Chromoplasts of Tomato Fruits. II. The Red Tomato

Harris, William M.; Spurr, Arthur R.

doi:10.2307/2440813

Cited by 100 publications

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“…7). The observation that the plastoglobule proteins underwent no changes during the transition from chloroplasts to chromoplasts is in line with the fact that the bulk of the carotenoids of tomato fruit are stored predominantly in the form of lycopene crystalloids in membrane-shaped structures (Harris and Spurr, 1969).…”

Section: Changes In Subplastidial Compartmentationsupporting

confidence: 60%

Proteomic Analysis of Chloroplast-to-Chromoplast Transition in Tomato Reveals Metabolic Shifts Coupled with Disrupted Thylakoid Biogenesis Machinery and Elevated Energy-Production Components

et al. 2012

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A comparative proteomic approach was performed to identify differentially expressed proteins in plastids at three stages of tomato (Solanum lycopersicum) fruit ripening (mature-green, breaker, red). Stringent curation and processing of the data from three independent replicates identified 1,932 proteins among which 1,529 were quantified by spectral counting. The quantification procedures have been subsequently validated by immunoblot analysis of six proteins representative of distinct metabolic or regulatory pathways. Among the main features of the chloroplast-to-chromoplast transition revealed by the study, chromoplastogenesis appears to be associated with major metabolic shifts: (1) strong decrease in abundance of proteins of light reactions (photosynthesis, Calvin cycle, photorespiration) and carbohydrate metabolism (starch synthesis/degradation), mostly between breaker and red stages and (2) increase in terpenoid biosynthesis (including carotenoids) and stress-response proteins (ascorbate-glutathione cycle, abiotic stress, redox, heat shock). These metabolic shifts are preceded by the accumulation of plastid-encoded acetyl Coenzyme A carboxylase D proteins accounting for the generation of a storage matrix that will accumulate carotenoids. Of particular note is the high abundance of proteins involved in providing energy and in metabolites import. Structural differentiation of the chromoplast is characterized by a sharp and continuous decrease of thylakoid proteins whereas envelope and stroma proteins remain remarkably stable. This is coincident with the disruption of the machinery for thylakoids and photosystem biogenesis (vesicular trafficking, provision of material for thylakoid biosynthesis, photosystems assembly) and the loss of the plastid division machinery. Altogether, the data provide new insights on the chromoplast differentiation process while enriching our knowledge of the plant plastid proteome.

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Section: Changes In Subplastidial Compartmentationsupporting

confidence: 60%

Proteomic Analysis of Chloroplast-to-Chromoplast Transition in Tomato Reveals Metabolic Shifts Coupled with Disrupted Thylakoid Biogenesis Machinery and Elevated Energy-Production Components

et al. 2012

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“…Since key biosynthetic enzymes are targeted to plastoglobules (Ytterberg et al, 2006;Schweiggert et al, 2011;Davidi et al, 2015), carotenoid biosynthesis is supposed to be initiated in these globules. Additionally, the undulating lines in Figure 11C can also been interpreted as early carotenoid depositions in tomato (Harris and Spurr, 1969). In parallel with carotenoid accumulation, tubular structures apparently developed independently of the globular structure, resulting in the formation of tubular-globular chromoplasts (Fig.…”

Section: Discussion Two Types Of Putative Amyloplast-to-chromoplast Tmentioning

confidence: 91%

“…The relationship between the architecture and carotenoid composition has been well stated in diverse pepper (Capsicum annuum) and tomato (Solanum lycopersicum) fruits (Kilcrease et al, 2013;Nogueira et al, 2013). Crystalline bodies have been observed in carrot (Daucus carota;Frey-Wyssling and Schwegler, 1965) and tomato (Harris and Spurr, 1969), which predominantly consist of b-carotene and lycopene, respectively. Globular and/or tubularglobular chromoplasts, in which numerous lipid droplets (also named plastoglobules), which act as passive storage compartments for triglycerides, sterol ester, and some pigments, are accumulated, were described for yellow fruits from kiwi (Actinidia deliciosa), papaya (Carica papaya), and mango (Mangifera indica), which contain lutein, b-cryptoxanthin, and b-carotene as the major pigments, respectively (Vasquez-Caicedo et al, 2006;Montefiori et al, 2009;Schweiggert et al, 2011).…”

mentioning

confidence: 96%

A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

Zeng

Wang

et al. 2015

Plant Physiol.

