Chromatographic fingerprint analysis of Fructus Aurantii Immaturus by HPLC-DAD and chemometric methods

Abstract

An efficient method for quality control of Fructus Aurantii Immaturus (FAI), a famous traditional Chinese medicine (TCM) was established. A simple and reliable high-performance liquid chromatography-photodiode array detector (HPLC-DAD) procedure coupled with chemometric methods was developed for fingerprint analysis, qualitative analysis and quantitative determination of this herb. In qualitative and quantitative analyses, heuristic evolving latent projection (HELP) method was employed to resolve the overlapping peaks of the tested samples. Two bioactive components, namely hesperidin and naringin, are confirmed and determined, together with four flavonoids compounds tentatively identified including two new ones. From fingerprint analysis, the fingerprint data were processed with correlation coefficients for quantitative expression of their similarity and dissimilarity. The developed method based on an integration of chromatographic fingerprint and quantitative analysis is scientific, and the obtained results can be applied to the quality control of herb medicine.

Fingerprint Analysis and Multi-ingredient Determination Using a Single Reference Standard for Saposhnikoviae Radix

“…Of the total 15 common peaks, 12 were identified by the hyphenated chromatographic techniques HPLC-MS/MS. The components are: GC (1), C (2), GV (3), 5-O-methylvisamminol (6), GH (7), psoralen (8), xanthotoxin (9), hamaudol (10), divaricatol (11), bergapten (12), 3′-O-acetyhamaudol (13), and 3′-O-angeloyhamaudol (15). Among them, compounds 1, 2, 3, 7, 8, 9 and 12 were unambiguously verified with the chemical reference standards.…”

“…[15][16][17][18] The identity, consistency and authenticity of samples can be determined by comparison of their chromatographic fingerprints using stoichiometry, such as similarity analysis, principal component analysis (PCA) and cluster analysis. 19,20 For more comprehensive quality control of TCM, simultaneous quantification of multiple ingredients also is essential.…”

Variations in chemical fingerprints and major flavonoid contents from the leaves of thirty‐one accessions of Hibiscus sabdariffa L.

“…The other is chemical fingerprint analysis, which has been accepted by World Health Organization (WHO) (Kong et al, 2009;World Health Organization, 1991). At present, fingerprint analysis, based on multivariate statistical analysis, such as similarity analysis (SA) and principal component analysis (PCA), is widely applied to discriminate the medicinal plants (Tian et al, 2009;Xu et al, 2011), and fruits (Sârbu et al, 2012).…”

Simultaneous quantitative determination of 20 active components in the traditional Chinese medicine formula Zhi‐Zi‐Da‐Huang decoction by liquid chromatography coupled with mass spectrometry: application to study the chemical composition variations in different combinations

“…In our previous study, the ultra-fast liquid chromatographic fingerprint of ZZDHD was established and the correlation between ZZDHD and its component herbs was studied (Tang et al, 2014). Despite many reports on component determination of the single herbs contained in ZZDHD (He et al, 2006;Bergonzi et al, 2012;Lu et al, 2011;Xu et al, 2011;Liu et al, 2012), there have been few related reports about simultaneous quantification of active components in ZZDHD, in which the selected marker chemicals did not cover all of the four component herbs, and high-performance liquid chromatography coupled with ultraviolet (UVD)/fluorescence detection (FLD) was adopted for the analysis Yao et al, 2013). The commonly used UVD or FLD has no way of analyzing chemicals with poor ultraviolet or fluorescent absorbance, which limits the detection accuracy (Zhang et al, 2013).…”