Cinnamtannin B-1 Regulates Cell Proliferation of Spinal Cord Astrocytes and Protects the Cell from Oxygen-Glucose-Serum Deprivation/Reoxygenation-Induced Apoptosis

Abstract

Astrocytes are important for protecting neurons in the central nervous system. It has been reported that some antioxidants could protect astrocytes from ischemia/reperfusion-induced dysfunction. Cinnamtannin B-1 is a naturally occurring A-type proanthocyanidin that exhibits anti-oxidant properties. In this study, we investigated the effects of cinnamtannin B-1 on spinal cord astrocytes. Astrocytes were subjected to oxygen-glucose-serum deprivation for eight hours followed by reoxygenation with or without cinnamtannin B-1. We found that cinnamtannin B-1 protected astrocytes from oxygen-glucose-serum deprivation and reoxygenation-induced apoptosis. Concurrently, cinnamtannin B-1 promoted the proliferation of astrocytes whereas the extracellular regulated protein kinase (ERK) inhibitor reversed this effect. The results indicated that cinnamtannin B-1 protects astrocytes from oxygen-glucose-serum deprivation/reoxygenation-induced apoptosis by promoting astrocyte proliferation via an ERK pathway. Therefore, as an anti-oxidant, cinnamtannin B-1 might provide extra benefit for astrocyte protection during ischemia/reperfusion in the central nervous system.

DRAM1 Protects Neuroblastoma Cells from Oxygen-Glucose Deprivation/Reperfusion-Induced Injury via Autophagy

“…The OGD and reperfusion model was established as described previously [ 42 ]. DMEM medium was removed, and the Neuro-2a cells were washed twice with oxygen and glucose-free Hank’s Balanced Salt Solution (HBSS, pH 7.4, Gibco, Grand Island, NY, USA).…”

Recruitment of Mesenchymal Stem Cells to Damaged Sites by Plant-Derived Components

“…Cinnamtannin B-1 is a proanthocyanidin found in specific plants such as Cinnamomum zeylanicum (Sánchez-Rubio et al, 2018). Cinnamtannin B-1 exhibits anti-inflammatory, anti-oxidant and anti-thrombotic properties (López et al, 2008), and also protects neurons from ischemia/reperfusion-induced dysfunction (Chi et al, 2013), while inhibiting survival of cancer cells (Carriere et al, 2018), and protecting pancreatic acinar cells during pancreatitis (Rivera-Barreno et al, 2010).…”

The Toxicity of Mauli Banana (Musa Acuminata) Stem Water Extract on Bone Marrow Mesenchymal Stem Cell in Vitro

“…The increased ROS activity can activate p53, so cell apoptosis can occur [18]. Tannin also is known for its property to increase Erk, which activates p53 for the apoptosis pathway through elevation of transcription among certain proapoptotic Bcl families, especially Bax [19,20]. Other than the difference in solvent choice, the difference in Mauli banana stem extract dosages also showed different toxicity results toward MSC in vitro.…”

“…Mauli banana stem extract has the highest bioactive content of tannin (67.59%) [7]. Mauli banana has the proanthocyanidin type of tannin (condensed tannin) [22], which can induce tyrosine phosphorylation in tyrosine kinase insulin receptors on the surface of MSCs to activate the MAPK/Erk and phosphoinositide 3-kinase (PI3K)/Akt/mTOR pathways [20,21,23,24]. Based on the study of Haston et al [25], pERK1/2 staining can be detected in the marginal zone of Sox2+ stem cells, which shows that MAPK/Erk has a role in stem cell proliferation.…”