Chromatographic separation of racemic praziquantel and its residual determination in perch by LC-MS/MS

He, Lili; Gao, Fuming; Li, Erfen; Lee, Jauh Tzuoh; Bian, Linkan; Armstrong, Daniel W.

doi:10.1016/j.talanta.2017.05.026

Cited by 18 publications

(24 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Raw catfish muscle samples were purchased from a local market and were found to be negative for nitrofurans or their metabolites by LC-MS. The method used for sample processing has been described elsewhere (He et al, 2017). Catfish muscle was minced and hydrolysed using 1 M HCl, and 2-NBA was added and used for the derivatization reagent and the sample was extracted three times with ethyl acetate.…”

Section: Sample Pretreatmentmentioning

confidence: 99%

Development of a gold immunochromatographic strip for the rapid detection of 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in catfish

Lü

Song

et al. 2020

Food and Agricultural Immunology

View full text Add to dashboard Cite

In this study, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a gold immunochromatographic assay were developed to detect the furaltadone metabolite, 3-amino-5morpholinomethyl-2-oxazolidinone (AMOZ). The detection of AMOZ was based on the AMOZ derivative 3-([2-nitrophenyl] methyleneamino)-5-morpholinomethyl-2-oxazolidinone (2-NPAMOZ). The monoclonal anti-2-NPAMOZ antibody 4G11, was prepared and exhibited a 50%-inhibitory concentration (IC 50) of 0.049 ng/mL. Its cross-reactivity with structurally related analogues was less than 3%. This immunochromatographic assay was assembled for the rapid screening of AMOZ in catfish samples. The strip assay had a cutoff value of 0.1 μg/kg and a limit of detection (LOD) of 0.009 μg/kg. Analysis of AMOZ in catfish indicated that the results from the strip assay were highly comparable to results obtained using ic-ELISA and LC-MS/MS. Therefore, the colloidal gold (CG) immunoassay is a sensitive screening method for the detection of AMOZ residues in catfish samples.

show abstract

Section: Sample Pretreatmentmentioning

confidence: 99%

Development of a gold immunochromatographic strip for the rapid detection of 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in catfish

Lü

Song

et al. 2020

Food and Agricultural Immunology

View full text Add to dashboard Cite

show abstract

“…The residue was extracted by adding 1 mL of ethyl acetate, and the two supernatants were pooled and dried at 40°C under warm nitrogen. The residual material was dissolved in 1 mL of 0.5% PBST for analysis (L. He et al, 2017).…”

Section: Pzq Recovery By Ic-elisamentioning

confidence: 99%

“…PZQ detection methods are based on instrumental analysis such as spectroscopic methods (Piantavini, Pontes, Weiss, Sena, & Pontarolo, 2015), mass spectrometry, and chromatography (He et al, 2017;Yan, 2016;Zheng, Shao, & Jiang, 2016;Zrncic et al, 2014). Due to cost, cumbersome sample preparation steps, and large amount of manpower and material resources, these detection methods are not suitable for the detection of PZQ in aquatic products.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips

Shen

Liu

et al. 2019

Food and Agricultural Immunology

View full text Add to dashboard Cite

Praziquantel (PZQ) is a broad-spectrum antiparasitic drug used in mammals and fish. In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of PZQ residues. The hapten PZQ-HS produced by introducing an amino group at a benzene ring synthesized the immunogen and the coating antigen with carrier proteins. Highly sensitive monoclonal antibodies (mAbs) were prepared following mouse immunization and cell fusion. Under optimum conditions, the half maximal inhibitory concentration of the cell line 4E9 was 7.4 ng/mL, and the working range was 2.4-22.8 ng/mL. Immunochromatographic strips (ICS) were developed for the rapid detection of PZQ residues in mackerel. The reliability of the ICS assay was verified with PZQ-negative mackerel and confirmed by HPLC/MS. The cutoff value for ICS was 50 ng/mL PZQ. Our findings revealed that ic-ELISA and ICS can be used for the rapid detection of PZQ in mackerel.

show abstract

“…In the literature, there are many studies describing PZQ analysis in different matrices [4][5][6]. Moreover, several studies report the quantification of PZQ in food [7][8][9][10][11][12], but no study describes the determination of PZQ in gilthead sea bream (Sparus aurata L.) after in-feed treatment.…”

Section: Introductionmentioning

confidence: 99%

Determination of Praziquantel in Sparus aurata L. after Administration of Medicated Animal Feed

Baralla

Varoni

Nieddu

et al. 2020

Animals

View full text Add to dashboard Cite

Praziquantel (PZQ) is an anthelmintic drug used in humans and animals against Platyhelminthes and in aquaculture in the Far East. Medicated feed is one of the most convenient forms of oral administration of drugs in aquaculture because it allows to treat a large population of fish in an easy way. However, this treatment may lead to residues in fish intended for human consumption. In this study, a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed in order to verify the presence of PZQ in samples of Sparus aurata after oral administration of feed treated with PZQ. The method was validated according to international guidelines. It showed good recoveries, selectivity and sensitivity (LOD and LOQ were 3.0 and 9.3 ng/g, respectively), with precision and matrix effect values ≤ 15%. This method could also be applied to determine PZQ residue in other fish species and thus to evaluate the appropriate withdrawal time in treated fish intended for human consumption.

show abstract

Chromatographic separation of racemic praziquantel and its residual determination in perch by LC-MS/MS

Cited by 18 publications

References 30 publications

Development of a gold immunochromatographic strip for the rapid detection of 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in catfish

Development of a gold immunochromatographic strip for the rapid detection of 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in catfish

Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips

Determination of Praziquantel in Sparus aurata L. after Administration of Medicated Animal Feed

Contact Info

Product

Resources

About