Comparação das técnicas de captura de híbridos e PCR para a detecção de HPV em amostras clínicas

Rodrigues, Adriana Dalpicolli; Cantarelli, Vlademir Vicente; Frantz, Miriam Alice; Pilger, Diogo André; Pereira, Fabiana de Souza

doi:10.1590/s1676-24442009000600004

Cited by 13 publications

(21 citation statements)

References 28 publications

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“…Of the 31 viral types identified in our study, 17 (34,53,54,55,61,candHPV62,66,67,70,71,73,81,83,84,candHPV85,candHPV89,HPV114) representing 19.3% of all samples and 57.0% of the HPV DNA positive samples and which include 7 high-risk types (34,53,66,67,70,73,candHPV85) are not within the detection spectrum of the Hybrid Capture ® test. Furthermore, a recent study comparing PCR and hybrid capture methods demonstrated the higher sensitivity of PCR to detect HPV DNA in clinical samples (Rodrigues et al, 2009). Taken together, these findings justify the use of PCR as a tool for gathering information on HPV infection.…”

Section: Discussionmentioning

confidence: 82%

Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil

Coser¹,

T²,

Simon³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Human papillomavirus (HPV) infection is a common viral sexually transmitted infection and the main cause of cervical cancer in women worldwide. Epidemiological data on the prevalence of HPV in a given population is essential for the establishment of effective prevention strategies. The aim of this study was to determine HPV prevalence in women who attended a public health service within an urban center in Brazil. Cervical samples were collected from 337 women recruited from a primary public health care clinic in the city of Cruz Alta located in Rio Grande do Sul, the were detected in 83 (72.8%) and 48 (42.1%) samples, respectively. Furthermore, a great diversity of HPV genotypes was observed (18 high-risk, 12 low-risk, and 1 indeterminate). The most commonly identified low-risk types were candHPV62 (7.9%) and 61 (5.3%), while the most common high-risk types were 16 and 33 (8.8% each). Abnormal cytology was observed in 10 (3.0%) women, 9 of which were infected with HPV. Of the remaining 327 women with normal cytology results, 107 (32.7%) were positive for HPV DNA. HPV infection was correlated with younger age (less than 40 years), a first Pap smear, and other vaginal infections.

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Section: Discussionmentioning

confidence: 82%

Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil

Coser¹,

T²,

Simon³

et al. 2013

Genet. Mol. Res.

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“…Molecular diagnosis of HPV infection is important for virus screening, and is mainly based on methods such as: hybrid capture (CH2) (20,23) , in situ hybridization (2) , and PCR (8,21,24) . These techniques have vary widely in terms of sensitivity and specificity, and PCR is the most used today in various areas of molecular diagnostics due to its great ability to detect small fragments of deoxyribonucleic acid (DNA) (4,11) .…”

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confidence: 99%

HPV detection using primers MY09/MY11 and GP5+/GP6+ in patients with cytologic and/or colposcopic changes

Venceslau¹,

Bezerra²,

Lopes³

et al. 2014

Jornal Brasileiro De Patologia E Medicina Laboratorial

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Introduction: Cervical cancer is one of the most common diseases among women, and cause considerable morbidity and mortality. Considering that cervical cancer is an important neoplasia in northeastern Brazil, and the prevalence of high-risk human papillomavirus (HPV) is directly associated with it, this work had aimed to correlate the cytological and/or colposcopic findings with HPV infection status, and verify the performance of MY09/MY11 and GP5+/6+ primers for HPV detection. Material and method: Patients in this study were from Penedo-AL, a city with high level of poverty (poverty rate of 60.62%). Out of 70 patients with cytological and/or colposcopic changes, 32 agreed to participate in the study. Results: Regarding cytology, 21 (30%) patients presented atypical squamous cells of undetermined significance (ASC-US); 20 (29%), atypical glandular cells of undetermined significance (AGUS); 12 (17%), low-grade intraepithelial lesion (LSIL); five (7%), high-grade intraepithelial lesion (HSIL); and 12 (17%), positive colposcopy. From these, 27 (84%) presented the band gene encoding for human ß-globin. From the 27 patients, eight (30%) were positive for HPV. The results showed that the deoxyribonucleic acid (DNA) of HPV was detected in 15% and 30% by using MY-PCR and GP +-PCR, respectively. Conclusion: This study suggests that more than one type of oligonucleotide primer should be used in clinical samples to increase sensitivity for the detection of HPV.