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(S.X.).Globular and crystalloid chromoplasts were observed to be region specifically formed in sweet orange (Citrus sinensis) flesh and converted from amyloplasts during fruit maturation, which was associated with the composition of specific carotenoids and the expression of carotenogenic genes. Subsequent isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analyses of purified plastids from the flesh during chromoplast differentiation and senescence identified 1,386 putative plastid-localized proteins, 1,016 of which were quantified by spectral counting. The iTRAQ values reflecting the expression abundance of three identified proteins were validated by immunoblotting. Based on iTRAQ data, chromoplastogenesis appeared to be associated with three major protein expression patterns: (1) marked decrease in abundance of the proteins participating in the translation machinery through ribosome assembly; (2) increase in abundance of the proteins involved in terpenoid biosynthesis (including carotenoids), stress responses (redox, ascorbate, and glutathione), and development; and (3) maintenance of the proteins for signaling and DNA and RNA. Interestingly, a strong increase in abundance of several plastoglobule-localized proteins coincided with the formation of plastoglobules in the chromoplast. The proteomic data also showed that stable functioning of protein import, suppression of ribosome assembly, and accumulation of chromoplast proteases are correlated with the amyloplast-to-chromoplast transition; thus, these processes may play a collective role in chromoplast biogenesis and differentiation. By contrast, the chromoplast senescence process was inferred to be associated with significant increases in stress response and energy supply. In conclusion, this comprehensive proteomic study identified many potentially new plastid-localized proteins and provides insights into the potential developmental and molecular mechanisms underlying chromoplast biogenesis, differentiation, and senescence in sweet orange flesh.

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“…Tomato fruit is a rich source of ascorbic acid (vitamin C). The red color of tomatoes is a result of the degradation of chlorophylls and the increased biosynthesis of carotenoids, thus it is related to the degree of maturity and postharvest life (Harris and Spurr, 1969).The nutritional significance of lycopene, a carotenoid with potent antioxidant activity has been reported and accumulating evidence has shown an inverse correlation between the consumption of tomato products rich in lycopene and the risk of several types of cancer and cardiovascular disease (Rao and Agarwal, 1999;Giovannucci, 2005;Talvas et al, 2010).Red chilli (Capsicum annuum) belongs to the genus Capsicum under Solanaceae family. Chilli besides imparting pungency and red color to the dishes, it is rich source of vitamin A, C and E and assists in digestion.…”

Section: Issn: 2319-7706 Volume 6 Number 8 (2017) Pp 3537-3545mentioning

confidence: 99%

Studies on Effect of Fermentation on Physicochemical Properties of Vegetables and Preparation of Sauce

Thorat¹,

Sadawarte²,

Sawate³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Chromoplasts of Tomato Fruits. II. The Red Tomato

Cited by 100 publications

References 0 publications

Proteomic Analysis of Chloroplast-to-Chromoplast Transition in Tomato Reveals Metabolic Shifts Coupled with Disrupted Thylakoid Biogenesis Machinery and Elevated Energy-Production Components

Proteomic Analysis of Chloroplast-to-Chromoplast Transition in Tomato Reveals Metabolic Shifts Coupled with Disrupted Thylakoid Biogenesis Machinery and Elevated Energy-Production Components

A Comprehensive Analysis of Chromoplast Differentiation Reveals Complex Protein Changes Associated with Plastoglobule Biogenesis and Remodeling of Protein Systems in Sweet Orange Flesh

Studies on Effect of Fermentation on Physicochemical Properties of Vegetables and Preparation of Sauce

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