Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis

Sultankulova, Kulyaisan; Chervyakova, O. V.; Kozhabergenov, Nurlan; Shorayeva, Kamshat; Strochkov, Vitaliy; Orynbayev, Mukhit; Sandybayev, Nurlan; Sansyzbay, Abylay; Васин, А. В.

doi:10.1155/2014/620580

Cited by 6 publications

(5 citation statements)

References 26 publications

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“…There is limited information on the diagnostic utility of these new tests. A study by Sultankulova et al compared viral isolation of Influenza A with DNA microarray technology, reverse transcription PCR and real-time PCR [ 23 ]. Microarray technology showed a higher sensitivity (99.5%) and similar specificity (98.5%) to real-time PCR.…”

Section: Discussionmentioning

confidence: 99%

Viral Infection in Adults with Severe Acute Respiratory Infection in Colombia

Remolina

Ulloa

Vargas³

et al. 2015

PLoS ONE

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ObjectivesTo identify the viral aetiology in adult patients with severe acute respiratory infection (SARI) admitted to sentinel surveillance institutions in Bogotá in 2012.DesignA cross-sectional study was conducted in which microarray molecular techniques for viral identification were used on nasopharyngeal samples of adult patients submitted to the surveillance system, and further descriptions of clinical features and relevant clinical outcomes, such as mortality, need for critical care, use of mechanical ventilation and hospital stay, were obtained.SettingRespiratory infections requiring hospital admission in surveillance centres in Bogotá, Colombia.ParticipantsNinety-one adult patients with acute respiratory infection (55% were female).MeasurementsViral identification, intensive care unit admission, hospital stay, and mortality.ResultsViral identification was achieved for 63 patients (69.2%). Comorbidity was frequently identified and mainly involved chronic pulmonary disease or pregnancy. Influenza, Bocavirus and Adenovirus were identified in 30.8%, 28.6% and 18.7% of the cases, respectively. Admission to the intensive care unit occurred in 42.9% of the cases, while mechanical ventilation was required for 36.3%. The average hospital stay was 9.9 days, and mortality was 15.4%. Antibiotics were empirically used in 90.1% of patients.ConclusionsThe prevalence of viral aetiology of SARI in this study was high, with adverse clinical outcomes, intensive care requirements and high mortality.

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Section: Discussionmentioning

confidence: 99%

Viral Infection in Adults with Severe Acute Respiratory Infection in Colombia

Remolina

Ulloa

Vargas³

et al. 2015

PLoS ONE

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“…The diagnosis of virus types based on microarray technology can improve the quality and shorten the analysis duration in molecular diagnosis of infectious diseases. Moreover, it can satisfy the needs of simultaneous detection of multiple viruses and screening large numbers of pathology samples [32, 33]. Microarray assays for avian diseases have been previously reported.…”

Section: Discussionmentioning

confidence: 99%

Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases

Xiao

Yan

et al. 2019

BMC Vet Res

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Background Avian influenza virus (AIV), infectious bronchitis virus (IBV), and Newcastle disease virus (NDV) are important avian pathogens that can cause enormous economic loss on the poultry industry. Different respiratory etiological agents may induce similar clinical signs that make differential diagnosis difficult. Importantly, AIV brings about severe threat to human public health. Therefore, a novel method that can distinguish these viruses quickly and simultaneously is urgently needed. Results In this study, an oligonucleotide microarray system was developed. AIV, including H5, H7, and H9 subtypes; NDV; and IBV were simultaneously detected and differentiated on a microarray. Three probes specific for AIV, NDV, and IBV, as well as three other probes for differentiating H5, H7, and H9 of AIV, were first designed and jet-printed to predetermined locations of initiator-integrated poly(dimethylsiloxane) for the synchronous detection of the six pathogens. The marked multiplex reverse transcription polymerase chain reaction (PCR) products were hybridized with the specific probes, and the results of hybridization were read directly with the naked eyes. No cross-reaction was observed with 10 other subtypes of AIV and infectious bursal disease virus, indicating that the oligonucleotide microarray assay was highly specific. The sensitivity of the method was at least 100 times higher than that of the conventional PCR, and the detection limit of NDV, AIV, H5, H7, and H9 can reach 0.1 EID 50 (50% egg infective dose), except that of IBV, which was 1 EID 50 per reaction. In the validation of 93 field samples, AIV, IBV, and NDV were detected in 53 (56.99%) samples by oligonucleotide microarray and virus isolation and in 50 (53.76%) samples by conventional PCR. Conclusions We have successfully developed an approach to differentiate AIV, NDV, IBV, H5, H7, and H9 subtypes of AIV using oligonucleotide microarray. The microarray is an accurate, high-throughput, and relatively simple method for the rapid detection of avian respiratory viral diseases. It can be used for the epidemiological surveillance and diagnosis of AIV, IBV, and NDV. Electronic supplementary material The online version of this article (10.1186/s12917-019-1985-7) contains supplementary material, which is available to authorized users.

