Chemical Analysis of Single Cells

Trouillon, Raphaël; Passarelli, Melissa K.; Wang, Jun; Kurczy, Michael E.; Ewing, Andrew G.

doi:10.1021/ac303290s

Cited by 166 publications

(146 citation statements)

References 196 publications

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“…gene expression, protein activities, ion fluctuations, signaling) at the single cell level is key to understanding cellular behavior in a complex environment. [1][2][3][4] Nanoscale devices are ideal single-cell surgical tools because of their potential for high spatial and temporal resolution recordings with minimal disturbance to cell functions. 5,6 Recently, two groups independently developed cellular nanoendoscopes for single cell analysis.…”

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confidence: 99%

Electrochemical Nanoprobes for Single-Cell Analysis

et al. 2014

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The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microcopy probes which should allow high resolution electrochemical mapping of species on or in living cells.

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confidence: 99%

Electrochemical Nanoprobes for Single-Cell Analysis

et al. 2014

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“…Researchers must investigate the reactivity of a protein with its physiological partners to understand its role in cellular pathways; concentration measurements alone could be misleading, because the local environment of a protein can drastically alter its activity. Emerging capillary micro-/nanofabrication technologies allow the chemical reactivity of the target species to be investigated (3,(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). Allbritton and coworkers (11,12) embarked on the development of this technology and used capillary electrophoresis to analyze intracellular kinase activity.…”

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confidence: 99%

Nanokit for single-cell electrochemical analyses

Pan

Jiang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

115

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The development of more intricate devices for the analysis of small molecules and protein activity in single cells would advance our knowledge of cellular heterogeneity and signaling cascades. Therefore, in this study, a nanokit was produced by filling a nanometersized capillary with a ring electrode at the tip with components from traditional kits, which could be egressed outside the capillary by electrochemical pumping. At the tip, femtoliter amounts of the kit components were reacted with the analyte to generate hydrogen peroxide for the electrochemical measurement by the ring electrode. Taking advantage of the nanotip and small volume injection, the nanokit was easily inserted into a single cell to determine the intracellular glucose levels and sphingomyelinase (SMase) activity, which had rarely been achieved. High cellular heterogeneities of these two molecules were observed, showing the significance of the nanokit. Compared with the current methods that use a complicated structural design or surface functionalization for the recognition of the analytes, the nanokit has adapted features of the well-established kits and integrated the kit components and detector in one nanometer-sized capillary, which provides a specific device to characterize the reactivity and concentrations of cellular compounds in single cells.

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“…Due to cellular heterogeneity within an isogenic cell population, individual analysis of cells will lead to a more sensitive representation of cell-to-cell variations, instead of a stochastic average analysis by bulk measurements. [24][25][26][27][28][29] Classical ICP-MS analysis of cells is the bulk analysis of large amounts of cells with a lysis, extraction or digestion; 30 this is an approach that is hardly adapted for evaluating any cell-to-cell variance. Time-resolved measurement via a direct cell introduction method is a relatively recent method for individual analysis of cells by ICP-MS, [14][15][16][17][18][19][20][21][22][23] an approach that can detect the elements in individual cells with a high sensitivity, often called "single particle mode".…”

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confidence: 99%