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“…Thus, screening for cervical cancer through molecular biology tests began to be considered a strategy for early screening of the virus in women. Among the methods currently available for HPV detection are hybrid capture (HCII), polymerase chain reaction (PCR), solid-phase hybridization (microrrays), and in situ hybridization (14) . Although all methods can be used for this purpose, only the HCII test, a qualitative test, is approved by the Food and Drug Administration and the Brazilian Health Surveillance Agency for the diagnosis of HPV (15) .…”

Section: Introductionmentioning

confidence: 99%

“…These PCR is based on the specific amplification of segments of the HPV genome, and has the potential to detect very low levels of viral load in cells and tissues, even in latent infections (14) .…”

Section: Introductionmentioning

confidence: 99%

Frequency and genotyping of human papillomavirus in women submitted to citology

Silva¹,

Vietta²,

Golfetto³

et al. 2015

J Bras Doenças Sex Transm

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Introduction: Among the sexually transmitted virus, the human papilloma virus (HPV) is the most prevalent and may be detected a considerable number of sexually active women. He is considered the main agent of cervical cancer. Therefore, the high-risk HPV identification can aid in the prevention of cervical lesions. Objective: To evaluate the occurrence of HPV infections, comparing different methodologies, as well as some risk factors and their potential association in the development of cervical cancer in women submitted to cytopathology treated in ambulatory Unit Family and Community Health (USFC) of the University of Vale do Itajaí (UNIVALI). Methods: 118 samples were evaluated sexually active women who sought care for screening of cervical cancer in USFC and UNIVALI. All samples were subjected to polymerase chain reaction (PCR) and the liquid and conventional cytology. However, only 64 women were subjected to hybrid capture methodology (CH2). Results: The prevalence of HPV was 43.22% by PCR and 25% for CH2; analysis of the results was observed association between HPV and the following variables: ethnicity (p<0.016), scholarity (p<0.012), human immunodeficiency virus (HIV) (p<0.008), preservative (p<0.02), oral contraceptives (p<0.03), younger age at first sexual intercourse (p<0.07), conventional cytology (p<0.002) and liquid cytology (p<0.029). Conclusion: The incidence of HPV infection is high and the high-risk HPV was primarily associated with the younger age at first sexual intercourse. Keywords: papillomaviridae; polymerase chain reaction; sexually transmitted diseases. RESUMOIntrodução: Dentre os vírus de transmissão sexual, o papilomavírus humano (HPV) é o mais prevalente, podendo ser detectado em considerável número de mulheres sexualmente ativas. Ele é considerado o principal agente causador do câncer do colo do útero. Portanto, a identificação do HPV de alto risco pode auxiliar na prevenção de lesões do colo uterino. Objetivo: Avaliar a ocorrência de infecções pelo HPV, comparando diferentes metodologias, assim como alguns fatores de risco e seu potencial de associação no desenvolvimento do câncer do colo uterino em mulheres submetidas à citopatologia atendidas nos ambulatórios da Unidade de Saúde Familiar e Comunitária (USFC) da Universidade do Vale do Itajaí (UNIVALI). Métodos: Foram avaliadas 118 amostras de mulheres sexualmente ativas que buscaram atendimento para rastreio do câncer cervical na USFC e da UNIVALI. Todas as amostras foram submetidas à reação em cadeia da polimerase (PCR) e às citologias líquida e convencional. Entretanto, apenas 64 mulheres foram submetidas à metodologia de captura híbrida (CH2). Resultados: A prevalência do HPV foi de 43,22% pela técnica de PCR e de 25% pela CH2; na análise dos resultados observou-se associação do HPV com as seguintes variáveis: etnia (p<0,016), escolaridade (p<0,012), vírus da imunodeficiência humana (HIV) (p<0,008), preservativo (p<0,02), anticoncepcional (p<0,03), início da atividade sexual (p<0,07), citologia convencional (p<0,002) e...

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Comparação das técnicas de captura de híbridos e PCR para a detecção de HPV em amostras clínicas

Cited by 13 publications

References 28 publications

Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil

Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil

HPV detection using primers MY09/MY11 and GP5+/GP6+ in patients with cytologic and/or colposcopic changes

Frequency and genotyping of human papillomavirus in women submitted to citology

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