2008
DOI: 10.1111/j.1365-3156.2008.02065.x
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Clonal heterogeneity of clinical isolates of vancomycin‐resistant Enterococcus faecium with unique vanS

Abstract: Summaryobjective To examine the clonal diversity of vancomycin-resistant enterococci (VRE). methods A total of 900 clinical isolates of enterococci were obtained, and VRE isolates were subjected to antimicrobial susceptibility tests, biochemical fingerprinting with the PhPlate system (PhP), ribotyping and pulsed-field gel electrophoresis (PFGE) typing.results Forty-nine of all enterococcal isolates were resistant to high levels of vancomycin (MIC ‡ 128) and identified as Enterococcus faecium. Biochemical finge… Show more

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Cited by 15 publications
(6 citation statements)
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“…These phenotypes can be conjugally transferred to micro-organisms in human gastrointestinal tract (Launay et al 2006). Heterogeneity has been reported in nonessential regions of Tn1546 (Handwerger et al 1995;Park et al 2007;Pourshafie et al 2008). In this study, we showed that frog VRE isolates harbour intact Tn1546 elements and it is identical to Tn1546 elements of human VRE isolates, suggestive of their close genetic relatedness.…”
Section: Discussionsupporting
confidence: 56%
See 1 more Smart Citation
“…These phenotypes can be conjugally transferred to micro-organisms in human gastrointestinal tract (Launay et al 2006). Heterogeneity has been reported in nonessential regions of Tn1546 (Handwerger et al 1995;Park et al 2007;Pourshafie et al 2008). In this study, we showed that frog VRE isolates harbour intact Tn1546 elements and it is identical to Tn1546 elements of human VRE isolates, suggestive of their close genetic relatedness.…”
Section: Discussionsupporting
confidence: 56%
“…2000). A cluster of nine genes (ORF1 and ORF2), regulation (VanR and VanS), resistance to glycopeptides (VanH, VanA and VanX) and synthesis of peptidoglycan (VanY and VanZ) mediating high level of resistance to both Vn and Tp have been reported (Pourshafie et al. 2008).…”
Section: Discussionmentioning
confidence: 99%
“…In our study, VREfm were classified into 38 types (23 clonal types and 15 singletons), demonstrating a high genetic heterogeneity. A similar polyclonal distribution of VREfm has also been observed in other studies (Landerslev et al, 2016;Pourshafie et al, 2008;Somily et al, 2016;J. Top et al, 2008).…”
Section: Discussionsupporting
confidence: 88%
“…Similarity among the isolates was calculated as the correlation coefficient after a pair-wise comparison of the biochemical fingerprints and clustered according to the unweighted pair group method (UPGMA) with arithmetic averages. 14 For PFGE, a single and well-isolated colony grown on blood agar was inoculated into 10 ml of tryptic soy broth (TSB) and grown on a reciprocal shaker at 37 8C overnight. A bacterial suspension with 150 ml EC buffer (100 mM EDTA, 1 M NaCl, 6 mM Tris-Hcl, 0.2% deoxycholate, 0.5% sodium lauroyl sarcosine, 0.5% Brij-58) containing 20 ml lysostaphin (20 mg/ml) was added to 150 ml molten agarose gel and placed in plug molds.…”
Section: Typing Of Mrsa Isolatesmentioning
confidence: 99%