Chemical Composition and Hepatoprotective Effects of Polyphenol-Rich Extract from Houttuynia cordata Tea

Abstract

This study was designed to investigate the antioxidant activity, hepatoprotective effect, and phenolic composition of the ethyl acetate fraction (EAF) extracted from Houttuynia cordata tea. EAF was shown to exhibit strong ferric-reducing antioxidant power (FRAP) and scavenging activity against DPPH radical in vitro, and the antioxidant effects were further verified by suppressing CCl₄-induced oxidative stress in mouse liver at three tested doses of EAF (250, 500, and 1000 mg/kg bw). Pretreatment with EAF (1000 mg/kg bw) prior to CCl₄ administration significantly (p < 0.001) decreased the CCl₄-elevated levels of serum AST, ALT, alkaline phosphatase, total bilirubin, and hepatic MDA in mice and prevented the increases in GSH, SOD, and CAT caused by CCl₄. HPLC analysis revealed that three predominantly polyphenolic compounds present in EAF were quercitrin (111.7 μg/mg), quercetin (43.8 μg/mg), and hyperoside (29.1 μg/mg). These results combined with liver histopathology indicate that EAF possesses a significant protective effect against acute hepatotoxicity induced by CCl₄, which may be due to the strong antioxidant activity of phenolic components.

Antioxidant activity of herbaceous plant extracts protect against hydrogenperoxide-induced DNA damage in human lymphocytes

“…Our present study demonstrates that, among the six investigated species, the higher content of polyphenols, flavonols, and antioxidant properties in HC and MA plants may be the reason for their wide medicinal use. Both species can be used as potent medicinal herbs for novel bioactive compounds with high free radical scavenging activity, and extracts of these plants may been attractive alternative for managing oxidative stress-induced liver injury and drug-induced gastric ulcer [45,46]. Recently, Gargouri et al .…”

An ethyl acetate fraction derived from Houttuynia cordata extract inhibits the production of inflammatory markers by suppressing NF-кB and MAPK activation in lipopolysaccharide-stimulated RAW 264.7 macrophages

“…Yet another study revealed that quercitrin markedly overturned NO production in RAW 264.7 cells and inhibited the progression of inflammation through down-regulation of the NF-κB pathway [36,37]. Furthermore, Tian et al [15] reported that an EA fraction derived from H. cordata was rich in the polyphenolic compounds quercitrin, quercetin, and hyperoside, which were responsible for the antioxidant and hepatoprotective merits of the tea. In agreement with these previous studies, our results indicate that the ability of HCE-EA to suppress production of inflammatory mediators might be attributable to its content of active components, in particular chlorogenic acid, hyperoside, and quercitrin.…”

“…(family Saururaceae) is a perennial food plant widely distributed throughout Southeastern Asia. This plant is a leaf vegetable that is used as a herbal tea, in salads, or cooked with other vegetables [15]. Also, It has been used in traditional medicine for treatment of suppuration, chronic bronchitis and pneumonia, otitis, and cystitis [16,17].…”

“…Recent reports have demonstrated that the volatile oil and supercritical extract constituents of H. cordata are acutely important for the mitigation of inflammation [20,23,24]. Another recent study demonstrated that the ethyl acetate fraction of a H. cordata extract exerted a protective effect against chemokine (C-C motif) ligand 4 (CCL4)-induced acute hepatotoxicity in mice [15]. …”

Inhibitory effects of polyphenol-enriched extract from Ziyang tea against human breast cancer MCF-7 cells through reactive oxygen species-dependent mitochondria molecular mechanism

“…As far as we know, it is the first time that the antiproliferative properties of the polyphenolic extract derived from selenium-enriched Ziyang tea against human breast cancer MCF-7 cells have been evaluated. [18,19], 50 g of dried tea leaves were thoroughly mixed with 500 mL of 80% ethanol, and were extracted three times at 70 C for 60 minutes for each extraction while being vigorously shaken. The obtained supernatant was further concentrated and precipitated by 1.0M ZnCl 2 (pH 6.2), and 0.4M hydrochloric acid was used to dissolve the sediments.…”