Citrus Pectin:  Characterization and Inhibitory Effect on Fibroblast Growth Factor−Receptor Interaction

Abstract

This study was undertaken to characterize the pectin from four citrus species and to determine their in vitro inhibitory activities on the binding of fibroblast growth factor (FGF) to the FGF receptor (FGFR). Pectin from various parts of lemon, grapefruit, tangerine, and orange were isolated and characterized. Tangerine had the highest pectin content among the four citrus species. Segment membrane contained as much as or more pectin than flavedo/albedo. Anhydrogalacturonic content was highest in pectin from segment membrane of tangerine and flavedo/albedo of grapefruit. Lemon pectin contained the highest methoxyl content (MC), and grapefruit contained the largest proportion of lower molecular weight (<10000 Da) pectin. Tangerine contained the highest neutral sugar in both flavedo/albedo and segment membrane. The interdependency of heparin on factor-receptor interaction provides a means for identifying new antagonists of growth factor activity and thus for treatment of various diseases. These results showed that pectin significantly inhibited the binding of FGF-1 to FGFR1 in the presence of 0.1 microg/mL heparin. The pectin from the segment membrane of lemon was the most potent inhibitor. The inhibition activity was significantly correlated with sugar content, MC, and size of pectin. Kinetic studies revealed a competitive nature of pectin inhibition with the heparin, a crucial component of the FGF signal transduction process. The observation that the heparin-dependent biological activity of FGF signal transduction is antagonized by citrus pectin should be further investigated for the use of these pectins as anti-growth factor agents for potential health benefits.

Bio-processing of agro-industrial waste orange peel for induced production of pectinase by Trichoderma viridi; its purification and characterization

“…70 mg/g, according to the investigations. The present reported findings are in accordance with earlier reported data from the scientists [9][10][11].…”

Isolation and Characterization of Pectin from Peel ofCitrus tankan

“…In addition to their gelation ability, the physiologically active aspects of citrus pectins have recently been studied. [11][12][13] It is possible that the pectin from C. tankan would have similar effects because of its high galacturonic acid content, low neutral sugar content, high DM and relatively high molecular mass. Modified citrus pectin (MCP) is marketed as a supplement for treating cancer and heavy metal toxicity in the United States.…”

The inhibitory effect of ginseng pectin on L-929 cell migration

“…Ginseng pectin mainly contains two types of polysaccharides, homogalacturonan and arabinogalactan with type I rhamnogalacturonan domains (Zhang et al, 2009). The homogalacturonan is rich in GalA and is structurally similar to the negatively charged polysaccharides, HA and heparin, so can compete with these endogenous polysaccharides (Liu et al, 2001) and perturb CD44/FGFR and GTPase signaling. Similarly, cell adhesion can influence migration rates (Gupton and Waterman-storer, 2006), and GP might also affect cell adhesion, although it was not tested in this study.…”