Characterization of steroidal saponins in crude extracts from Dioscorea zingiberensis C. H. Wright by ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry

Zhu, Jun‐Jie; Guo, Xiao; Fu, Shaoping; Zhang, Xiuli; Liang, Xinmiao

doi:10.1016/j.jpba.2010.05.019

Cited by 74 publications

(60 citation statements)

References 18 publications

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“…Quasi-molecular ions ([MÀH] À and [MþH] þ ) and adduct ions ([MþHCOO] À and [Mþ Na] þ ) were identified for most constituents. By comparing the retention times and accurate mass data of total saponins samples and reference standards solutions, as well as the widely accepted accuracy threshold for confirmation of elemental composition established at 5 ppm (Zhu et al, 2010), six characteristic peaks of glycosides were identified in both DNTS and DPTS sample solutions; these are PD (1), PG (2), PSD (3), DC (5), GR (6) and PV (7) (Fig. 3).…”

Section: Characterization Of the Main Constituents In Dnts Dpts And mentioning

confidence: 99%

“…The contents of PD and DC in DNTS and DPTS were remarkably higher than that in DZTS as their peak intensity indicated. By comparing molecular weights, retention times, MS/MS behavior and reported structures with compounds described in the literature Zhu et al, 2010), an additional nine glycosides (For full chemical names, see Fig. 2) were tentatively identified in DZTS as PAR (9) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39...…”

Section: Characterization Of the Main Constituents In Dnts Dpts And mentioning

confidence: 99%

“…Several phytochemical studies have been done on these three herbs in vitro (Li et al, 2006;Tang et al, 2013;Zhu et al, 2010), suggesting that the chemical composition of DN and DP are similar, and that both are quite different from DZ. But the questions of what the bioactive components are and how they prevent and treat cardiovascular disease have not yet been fully addressed.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

UPLC-QTOF-MS identification of metabolites in rat biosamples after oral administration of Dioscorea saponins: A comparative study

Tang

Pang

et al. 2015

Journal of Ethnopharmacology

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Section: Characterization Of the Main Constituents In Dnts Dpts And mentioning

confidence: 99%

Section: Characterization Of the Main Constituents In Dnts Dpts And mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

UPLC-QTOF-MS identification of metabolites in rat biosamples after oral administration of Dioscorea saponins: A comparative study

Tang

Pang

et al. 2015

Journal of Ethnopharmacology

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“…Wright (DZW), an important and widely used traditional Chinese medicine. As a widely distributed plant in China, DZW in nature have a variety of interesting biological activities and its rhizome has been used as a traditional Chinese medicine for anthrax, rheumatic heart disease, rheumatoid arthritis etc [6]. Steroidal saponins are identified as the major active components of DZW, including zingiberensis new saponin, diosgenin, trillin, gracillin [7][8].…”

Section: Deltonin (Diosgenin-3-o-β-d-glucopyranosyl (1→4)-[α-lrhamnopmentioning

confidence: 99%

Deltonin induced both apoptosis and autophagy in head and neck squamous carcinoma FaDu cell

Xie¹,

Fan²,

Jiang³

et al. 2015

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For decades, despite the advancement of medical science, the prognosis of head and neck squamous cell carcinoma (HNSCC), has not improved. Deltonin is one of the major active components of Dioscorea Zingiberensis Wright that has been used for anthrax, rheumatic heart disease, rheumatoid arthritis etc. By employing HNSCC FaDu cell and normal human epidermal keratinocyte, we investigate deltonin efficacy and associated mechanism in both cell culture and nude mice xenografts. Deltonin treatment selectively prevents proliferation of FaDu cells by cell-cycle arrest and induction of apoptosis, via activating checkpoint kinase Chk1and Chk2 as well as caspases 8, 9 and 3. Meanwhile, we found that treatment with deltonin induced autophagy, which played a protective role against deltonin-induced apoptosis. Further studies revealed that deltonin activated autophagy by Akt-mTOR signaling. Additionally, xenograft model showed that administration of deltonin significantly inhibited tumor growth and prolonged survival of tumor bearing mice. Our studies suggested that deltonin might be a potential chemotherapeutic agent against HNSCC, which might contribute to clinical application and pharmacological study of deltonin in future anti-cancer research.

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“…Its rhizome has been known as a traditional Chinese medicine for a long time and used as a folk treatment for cough, anthrax, rheumatic heart disease, rheum, arthritis, tumefaction and sprain [6]. Steroidal saponins are the major active components of DZW, including diosgenin, trillin, gracillin, diosgenin diglucoside protogracillin, deltonin, zingiberensis newsaponin and so on [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Deltonin, a Steroidal Saponin, Inhibits Colon Cancer Cell Growth <i>in Vitro</i> and Tumor Growth <i>in Vivo</i> via Induction of Apoptosis and Antiangiogenesis

Tong

Qing

Shu

et al. 2011

Cell Physiol Biochem

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Deltonin, a steroidal saponin, isolated from Dioscorea zingiberensis Wright (DZW), has shown high-cytotoxic activity in cancer cells. However, its mechanisms and in vivo anti-cancer effects remain unknown. In the present study, we evaluated the effects and explored the anti-tumor mechanisms of deltonin on a panel of colon cancer cell lines and in a mouse model of murine colon cancer C26. Deltonin had more cytotoxic effect on C26 cells than 5-fluorouracil had, promoting dramatic G2-M phase arrest and apoptosis in C26 cells in a concentration-dependent manner; oral administration of deltonin significantly inhibited the tumor growth and prolonged survival of the tumor bearing mice. The deltonin treatment caused a noticeable apoptosis in tumor tissue, which associated with increased levels of Bax, activated caspase-3, caspase-9, and cleaved poly (ADPribose) polymerase, decreased pro-caspase-8, pro-caspase-9, Bcl-2 expression levels and extracellular signal-regulated kinase-1/2 activity; and dose-dependently inhibit angiogenesis. In conclusion, the findings in this study demonstrated that deltonin is an effective natural agent for cancer therapy, which may be mediated, in part, by induction of apoptosis, as well as involve mitogen-activated protein kinase pathways, and inhibition of angiogenesis.

show abstract

Characterization of steroidal saponins in crude extracts from Dioscorea zingiberensis C. H. Wright by ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry

Cited by 74 publications

References 18 publications

UPLC-QTOF-MS identification of metabolites in rat biosamples after oral administration of Dioscorea saponins: A comparative study

UPLC-QTOF-MS identification of metabolites in rat biosamples after oral administration of Dioscorea saponins: A comparative study

Deltonin induced both apoptosis and autophagy in head and neck squamous carcinoma FaDu cell

Deltonin, a Steroidal Saponin, Inhibits Colon Cancer Cell Growth <i>in Vitro</i> and Tumor Growth <i>in Vivo</i> via Induction of Apoptosis and Antiangiogenesis

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