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“…El porcentaje de muestras positivas para virus influenza en nuestra cohorte es similar a países de otros continentes durante el mismo periodo de observación 12,13 . Nuestros resultados se relacionan con lo documentado por Arango AE y colaboradores, en el Hospital Pablo Tobón Uribe en la ciudad de Medellín durante los años 2007 a 2012, donde detectaron virus influenza en 20,8% de los casos, aunque con la ventaja de emplear RT-PCR y cultivo viral 14 y con dos estudios descriptivos con los mismos 5 años de observación (2009 a 2013), realizados en India 15 y Niger 16 , donde influenza A(H1N1)pdm09 es el más frecuente de todos los virus influenza, seguido de A(H3N2) e influenza B. Nuestra positividad es baja comparada con lo descrito por Remolina YA y colaboradores, en casos de IRAG atendidos en 7 hospitales de tercer nivel en la ciudad de Bogotá durante el año 2012, donde documentaron infección viral en 69,2%, y 30,8% de influenza, de los cuales influenza A correspondía a 75% 17 , aunque el diagnóstico molecular para este estudio se hizo mediante la técnica de micro matrices para detección de genoma viral, que puede llegar a tener una mayor sensibilidad que la RT-PCR 18 .…”

Section: Discussionunclassified

Descripción de características demográficas y clínicas de una cohorte pacientes fallecidos por infección respiratoria aguda en Colombia durante los años 2009 a 2013

Santiago

Barbosa²,

Trujillo

2019

Infect

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Objetivo: Describir las características clínicas, demográficas, aislamientos virales y hallazgos de histopatología de individuos que fallecieron por Infección Respiratoria Aguda (IRA) y que fueron notificados al Instituto Nacional de Salud (INS) entre los años 2009 y 2013.Métodos: Estudio observacional, descriptivo, retrospectivo, basado en la revisión de fichas epidemiológicas y reportes de estudios de virología e histopatología de muestras respiratorias de individuos fallecidos con diagnóstico de IRAResultados: De 1604 personas fallecidas se encontró que, 55% fueron hombres, 46,5% de los individuos tenía entre 20 y 59 años. La RT-PCR fue positiva en 18,3% de los casos, los virus más frecuentes fueron: influenza A(H1N1)pdm09 13,9%, A(H3N3) 1,9% e influenza B 0,5%. La letalidad de IRA fue mayor en los individuos que recibieron antiviral o antibiótico OR 2,80 (IC 95% 2,29 - 3,43) y 3,19 (IC 95% 2,63 – 3,86), respectivamente.Conclusión: El virus influenza A(H1N1) pdm09 fue el principal agente identificado en los casos fatales de IRA confirmada por laboratorio durante los años 2009 a 2013, con mayor letalidad en individuos entre 20 y 59 años; 64,7% de los casos fatales presentaron neumonitis. Se debe aclarar si el inicio de antivirales afecta el pronóstico en los casos graves de IRA.

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Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis

Cited by 6 publications

References 26 publications

Viral Infection in Adults with Severe Acute Respiratory Infection in Colombia

Viral Infection in Adults with Severe Acute Respiratory Infection in Colombia

Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases

Descripción de características demográficas y clínicas de una cohorte pacientes fallecidos por infección respiratoria aguda en Colombia durante los años 2009 a 2013

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