengue virus (DEN; Flaviviridae) [87][88][89], Japanese encephalitis virus (JEV; Flaviviridae) [89], human hepatitis C virus (HCV; Flaviviridae) [90,91], Semliki Forest virus (Togaviridae) [92][93][94][95], Sindbis virus (Togaviridae) …”
Section: Role Of Membrane Rafts In Virus Entrymentioning
confidence: 99%
“…After transportation of the virus to late endosomes, low-pHdependent conformation change of HA induces membrane fusion of the viral envelope with the endosomal membrane. Then viral ribonucleoprotein complexes (RNP) including the viral genome are released to the cytoplasm of host cells by proton influx of viral ion channel M2 protein that requires binding with cholesterol [57,104]. Similar to the entry process of influenza virus, capsid-like core particles of hepatitis B virus (Hepadnaviridae) are internalized through clathrin-dependent and raft-independent endocytosis [105].…”
Section: Role Of Membrane Rafts In Virus Entrymentioning
confidence: 99%