Characterization of the DNA of a Nonoccluded Baculovirus, Hz-1V

Huang, Yuan‐Shen; Hedberg, M.; Kawanishi, C.Y.

doi:10.1128/jvi.43.1.174-181.1982

Cited by 34 publications

(9 citation statements)

References 28 publications

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“…Hz-1 virus was identified by Granados and coworkers in 1978 (22) as a persistent infecting baculovirus agent in the IMC-Hz-1 ovarian cell line. It is an enveloped, nonoccluded, rod-shaped virus that contains a double-stranded circular 228-kb DNA genome (12,24). The virus particles are usually heterogeneous in length according to examination by electron microscopy.…”

mentioning

confidence: 99%

Persistent Hz-1 Virus Infection in Insect Cells: Evidence for Insertion of Viral DNA into Host Chromosomes and Viral Infection in a Latent Status

Lin

Lee

Chen

et al. 1999

J Virol

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Persistent/latent viral infections of insect cells are a prominent though poorly understood phenomenon. In this study, the long-term association between the Hz-1 virus and insect host cells, conventionally referred to as persistent viral infection, is described. With the aid of a newly developed fluorescent cell-labeling system, we found that productive viral replication occurs by spontaneous viral reactivation in fewer than 0.2% of persistently infected cell lines over a 5-day period. Once viral reactivation takes place, the host cell dies. The persistently infected cells contain various amounts of viral DNA, and, in an extreme case, up to 16% of the total DNA isolated from infected cells could be of viral origin. Both pulsed-field gel electrophoresis and in situ hybridization experiments showed that some of these viral DNA molecules are inserted into the host chromosomes but that the rest of viral DNA copies are free from host chromosomes. Thus, Hz-1 virus is the first nonretroviral insect virus known to insert its genome into the host chromosome during the infection process. These data also suggest that the previously described persistent infection of Hz-1 virus in insect cells should be more accurately referred to as latent viral infection.

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confidence: 99%

Persistent Hz-1 Virus Infection in Insect Cells: Evidence for Insertion of Viral DNA into Host Chromosomes and Viral Infection in a Latent Status

Lin

Lee

Chen

et al. 1999

J Virol

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“…It was isolated from persistently infected IMC-Hz-1 tissue culture cells by Granados and coworkers (9). The Hz-1 viral genome is a 228-kb covalently closed circular double-stranded DNA molecus (5,6,10). This virus can establish both persistent and productive infections in several lepidopteran insect cell lines.…”

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confidence: 99%

“…Defective virus particles containing genomic deletions have been observed in some Hz-1 viral isolates (5,6,10). Burand et al (5) showed that defective virus particles are generated following serial passage of the standard virus in vitro and are required for establishment of persistent infections.…”

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Differential expression of Hz-1 baculovirus genes during productive and persistent viral infections

Chao

Wood

Chang

et al. 1992

J Virol

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Hz-1 viral RNA transcription was studied during productive and persistent infections. The RNAs were localized to 10-to 30-kb regions within the viral genome, and the timing of their expression was determined. During productive infections, we detected 101 virus-specific transcripts that could be grouped into three categories by time of appearance. At 2 h postinoculation (p.i.), a total of 34 virus-specific transcripts were detected. An additional 51 and 16 virus-specific transcripts appeared between 4 and 6 h p.i. and at 8 h p.i., respectively. After 8 h, no new transcripts were found. Under conditions of persistent infection, we detected

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“…Hz-1 virus is rod-shaped, enveloped but with no occlusion body and its particle size is 414 ± 30 × 80 ± 30 nm (Burand et al , 1986). The viral genome of Hz-1 is 228 kb double-stranded circular DNA with a molecular weight of 1.31 × 10 7 Da (Huang et al , 1982). Hz-1 virus was previously classified as a member of Baculoviridae , and defined as the type species of the Nudibaculovirinae .…”

Section: Introductionmentioning

confidence: 99%

“…, 1986). The viral genome of Hz‐1 is 228 kb double‐stranded circular DNA with a molecular weight of 1.31 × 10 7 Da (Huang et al. , 1982).…”

Section: Introductionmentioning

confidence: 99%

The thymidylate synthase gene of Hz‐1 virus: A gene captured from its lepidopteran host

Chen

Tso

Yeh

et al. 2001

Insect Molecular Biology

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The sequence analysis of a thymidylate synthase gene was identified in the Hz-1 virus HindIII-D fragment. The viral thymidylate synthase gene encodes a protein of 295 amino acids, and is closely related to that of insect, mammals and herpesvirus. The thymidylate synthase gene identified was a genuine viral gene in that it was only detected in cells infected with Hz-1 virus but not in the mock infected cells, by Southern blot analysis and by RT-PCR. Results of phylogenetic analysis based on non-synonymous and amino acid distances suggested that the TS gene of Hz-1 virus was grouped closely with that of Bombyx mori. High bootstrap values confirmed that the thymidylate synthase of Hz-1 virus was acquired by a capture event from its lepidopteran host. Results of both sequence divergences and phylogenetic analysis suggested that TS genes in insect viruses, Hz-1, CIV, and MsEPV may have a different history or originated from different capture events.

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Characterization of the DNA of a Nonoccluded Baculovirus, Hz-1V

Cited by 34 publications

References 28 publications

Persistent Hz-1 Virus Infection in Insect Cells: Evidence for Insertion of Viral DNA into Host Chromosomes and Viral Infection in a Latent Status

Persistent Hz-1 Virus Infection in Insect Cells: Evidence for Insertion of Viral DNA into Host Chromosomes and Viral Infection in a Latent Status

Differential expression of Hz-1 baculovirus genes during productive and persistent viral infections

The thymidylate synthase gene of Hz‐1 virus: A gene captured from its lepidopteran host

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