Characterization of <i>Helicobacter felis</i> by Pulsed‐Field Gel Electrophoresis, Plasmid Profiling and Ribotyping

Jalava, Katri; Ungria, Maria Corazon A. De; O’Rourke, Jani; Lee, Adrian V.; Hirvi, Urszula; Hänninen, Marja‐Liisa

doi:10.1046/j.1523-5378.1999.09040.x

Cited by 8 publications

(1 citation statement)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ceelen et al (9) fingerprinted 16 isolates from two broiler flocks by AFLP and showed that isolates from each of the individual flocks examined clustered according to their flock of origin. Ribotyping with HaeIII successfully differentiated the intraspecies variation of both H. pylori and H. felis strains (7,17). However, no data on typeability and the discriminatory power of ribotyping for H. pullorum isolates have been reported yet.…”

mentioning

confidence: 99%

Prevalence ofHelicobacterpullorumin Conventional, Organic, and Free-Range Broilers and Typing of Isolates

Manfreda

Parisi²,

Lucchi

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

Helicobacter pullorum represents a potential food-borne pathogen, and avian species appear to be a relevant reservoir of this organism. In this study, the prevalence of H. pullorum was investigated at 30 conventional farms where 169 ceca from 34 flocks were tested, at eight organic farms where 39 ceca from eight flocks were tested, and at seven free-range farms where 40 ceca from eight flocks were tested. All of the ceca were obtained from healthy broiler chickens. Moreover, amplified fragment length polymorphism, pulsed-field gel electrophoresis, and automated ribotyping were employed to estimate the levels of genetic variability of H. pullorum broiler isolates within and between flocks. Overall, Gram-negative, slender, curved rods, identified as H. pullorum by PCR, were isolated at 93.3% of the farms tested. The percentage of positive free-range farms (54.2%) was significantly lower than that of conventional (100%) or organic (100%) farms (P < 0.001). The level of within-flock genetic variability, calculated as the number of flocks colonized by isolates genetically different by all of the typing methods, was 34.9%. Isolates showing identical profiles by each typing method were observed in 11.6% of the flocks, but they were never detected between flocks. However, groups of isolates clustered together with an overall similarity level of >85%. Our results suggest that even though a high level of genetic variability is attributable to H. pullorum broiler isolates, their hierarchical genotyping produces data useful for epidemiological investigations.

show abstract

mentioning

confidence: 99%

Prevalence ofHelicobacterpullorumin Conventional, Organic, and Free-Range Broilers and Typing of Isolates

Manfreda

Parisi²,

Lucchi

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

Helicobacter

On¹,

Lee²,

O’Rourke³

et al. 2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

He . li . co . bac ' ter. Gr. n. helix a spiral; M.L. masc. n. bacter a staff; M.L. masc. n. Helicobacter a spiral rod. Proteobacteria / Epsilonproteobacteria / Campylobacterales / Helicobacteraceae / Helicobacter Cells may be curved, spiral, or fusiform rods , 0.2–1.2 × 1.5–10.0 µm. Spiral cells may be tightly or loosely wound depending on the species, and on the age and condition of the culture examined. Cells in old cultures or those exposed to air become coccoid. Ultrastructural studies show that periplasmic fibers may be observed on the cell surface of a few taxa and an electrondense glycocalyx‐ or capsule‐like layer has been observed on the cell surface of some species. Nonsporeforming. Gram negative. Motile with a rapid corkscrew‐ or slower wave‐like motion due to flagellar activity. Multiple sheathed flagella are seen in most species , frequently with a bipolar distribution. Typical cell morphologies of various Helicobacter species are shown in Figs. BXII.ε.5 and BXII.ε.6. The mol % G + C of the DNA is : 24–48. Type species : Helicobacter pylori (Marshall, Royce, Annear, Goodwin, Pearman, Warren, Armstrong 1984) Goodwin, Armstrong, Chilvers, Peters, Collins, Sly, McConnell, Harper 1989a, 403 ( Campylobacter pylori Marshall, Royce, Annear, Goodwin, Pearman, Warren, Armstrong 1984, 87.)

show abstract