Classificação taxonômica das estirpes de rizóbio recomendadas para as culturas da soja e do feijoeiro baseada no seqüenciamento do gene 16S rRNA

Chueire, Ligia Maria Oliveira; Bangel, Eliane Villamil; Mostasso, Fábio Luís; Campo, R. J.; Pedrosa, Fábio O.; Hungría, Mariangela

doi:10.1590/s0100-06832003000500007

Cited by 30 publications

(32 citation statements)

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“…Strain BTAi 1 was included as an outgroup on the basis of its distinctive 16S RNA gene sequence (Willems et al, 2001) and was also provided by the USDA. Other B. japonicum reference strains included SEMIA 566, SEMIA 586 (=CB 1809), their natural variants SEMIA 5079 (=CPAC 15) and SEMIA 5080 (=CPAC 7), respectively, both of which have been used in Brazilian commercial inoculants for soybean since 1992 (Nishi et al, 1996;Chueire et al, 2003), and SEMIA 5085, used in soybean commercial inoculants in Argentina, all of which had their taxonomic position confirmed by the sequencing of the whole 16S rRNA gene (Chueire et al, 2003). B. elkanii reference strains included SEMIA 587 and SEMIA 5019 (=29w), both of which have also been used in Brazilian commercial inoculants for soybean since 1979 (Nishi et al, 1996;Chueire et al, 2003); their 16S rRNA genes have also been sequenced (Chueire et al, 2003).…”

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confidence: 99%

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

Germano

Menna

Mostasso

et al. 2006

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Genetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N 2 -fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76 T ; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts other than soybean. New clusters were also observed for the IGS region. The largest number of differences was detected in the analysis of the 23S rRNA gene, and 16 groups and isolated strains were joined at a very low level of similarity (16 %). In a combined analysis with the three ribosomal regions, the majority of strains isolated from soybean clustered with a similarity of 54 % with type and reference strains of B. japonicum, while most strains isolated from Brazilian indigenous legume species grouped with B. elkanii at a level of similarity of 46 %. All strains were clustered at a very low level of similarity (27 %), and at least two new clusters were clearly defined. These new clusters might be related to intraspecific differences or to novel subspecies, or even to novel species; indeed, strains from one of these clusters show higher 16S rRNA gene sequence similarity to members of the genus Burkholderia. The results obtained in this study emphasize the high level of diversity of symbiotic diazotrophic bacteria in the tropics that still remains to be determined. INTRODUCTIONThe Leguminosae (known as the Fabaceae in the USA) is one of the largest families of plants, with over 18 000 species classified into around 650 genera, representing approximately one-twelfth of all known flowering plants and occupying nearly all terrestrial biomes (Polhill & Raven, 1981). Many species within this family are capable of establishing symbioses with a group of bacteria collectively called rhizobia, of which the most important feature is the capacity...

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Section: Introductionmentioning

confidence: 99%

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

Germano

Menna

Mostasso

et al. 2006

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…In our studies, we have identified other strains, including PRF 35 T , PRF 54, CPAO 1135 and H 52, which show high efficiency in N 2 fixation with common bean, and are classified as R. tropici (Hungria et al, 2000Mostasso et al, 2002;Pinto et al, 2007). However, our research group has accumulated evidence to indicate that these strains represent a distinct species (Mostasso et al, 2002;Chueire et al, 2003;Pinto et al, 2007;Ribeiro et al, 2009). …”

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confidence: 91%

Rhizobium paranaense sp. nov., an effective N2-fixing symbiont of common bean (Phaseolus vulgaris L.) with broad geographical distribution in Brazil

