Characterization of diversity in<i>Colletotrichum acutatum</i><i>sensu lato</i>by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility

Guerber, John C.; Liu, Bo; Correll, James C.; Johnston, Peter R.

doi:10.1080/15572536.2004.11833047

Cited by 244 publications

(51 citation statements)

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“…The translation elongation factor 1-α gene (TEF1) and actin gene (ACT) of P. capitalensis and P. citricarpa were amplified using primers EF1-728F and EF1-986R, and ACT-512F and ACT-783R, respectively (Carbone & Kohn, 1999). The glyceraldehyde-3-phosphate dehydrogenase (GPDH) gene was amplified using primers GDF1 (Guerber et al, 2003) and Gpd2-LM (Myllys et al, 2002) or GDR1 (Guerber et al, 2003) for P. capitalensis isolates, and GDF1 and GPDHR2 (Glienke, et al, 2011) for the P. citricarpa isolates (Table 2).…”

Section: Dna Amplificationmentioning

confidence: 99%

Genetic variation among Phyllosticta strains isolated from citrus in Florida that are pathogenic or nonpathogenic to citrus

Maridueña-Zavala¹,

Er²,

Goss³

et al. 2014

Trop. plant pathol.

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Citrus black spot is an emerging disease in Florida since 2010. The causal agent is Phyllosticta citricarpa (teleomorph Guignardia citricarpa), but non-pathogenic P. capitalensis (teleomorph often referred to as G. mangiferae) is often isolated from black spot lesions. Florida isolates of P. citricarpa and P. capitalensis from citrus have not been characterized in detail. In this study, Phyllosticta species isolated from Florida citrus were compared with worldwide isolates using multi-locus sequencing of four conserved loci (rDNA ITS, TEF1, ACT, and GPDH genes). Moreover, the diversity within the two Phyllosticta species was compared based on the same four loci. DNA sequences of P. citricarpa and P. capitalensis were clearly distinct, coinciding with other P. citricarpa and P. capitalensis sequences from different continents. The species showed different population structures in Florida. P. citricarpa isolates did not exhibit genetic variation and were similar to strains from other continents. In contrast, Florida P. capitalensis isolates were distributed over five sequence groups. This study did not point to the potential origin of P. citricarpa and P. capitalensis in Florida. More variable genetic markers and isolates from various continents would be required to track the possible movement of these Phyllosticta species.

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Section: Dna Amplificationmentioning

confidence: 99%

Genetic variation among Phyllosticta strains isolated from citrus in Florida that are pathogenic or nonpathogenic to citrus

Maridueña-Zavala¹,

Er²,

Goss³

et al. 2014

Trop. plant pathol.

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“…Indeed, isolate C. acutatum HB05 from pepper was recently found to be different from strawberry and citrus isolates collected in Florida in Dr. James Correll's laboratory at the University of Arkansas (T. L. Harp & J. C. Correll, unpublished). Furthermore, C. acutatum HB05 was found to be most closely related to isolates recovered from pepper in Taiwan by RFLP analysis (J. C. Correll, personal communication), which were grouped into the mtDNA haplotype D3 (Guerber et al 2003). Our field data demonstrated that pepper was the only crop infected by C. acutatum, and population analysis suggests clonality; one could hypothesize that isolates of C. acutatum recovered from pepper are host-specific.…”

Section: Discussionmentioning

confidence: 82%

“…More recently, enhanced molecular techniques beyond the traditional use of ITS sequence data have been employed in studies of Colletotrichum and have further delineated species boundaries in this taxonomically complex genus (Correll et al 2007;Crouch et al 2009;Guerber et al 2003;Liu & Correll 2000). Although a great deal of work has been done to identify and distinguish species of Colletotrichum based on the conserved ITS region (Adaskaveg & Hartin 1997;Freeman et al 2000;Sreenivasaprasad et al 1996), additional approaches have been employed more recently to identify and examine sub-species populations within C. acutatum and these subgroups have been shown in some studies to correlate with differences in host range within C. acuatum and other species of Colletotrichum (Du et al 2005;Guerber et al 2003;Liu et al 2012). If these and other genetic differences can be found to correspond directly to differences in host range, then this would support the thought that isolates of C. acutatum recovered from pepper are potentially distinct from isolates recovered from strawberry or citrus, and therefore pose less of a threat for cross-infection.…”

Section: Discussionmentioning

confidence: 99%

Management and cross-infectivity potential of Colletotrichum acutatum causing anthracnose on bell pepper in Florida

et al. 2013

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Early anthracnose caused by Colletotrichum acutatum has become an increasingly serious disease on green, unripe bell pepper fruit in Florida. This contrasts with earlier reports of anthracnose occurring on bell pepper primarily as a ripe-rot disease of mature, colored pepper fruit caused by Colletotrichum gloeosporioides. Management of anthracnose on green bell pepper fruit using fungicides and a commercial inducer of systemic acquired resistance, acibenzolar-S-methyl (ASM), was evaluated during three seasons. In two of the three trials, all the fungicides tested including azoxystrobin, fludioxonil + cyprodinil, mancozeb, famoxadone + cymoxanil, copper hydroxide, and ASM significantly increased the number of marketable fruit compared with control plants. These trials identified fungicides that could contribute to a successful pest management program on pepper for controlling anthracnose caused by C. acutatum. The cross-infectivity potential of C. acutatum was investigated on tomato and strawberry by in vitro and field inoculation. Anthracnose lesions formed readily on wound-inoculated detached fruits of all hosts in in vitro assays. Under field conditions, after inoculation, anthracnose lesions occurred on pepper fruit but no lesions of anthracnose were found on either ripe or unripe tomato or strawberry fruit in adjacent plots.

