Coexistence and upregulation of three types of opioid receptors, mu, delta and kappa, in human hypertrophic scars

Cheng, Biao; Hw, Liu; Xb, Fu; Zy, Sheng; Jf, Li

doi:10.1111/j.1365-2133.2008.08449.x

Cited by 71 publications

(57 citation statements)

References 28 publications

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“…However, excessive collagen deposition in the incisional wound results in hypertrophic scars and keloids [34]. Furthermore, increased expression of opioid receptors was also detected in the human hypertrophic scar [35]. Therefore, modulation of the wound healing process by exogenous or endogenous opioids involves multifactorial physiological responses, and the biological effects of morphine on the healing of incisional wound require further clinical investigation.…”

Section: Discussionmentioning

confidence: 98%

Morphine enhances tissue content of collagen and increases wound tensile strength

Chang

Chen²,

Huang

et al. 2010

J Anesth

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Purpose Morphine is a commonly prescribed analgesic for wound pain. Previous studies have shown that morphine enhances accumulation of collagen in cultured fibroblasts. Because fibroblasts are important for the remodeling of connective tissue in incisional wound, this study investigates the biological effects of morphine on cutaneous collagen content and wound tensile strength. Methods A full-thickness incisional wound (2 cm in length) was created on the dorsum of mice followed by treatment with placebo or morphine (5 and 20 mg/kg/day, i.p.). Fourteen days later, tensile strength of the healed incisional wound was measured using a tensiometer. Protein expression of transforming growth factor (TGF)-b1 and matrix metalloproteinases (MMP)-2 in the incisional wound tissue was analyzed. Degree of tissue remodeling and levels of collagen were determined by histological examination and a dye-binding collagen assay, respectively. Results Morphine enhanced the breaking strength of incisional wound 14 days after treatment (92 ± 10, 102 ± 10 and 134 ± 12 mg for control, morphine 5 mg/ kg/day and morphine 20 mg/kg/day, respectively; P = 0.03, n = 6-7). Protein expression of TGF-b1 and MMP-2 was significantly enhanced in mice treated with morphine.

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Section: Discussionmentioning

confidence: 98%

Morphine enhances tissue content of collagen and increases wound tensile strength

Chang

Chen²,

Huang

et al. 2010

J Anesth

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“…GFP transgenic fibroblasts were isolated from GFP transgenic neonatal mice as previously described [17]. Briefly, the obtained skin tissue was washed with PBS three times and cut into approximately 0.5 cm 3 pieces.…”

Section: Cell Adhesion Testmentioning

confidence: 99%

Novel bilayer wound dressing composed of silicone rubber with particular micropores enhanced wound re-epithelialization and contraction

et al. 2015

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“…The fibroblasts were isolated from the neonatal mice according to the previous study. 32 Briefly, the skin tissue of neonatal mice was immersed in 0.5 mg/mL Dispase II (Sigma-Aldrich Co.) at 4°C overnight to separate the epidermis and dermis. Then, the isolated dermis was minced and digested by trypsin (0.25 mg/mL; Boster, Wuhan, People's Republic of China) for 10 min.…”

Section: Bacterial Suspension Assay and Determination Of Biofilm Formmentioning

confidence: 99%

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Liu¹,

Luo²,

Wang³

et al. 2017

IJN

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Bacterial infection is a major hurdle to wound healing, and the overuse of antibiotics have led to global issue, such as emergence of multidrug-resistant bacteria, even “super bacteria”. On the contrary, nanosilver (NS) can kill bacteria without causing resistant bacterial strains. In this study, NS was simply generated in situ on the polycaprolactone (PCL) nanofibrous mesh using an environmentally benign and mussel-inspired dopamine (DA). Scanning electron microscopy showed that NS uniformly formed on the nanofibers of PCL mesh. Fourier transform infrared spectroscopy revealed the step-by-step preparation of pristine PCL mesh, including DA coating and NS formation, which were further verified by water contact angle changing from hydrophobic to hydrophilic. To optimize the NS dose, the antibacterial activity of PCL/NS against Staphylococcus aureus , Escherichia coli and Acinetobacter baumannii was detected by bacterial suspension assay, and the cytotoxicity of NS was evaluated using cellular morphology observation and Cell Counting Kit-8 (CCK8) assay. Then, inductively coupled plasma atomic emission spectrometry exhibited that the optimized PCL/NS had a safe and sustained silver release. Moreover, PCL/NS could effectively inhibit bacterial infection in an infectious murine full-thickness skin wound model. As demonstrated by the enhanced level of proliferating cell nuclear antigen (PCNA) in keratinocytes and longer length of neo-formed epidermis, PCL/NS accelerated wound healing by promoting re-epithelialization via enhancing keratinocyte proliferation in infectious wounds.

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Coexistence and upregulation of three types of opioid receptors, mu, delta and kappa, in human hypertrophic scars

Abstract: Our results demonstrate that expression of three types of ORs, MOR, DOR and KOR, was markedly upregulated in human hypertrophic scars, suggesting a possible link between upregulated ORs and local cacaesthesia in hypertrophic scars.

Cited by 71 publications

References 28 publications

Morphine enhances tissue content of collagen and increases wound tensile strength

Morphine enhances tissue content of collagen and increases wound tensile strength

Novel bilayer wound dressing composed of silicone rubber with particular micropores enhanced wound re-epithelialization and contraction

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

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