Dall’Agnol

Ribeiro

Delamuta

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Nitrogen (N), the nutrient most required for plant growth, is key for good yield of agriculturally important crops. Common bean (Phaseolus vulgaris L.) can benefit from bacteria collectively called rhizobia, which are capable of fixing atmospheric nitrogen (N 2 ) in root nodules and supplying it to the plant. Common bean is amongst the most promiscuous legume hosts; several described species, in addition to putative novel ones have been reported as able to nodulate this legume, although not always effectively in terms of fixing N 2 . In this study, we present data indicating that Brazilian strains PRF 35 T , PRF 54, CPAO 1135 and H 52, currently classified as Rhizobium tropici, represent a novel species symbiont of common bean. Morphological, physiological and biochemical properties differentiate these strains from other species of the genus Rhizobium, as do BOX-PCR profiles (less than 60 % similarity), multilocus sequence analysis with recA, gyrB and rpoA (less than 96.4 % sequence similarity), DNA-DNA hybridization (less than 50 % DNA-DNA relatedness), and average nucleotide identity of whole genomes (less than 92.8.%). The novel species is effective in nodulating and fixing N 2 with P. vulgaris, Leucaena leucocephala and Leucaena esculenta. We propose the name Rhizobium paranaense sp. nov. for this novel taxon, with strain PRF 35 T (5CNPSo 120 T 5LMG 27577 T 5IPR-Pv 1249 T ) as the type strain.

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“…Os maiores avanços na utilização de técnicas de biologia molecular estão sendo obtidos pela comparação das seqüências de nucleotídeos do DNA, em especial a região que codifica o 16S rRNA, considerado conservado entre as bactérias, mas ao mesmo tempo, suficientemente variável e com uma quantidade de informações capazes de revelar, claramente, as relações filogenéticas entre as espécies (WEISBURG et al, 1991;CHUEIRE et al, 2000). Os estudos de filogenia também se beneficiaram pela técnica de PCR (Polymerase Chain Reaction), que permite a amplificação de seqüências definidas do DNA (CHUEIRE et al, 2000) (XAVIER et al, 1997).…”

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“…Os estudos de filogenia também se beneficiaram pela técnica de PCR (Polymerase Chain Reaction), que permite a amplificação de seqüências definidas do DNA (CHUEIRE et al, 2000) (XAVIER et al, 1997). Outras 10 estirpesreferência foram obtidas da Coleção de Cultura da Embrapa Agrobiologia (Seropédica, RJ).…”

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Caracterização genética de rizóbio isolados de solos no Amazonas baseada na técnica de PCR-RFLP

Chagas

Oliveira

2009

Sem. Ci. Agr.

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ResumoPoucas são as informações referentes à caracterização molecular de rizóbios nativos isolados de solos no Amazonas. Assim, o objetivo deste trabalho foi a caracterização genética de rizóbios isolados de nódulos de caupi usando o método PCR-RFLP dos genes ribossomais 16S rRNA e as enzimas de restrição HinfI, MspI, e MboI. A análise incluiu 20 isolados nativos e dez estirpes-referência de diferentes origens. Os resultados indicaram uma grande variabilidade genética com a formação de oito grupos em um dendrograma com um nível de similaridade variado. Palavras-chave: Diversidade genética, ecologia microbiana, diversidade microbiana AbstractFew are the details concerning the molecular characterization of native rhizobia isolates from Amazonas soils. The aim of the present study was the genetic characterization of rhizobia isolated from nodules of cowpea using the method PCR-RFLP of the ribosome genes 16S rRNA and the restriction enzymes HinfI, MspI, and MboI. The analysis included 20 native isolates and ten reference strains from different origins. The results indicated a great genetic variability with the formation of eight groups in one dendrogram with a varied level of similarity.

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Classificação taxonômica das estirpes de rizóbio recomendadas para as culturas da soja e do feijoeiro baseada no seqüenciamento do gene 16S rRNA

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Cited by 30 publications

References 31 publications

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

Rhizobium paranaense sp. nov., an effective N2-fixing symbiont of common bean (Phaseolus vulgaris L.) with broad geographical distribution in Brazil

Caracterização genética de rizóbio isolados de solos no Amazonas baseada na técnica de PCR-RFLP

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