Characterization of the gene encoding the plastid-located glutamine synthetase of Phaseolus vulgaris: regulation of ?-glucuronidase gene fusions in transgenic tobacco

Abstract

The gln-delta gene, which encodes the plastid-located glutamine synthetase of Phaseolus vulgaris, was cloned and its promoter region was sequenced. Primer extension analysis was used to map the two major transcription initiation sites which are about 90 nucleotides apart. A fusion of 2.3 kb of the upstream region of the gln-delta gene to the reporter gene uidA encoding beta-glucuronidase was shown to be expressed in the chlorophyllous cell types of leaves and stems and in the root meristem region of transgenic tobacco. Analysis of a series of three 5' promoter deletion fusions revealed the presence of a region essential for promoter activity between -786 and -327 and regions involved in tissue-specific regulation and light regulation between -786 and +43.

Light Regulation of Nitrate uptake, Assimilation and Metabolism

“…Light responsive GS2 promoters were demonstrated for Phaseolus vulgaris (Cock et al 1992) and pea (Tjaden et al 1995). The GS2 pea promoter contains an AT-rich 33 bp region at 807 bp upstream of the transcription start site, and when this AT-rich region was deleted, expression was reduced 10-fold (Tjaden and Coruzzi 1994).…”

cis Elements and trans-Acting Factors Affecting Regulation of a Nonphotosynthetic Light-Regulated Gene for Chloroplast Glutamine Synthetase

“…1). This TATA element corresponds in sequence and location to that determined for the Glnd gene (Cock et al, 1992). Deletion of this TATA sequence in 3'-deletion constructs revealed this to be a functional TATA element by in vivo analysis (construct GS2/ 3'-59 in Fig.…”

“…Vol. 108, 1995 expression of GlnS lie between -786 and +43 bp of the promoter (Cock et al, 1992). However, a shorter construct containing 327 bp of the GlnS promoter gave barely detectable levels of GUS activity and was not useful in further dissection of the regulatory cis elements.…”

“…Promoter studies have been performed on GInS (Cock et al, 1992). A construct containing 0.8 kb of the GInS promoter exhibited tissue-specific expression and light-regulated expression in seedlings of transgenic tobacco (Cock et al, 1992). A promoter-deletion analysis revealed that cis elements important to the tissue-specific and light-regulated Plant Physiol.…”

“…A detailed dissection of the promoter elements for the GS2 gene of P. sativum should uncover the basis for the complex transcriptional regulation of this gene, formerly thought to be a "housekeeping" gene. Promoter studies have been performed on GInS (Cock et al, 1992). A construct containing 0.8 kb of the GInS promoter exhibited tissue-specific expression and light-regulated expression in seedlings of transgenic tobacco (Cock et al, 1992).…”