Co-expression of S100A14 and S100A16 correlates with a poor prognosis in human breast cancer and promotes cancer cell invasion

Tanaka, Mizuko; Ichikawa‐Tomikawa, Naoki; Shishito, Namiko; Nishiura, Keisuke; Miura, Tomiko; Hozumi, Ayumi; Chiba, Hideki; Yoshida, Sayaka; Ohtake, Tohru; Sugino, Takashi

doi:10.1186/s12885-015-1059-6

Cited by 62 publications

(62 citation statements)

References 27 publications

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“…This member of the S100 protein family has been reported to be involved and overexpressed in several pathological affections, including cancer, but with different and often contradictory roles [119][120][121]. In our tumor tissues we found a single form of S10AG with a 3x fold increase versus the normal tissues.…”

Section: S10ag (S100a16)mentioning

confidence: 49%

Retrospective Proteomic Screening of 100 Breast Cancer Tissues

Pucci‐Minafra¹,

Cara²,

Musso³

et al. 2017

Preprint

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Abstract:The present investigation has been conducted on one hundred tissue fragments of breast cancer, collected and immediately cryopreserved following the surgical resection. The fragments were selected from patients with invasive ductal carcinoma of the breast, the most common and potentially aggressive type of mammary cancer, with the objective to increase the knowledge of breast cancer molecular markers, useful for diagnostic and prognostic categorization of patients, in assessing postsurgical therapeutic regimes. The proteomic screening, by 2D-IPG and mass spectrometry, allowed us to identify two main classes of protein clusters: proteins expressed ubiquitously at high levels in all patients, and proteins expressed sporadically among the same patients. Within the group of ubiquitous proteins, glycolytic enzymes and proteins with anti-apoptotic activity were predominant. Among the sporadic ones, proteins involved in cell motility, molecular chaperones and proteins involved in the detoxification appeared prevalent. The data of the present study indicates that the primary tumor growth is generally supported by two concurrent pathways: the inhibition of apoptosis and the stimulation of cellular proliferation. The second phase of the evolution of the tumor can be prematurely scheduled by the occasional presence of proteins involved in cell motility and in the defenses of the oxidative stress. To our knowledge this report on large-scales proteomics of breast cancer is currently a unique approach in the literature that offers the opportunity to evaluate the presence and recurrence of proteins to be used as prognostic indicators and susceptibility to metastasis in patients operated on for invasive ductal carcinoma of the breast.

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Section: S10ag (S100a16)mentioning

confidence: 49%

Retrospective Proteomic Screening of 100 Breast Cancer Tissues

Pucci‐Minafra¹,

Cara²,

Musso³

et al. 2017

Preprint

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“…There are no reports on the expression of S100A16 in cervical carcinoma. It has been reported that a high S100A16 expression level is correlated to poor prognosis in breast cancer and lung adenocarcinoma (27,28), whereas a low S100A16 expression level is correlated with reduced survival and poor tumor differentiation in oral squamous cell carcinoma (29). Further studies are needed to clarify the function of S100A16 and its clinical significance in the various cancer types.…”

Section: Discussionmentioning

confidence: 99%

S100A16 up‑regulates Oct4 and Nanog expression in cancer stem‑like cells of Yumoto human cervical carcinoma cells

