COLLAGEN EXTRACTION FROM YELLOWFIN TUNA (Thunnus albacares) SKIN AND ITS ANTIOXIDANT ACTIVITY

Abstract

Tuna skin, a byproduct of the fish processing industry, is used as an alternative collagen source to replace bovine and porcine products. This study aimed to extract collagen from tuna skin with acetic acid, and investigated the antioxidant activity. Collagen extraction was carried out through a pretreatment process, defatted with butyl alcohol, and soaking in acetic acid to extract the Acid Soluble Collagen (ASC). The effect of concentration of sodium hydroxide and soaking time on the non-collagenous protein removed were measured, and evaluated. The yield and antioxidant activity of each sample were evaluated and the best result was determined by ANOVA. The highest yield of collagen was 3.18% based on dry weight reached at the treatment with sodium hydroxide 0.2 M and acetic acid 1 M. The different treatments did not result in any significant differences in the spectrum of amide A, B, I, II and III which are the characteristics spectra of collagen. Based on the electrophoretic pattern, tuna skin collagen has two  chains (1 and 2), and one β chain. Therefore, it is classified as type I collagen. The main amino acids were glycine and proline. In addition, the strongest antioxidant activity was found in the sample treated with sodium hydroxide 0.05 M and acetic acid 1 M treatment with IC50 value of 0.45 mg/mL. This study is the first to report on antioxidant activity from fish collagen (not hydrolysate or peptide products).

Characterization and Antioxidant Activity of Collagen, Gelatin, and the Derived Peptides from Yellowfin Tuna (Thunnus albacares) Skin

“…Tuna bones and skins are generally used only to feed cattle with reduced selling price. For value addition, the skin waste from industrial tuna loins as well as fillets can be utilized as the materials for collagen and gelatin [5][6][7]. However, a large amount of tuna skin has remained underutilized, despite the fact that it can be an excellent source of collagen and gelatin, provided that they are demonstrated to be of high quality.…”

Marine collagen: purification, properties and application

“…Skin, muscle tissue (Yoo et al, 2008;Nurilmala et al, 2019b) Priacanthus tayenus Tissues, skin (Jongjareonrak et al, 2005;Jongjareonrak, 2006;La Noce et al, 2014;…”

Peningkatan Nilai Tambah Kulit Ikan Tuna sebagai Bahan Baku Pupuk Organik Cair

“…YPT yang berada di Kota Banda Aceh menghasilkan limbah kulit ikan tuna sirip kuning yang melimpah dan belum dimanfaatkan secara optimal. Kulit ikan tuna mengandung protein yang tinggi sehingga beberapa peneliti tertarik untuk memanfaatkan limbah tersebut menjadi biomaterial yang bernilai ekonomi tinggi seperti gelatin (Firdausiah et al, 2021;Montero & Acosta, 2020;Moranda et al, 2018;Ningrum et al, 2020;Nurilmala et al, 2017;Nurwulandari et al, 2020;Sousa et al, 2017), kolagen (Jafari et al, 2020;Nurilmala et al, 2019;Nurjannah et al, 2021), dan bahan emulsi (Kumar et al, 2019). Namun demikian pemanfaatan kulit ikan tuna belum secara spesifik mengarah pada produk pupuk, khususnya POC.…”