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“…Symptoms caused by C. acutatum are often referred to as anthracnose and they include fruit rot, flower blight, foliar blight, stem necrosis, abnormal growth, premature abscission of fruit and root rot (Peres et al, 2005;Sreenivasaprasad & Talhinhas, 2005). Although C. acutatum is generally viewed as a broad host range pathogen, there is considerable strain variation within C. acutatum, and one or a few strains are generally responsible for disease on each susceptible host (Guerber et al, 2003;MacKenzie et al, 2009;Sreenivasaprasad & Talhinhas, 2005). Therefore, strains within C. acutatum have a much more limited host range than would be suggested by the wide range of hosts infected by C. acutatum as a whole.…”

Section: Introductionmentioning

confidence: 99%

Colonization of citrus leaves and secondary conidiation response to citrus flower extracts by non-postbloom fruit drop strains of Colletotrichum acutatum

MacKenzie¹,

Peres²,

Timmer³

2010

Trop. plant pathol.

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Postbloom fruit drop (PFD) is caused by a unique strain of Colletotrichum acutatum that infects citrus flowers, producing necrotic lesions on petals and the abscission of fruitlets. This strain survives on vegetative tissues during non-flowering periods, and conidial production on colonized tissue is stimulated by flower extracts. Genetically distinct strains from other hosts can cause PFD symptoms, but their survival on vegetative tissues and ability to sporulate in response to flower extracts is unknown. Isolates from anthracnose-affected blueberry, leatherleaf fern, strawberry, Key lime and PFD-affected sweet orange were evaluated for their ability to survive on leaves after inoculation and to produce conidia in response to flower extracts. Viable propagules were recovered from leaves inoculated with all non-PFD isolates and recovery of some was equal to or greater than that of the PFD isolate. The number of propagules recovered increased for all isolates after treatment with flower extracts and was comparable across isolates. Application of sucrose to leaves inoculated with the PFD isolate increased the number of propagules recovered from leaves, suggesting that the response to flower extracts was nutritional. Non-PFD isolates of C. acutatum can colonize and reproduce on leaves, and their inability to cause epidemics is probably due to their reduced pathogenicity to flowers. Key words: etiology, anthracnose, blueberry, leatherleaf fern, strawberry. RESUMO Colonização de folhas de citros e formação de conídios secundários por isolados de Colletotrichum acutatum não causadores de podridão floral dos citrosA podridão floral dos citros (PFC), causada por Colletotrichum acutatum, infecta flores e produz lesões necróticas nas pétalas que induzem a abscisão de frutos jovens. O fungo sobrevive em tecidos vegetativos nos períodos entre floradas e a produção de conídios em tecidos colonizados é estimulada pela aplicação de extrato de flores. Isolados de C. acutatum de outros hospedeiros são geneticamente distintos e também podem causar sintomas de podridão floral, porém sua sobrevivência em tecidos vegetativos e sua habilidade de esporulação em resposta à aplicação de extrato de flores é desconhecida. Nesse trabalho, isolados de C. acutatum causadores de antracnose em mirtilo, samambaia-preta, morango e limão galego foram avaliados quanto à sua habilidade de sobrevivência e produção de conídios em resposta a aplicação de extrato de flores. Propágulos viáveis foram obtidos de folhas de citros inoculadas com isolados de todos os hospedeiros e a recuperação de alguns isolados foi igual ou maior do que a de isolados de PFC. O número de propágulos obtidos aumentou significativamente para todos os isolados após o tratamento com extrato de flores. A aplicação de sucrose em folhas inoculadas com um isolado de PFC também aumentou o número de propágulos obtidos, indicando que a resposta ao extrato de flores deve ser devido a nutrientes. Os resultados deste estudo indicam que isolados de C. acutatum de outros hospedeiros...

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Characterization of diversity inColletotrichum acutatumsensu latoby sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility

Cited by 244 publications

References 51 publications

Genetic variation among Phyllosticta strains isolated from citrus in Florida that are pathogenic or nonpathogenic to citrus

Genetic variation among Phyllosticta strains isolated from citrus in Florida that are pathogenic or nonpathogenic to citrus

Management and cross-infectivity potential of Colletotrichum acutatum causing anthracnose on bell pepper in Florida

Colonization of citrus leaves and secondary conidiation response to citrus flower extracts by non-postbloom fruit drop strains of Colletotrichum acutatum

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