et al. 2018

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Abstract. Cancer stem-like cells (CSCs), which possess the ability to self-renewal and are multipotent, are regarded as the cause of tumor formation, recurrence, metastasis and drug resistance. It is necessary to understand the properties of CSCs in order to treat them effectively. It has been previously reported that S100 family proteins, which carry calcium-binding EF-hand motifs and are associated with tumorigenic processes, serve crucial roles in maintaining cancer stem-like properties. S100A16 is upregulated in various types of cancer, including bladder, lung and pancreatic. However, the roles of S100A16 in cancer cells, particularly CSCs, are not clear. The present study investigated the roles of S100A16 in CSCs using the sphere formation assay of Yumoto cells, which are a human cervical carcinoma cell line. The mRNA expression levels were evaluated by reverse transcription-polymerase chain reaction and the protein expression levels were detected by western blot analysis. Following the sphere formation of Yumoto cells, the mRNA and protein expression level of Oct4, Nanog and S100A16 were increased compared with the control cells. Following transfection with S100A16 small interfering RNA (siRNA), the mRNA and protein expression of Oct4 and Nanog were decreased and the spheroid size was significantly decreased in the sphere formation of Yumoto cells compared with control siRNA treated cells. There was no change in the p53 mRNA expression level, whereas the p53 protein expression level, which was decreased by the sphere formation, was recovered by S100A16 knockdown. In addition, the protein expression levels of Oct4 and Nanog, which were increased in the sphere formation, were decreased by the proteasome inhibitor lactacystin. No differences were observed in the S100A16 protein expression between the presence or absence of lactacystin. These results suggest that S100A16 serves an important role in the CSCs of human cervical carcinoma and is a positive regulator of Oct4 and Nanog.

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“…In most cases, absent or faint cytoplasmic staining and no nuclear staining were observed. S100A14 expression was found to be mainly localized in the cytoplasm in ovarian cancer [13], in the cytoplasm and nucleus in hepatocellular carcinoma [14], and on the cell membrane in breast cancer [23]. These different staining patterns suggest that S100A14 localization and function are different in organ-specific cancers.…”

Section: Discussionmentioning

confidence: 97%

“…The breast cancer staining pattern (mainly cell membrane) is similar to lung adenocarcinoma. Tanaka et al [23] reported that in the breast cancer cell lines, S100A14 expression on the membrane along the lateral cell surface was due to interaction and binding with actin; therefore, S100A14 may promote cancer cell migration and invasion. However, interaction between S100A14 and actin in lung adenocarcinoma is unknown, and further analysis is needed.…”

Section: Discussionmentioning

confidence: 99%

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Clinicopathological Significance of S100A14 Expression in Lung Adenocarcinoma

Katono

Sato

Kobayashi³

et al. 2017

Oncol Res Treat

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Background: Several studies have reported that S100A14 plays important roles during different steps of the tumorigenic process and tumor progression of several types of cancer. The aim of the present study was to investigate the clinicopathological and prognostic significance and functional roles of S100A14 in lung adenocarcinoma. Patients and Methods: S100A14 expression was immunohistochemically studied in 166 consecutive resected lung adenocarcinomas, and its correlation with clinicopathological parameters was evaluated. Functional roles of S100A14 in lung adenocarcinoma were investigated based on invasion and migration assays on a small interfering (si)RNA-treated lung adenocarcinoma cell line. Results: S100A14 expression was detected in 82 of the 166 (49.4%) lung adenocarcinomas. S100A14 expression was significantly correlated with sex, poorer tumor differentiation, higher disease stages, larger tumor size, lymph node metastasis, intratumoral vascular invasion, intratumoral lymphatic invasion, pleural invasion, and poorer prognosis. Invasion and wound healing assays showed that S100A14 siRNA knockdown cells had significantly decreased invasion and migration abilities compared with siRNA control cells. Conclusion: S100A14 is expressed in a subset of lung adenocarcinoma, and its expression is related to certain clinicopathological parameters. Furthermore, S100A14 expression was strongly correlated with migration and invasion in lung adenocarcinoma cells.

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Co-expression of S100A14 and S100A16 correlates with a poor prognosis in human breast cancer and promotes cancer cell invasion

Cited by 62 publications

References 27 publications

Retrospective Proteomic Screening of 100 Breast Cancer Tissues

Retrospective Proteomic Screening of 100 Breast Cancer Tissues

S100A16 up‑regulates Oct4 and Nanog expression in cancer stem‑like cells of Yumoto human cervical carcinoma cells

Clinicopathological Significance of S100A14 Expression in Lung Adenocarcinoma

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