Circulating miR-3135b and miR-107 are potential biomarkers for severe hypertension

Shi, Jingwei; Ren, Yaxuan; Liu, Yunkai; Yi, Cheng; Liu, Yawen

doi:10.1038/s41371-020-0338-0

Cited by 14 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, miR-3656/EMT axis is required for gemcitabine-resistant in pancreatic cancer [13]. Previously, we proved that miR-3656 reduces NO generation and vWF cleavage, thereby retarding blood flow and potentially increasing blood pressure in hypertension [15,20]. In this paper, we established that miR-3656 injured endothelial cells by downregulating TFAP2C expression; furthermore, downregulated TFAP2C decreased KLF10 expression by binding to KLF10 promoter region.…”

Section: Discussionmentioning

confidence: 71%

Transcription factor AP-2 gamma/Krüppel-like factor 10 axis is involved in miR-3656-related dysfunction of endothelial cells in hypertension

Ren

Shi

Liu

et al. 2023

Journal of Hypertension

Self Cite

View full text Add to dashboard Cite

Background: Dysfunction of endothelial cells links to microvascular rarefaction, reflecting the pathogenesis of hypertension. Our previous studies found that miR-3656 reduces nitric oxide generation and von Willebrand factor (vWF) cleavage, thereby retarding blood flow and potentially increasing blood pressure. In this paper, we investigated mechanism of transcription regulation contributing to miR-3656-damaged endothelial cells in hypertension. Methods:The effects of miR-3656 on function of endothelial cells were analyzed on the basis of proliferation, migration, tube formation, and apoptosis. The mRNA level and protein level of genes were examined using quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Dual-luciferase reporter assay was performed to confirm the binding between miR-3656 and 3' untranslated region (UTR) of transcription factor AP-2 gamma (TFAP2C). The binding between TFAP2C and the promoter region of Krppel-like factor 10 (KLF10) was confirmed by chromatin immunoprecipitation-qPCR assay.Results: miR-3656 impaired the cell proliferation, migration, tube formation, and apoptosis of endothelial cells. miR-3656 inhibited the expression of TFAP2C by directly targeting 3'UTR of TFAP2C; moreover, miR-3656induced injury of endothelial cells was rescued by TFAP2C overexpression. Furthermore, downregulated TFAP2C decreased KLF10 expression by binding to KLF10 promoter region, and upregulated KLF10 reversed the effects of silencing TFAP2C on endothelial cells. These inhibitory processes led to interference of miR-3656 to KLF10promoted function of endothelial cells. Conclusion:TFAP2C/KLF10 axis is involved in miR-3656related dysfunction of endothelial cells in hypertension. The 3'UTR of TFAP2C and KLF10 promoter region are the hubs of the TFAP2C/KLF10 axis.

show abstract

Section: Discussionmentioning

confidence: 71%

Transcription factor AP-2 gamma/Krüppel-like factor 10 axis is involved in miR-3656-related dysfunction of endothelial cells in hypertension

Ren

Shi

Liu

et al. 2023

Journal of Hypertension

Self Cite

View full text Add to dashboard Cite

show abstract

“…Confirming the potential targets and their effects/pathways found by our miRDB and the GO analysis, our literature research yielded the first hints of their involvement in hemorheological abnormalities such as hypertension/coronary artery calcification ( hsa -miR-3135b), pulmonary arterial hypertension ( hsa -miR-584-5p), angiogenesis/hypoxia ( hsa -miR-12136), and damaged vascular smooth muscles ( hsa -miR-550a-3p) [ 41 , 42 , 43 , 44 , 45 , 46 ]. Since the control group was lighter than the patient group (72.28 ± 8.83 kg vs. 85.51 ± 16.87 kg), the hsa -miR-584-5p and hsa -miR-550a-3p results may be due to this difference, as both miRNAs have also been linked to obesity/insulin resistance [ 47 , 48 ].…”

Section: Discussionmentioning

confidence: 91%

Differential Expression of microRNAs in Serum of Patients with Chronic Painful Polyneuropathy and Healthy Age-Matched Controls

et al. 2023

View full text Add to dashboard Cite

Polyneuropathies (PNP) are the most common type of disorder of the peripheral nervous system in adults. However, information on microRNA expression in PNP is lacking. Following microRNA sequencing, we compared the expression of microRNAs in the serum of patients experiencing chronic painful PNP with healthy age-matched controls. We have been able to identify four microRNAs (hsa-miR-3135b, hsa-miR-584-5p, hsa-miR-12136, and hsa-miR-550a-3p) that provide possible molecular links between degenerative processes, blood flow regulation, and signal transduction, that eventually lead to PNP. In addition, these microRNAs are discussed regarding the targeting of proteins that are involved in high blood flow/pressure and neural activity dysregulations/disbalances, presumably resulting in PNP-typical symptoms such as chronical numbness/pain. Within our study, we have identified four microRNAs that may serve as potential novel biomarkers of chronic painful PNP, and that may potentially bear therapeutic implications.

show abstract

“…Kim et al reported that genetic variant of CHRM3 with high genetic risk scores was associated with impaired insulin sensitivity in PCOS patients 31 . Shi et al suggested that miR‐3135b was a potential biomarker for severe hypertension 32 . Besides, differently expressed hsa‐miR‐3135b in PCOS has been reported previously 33,34 .…”

Section: Discussionmentioning

confidence: 92%

Identification of critical miRNAs and mRNAs associated with polycystic ovary syndrome

Diao

Yao

Wang

et al. 2021

J of Obstet and Gynaecol

View full text Add to dashboard Cite

Aim Polycystic ovary syndrome (PCOS) is a complicated endocrine and metabolic abnormality diseases common in women of child‐bearing age. This study aims to screen out critical miRNAs and mRNAs associated with PCOS, which may be conducive to offer novel insights and treatment for the diseases. Methods Three mRNA datasets and one miRNA dataset derived from granulosa cells of patients with PCOS and normal controls were downloaded to obtain the differentially expressed mRNAs (DEmRNAs) and miRNAs (DEmiRNAs). Then, DEmiRNA‐target DEmRNAs analysis and functional annotation of DEmiRNA‐target DEmRNAs were performed. Quantitative real time polymerase chain reaction (qRT‐PCR) validation of the expression of the selected DEmRNAs and DEmiRNAs were performed. Results A total of 1643 DEmRNAs, 88 DEmiRNAs, 2406 DEmiRNA (down)‐DEmRNA (up), and 2179 DEmiRNA (up)‐DEmRNA (down) pairs were obtained. The functional annotation of DEmiRNA‐target DEmRNAs revealed that C‐type lectin receptor signaling pathway, Steroid biosynthesis and Galactose metabolism were significantly enriched KEGG pathways. Conclusion These findings may provide make contribution to understanding PCOS pathogenesis, diagnosis, or treatment.

show abstract

Circulating miR-3135b and miR-107 are potential biomarkers for severe hypertension

Cited by 14 publications

References 35 publications

Transcription factor AP-2 gamma/Krüppel-like factor 10 axis is involved in miR-3656-related dysfunction of endothelial cells in hypertension

Transcription factor AP-2 gamma/Krüppel-like factor 10 axis is involved in miR-3656-related dysfunction of endothelial cells in hypertension

Differential Expression of microRNAs in Serum of Patients with Chronic Painful Polyneuropathy and Healthy Age-Matched Controls

Identification of critical miRNAs and mRNAs associated with polycystic ovary syndrome

Contact Info

Product

Resources

About