Comparative in vitro Antidiabetic and Antioxidant Activity of Various Extracts of Ficus Species

Abusufyan, Shaikh; Ibrahim, Mohammed; Khan, Mateen A.

doi:10.5530/pj.2018.2.59

Cited by 11 publications

(5 citation statements)

References 7 publications

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“…This result is in line with some previous reports with other plants. 2,49 –52 The aqueous-methanol fraction may contain compounds that are structurally analogous to the natural substrates of the enzymes like phenolics, flavonoid glycosides, and saponins and could interfere with the normal activity of the enzymes. The extracts showed a relatively higher inhibition of glucosidase, an indication that they may have a relative advantage of lesser or no side effects compared to the standard drug.…”

Section: Discussionmentioning

confidence: 99%

Methanol-Extract/Fractions of Dacryodes edulis Leaves Ameliorate Hyperglycemia and Associated Oxidative Stress in Streptozotocin-Induced Diabetic Wistar Rats

Ononamadu

Alhassan

Ibrahim

et al. 2019

J Evid Based Complementary Altern Med

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The present study evaluated the antidiabetic and antioxidant potential of the methanolic extract/solvent fractions of the leaves of Dacryodes edulis using a streptozotocin (STZ)-induced diabetic Albino Wistar rat model. The fasting blood glucose/insulin levels and inhibition of α-amylase and α-glucosidase were determined. Antioxidant activity was assessed in vitro by 2,2-diphenyl-1-picrylhydrazyl, hydroxyl, superoxide scavenging, reducing power, and total antioxidant capacity assays and in vivo by monitoring catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and reduced glutathione (GSH) and malondialdehyde (MDA) levels. The aqueous-methanol fraction exhibited the highest and significant ( P < .05) reduction in fasting blood glucose (FBG; 54.03%) with a concomitant inhibition of α-amylase and α-glucosidase activities. The ethyl acetate fraction also exhibited a significant ( P < .05) reduction in FBG and an increase in insulin levels in the treated diabetic Wistar rats. A significantly ( P < .05) higher reducing power and radical scavenging activity was observed in the aqueous-methanol and ethyl acetate fractions. The aqueous-methanol and ethyl acetate fractions also significantly ( P < .05) reversed the alterations in oxidative stress markers (GSH, MDA, CAT, and SOD) observed in the diabetic control group. In conclusion, the study demonstrated that the methanol extract of Dacryodes edulis ameliorates hyperglycemia and the associated oxidative stress in STZ-induced diabetic Wistar rats. These observed activities are largely due to the compounds that partitions into the aqueous-methanol (55:45) solvent fraction. This provides scientific evidence for the use of this plant extract in folk medicine and also a baseline data for its further characterization. Further work should be carried out to characterize the aqueous-methanol solvent fractions for the active compounds.

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Section: Discussionmentioning

confidence: 99%

Methanol-Extract/Fractions of Dacryodes edulis Leaves Ameliorate Hyperglycemia and Associated Oxidative Stress in Streptozotocin-Induced Diabetic Wistar Rats

Ononamadu

Alhassan

Ibrahim

et al. 2019

J Evid Based Complementary Altern Med

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“…Isophthalic acid Antidiabetic (Patel et al, 2016); (Acta, 2021); (Choudhary et al, 2011), alpha-amylase and alpha glucosidase inhibitors (Rajan, 2017), antidiabetic and antioxidant (Abusufyan et al, 2018)…”

Section: Identified Compoundsmentioning

confidence: 99%

Inhibitory activity of endophytic fungi against alpha-amylase isolated from raru (Cotylelobium melanoxylon)

Siregar¹,

Pujiyanto

Lunggani

2022

Berkala Penelitian Hayati

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Diabetes mellitus is a metabolic disorder characterized by elevated blood glucose levels. Alpha-amylase inhibitors are chemicals that prevent amylase from degrading carbohydrates into monosaccharides. For instance, the Raru plant (Cotylelobium melanoxylon), which was widely used by the local people, particularly in Sumatra, was used to lower blood glucose levels. The goal of this study was to isolate an endophytic fungus from the Raru plant and test its alpha-amylase activity by optimizing treatment time, substrate concentration, and alpha-amylase inhibitor compound extraction. The activity of alpha-amylase was measured using spectrophotometry, and starch was used as the substrate. Four isolates of the endophytic fungus were isolated from Raru bark, including TR1, TR2, TR3, and TR4. Of these, the isolate TR3 had the highest alpha-amylase activity, reaching 88.71%. Alpha-amylase was optimally inhibited for 7 days with a percentage of 90.13%. At a substrate concentration of 2%, the inhibition of alpha-amylase was observed at 96.34%. While the highest extraction of alpha-amylase inhibitory compound was observed at 0.13 ppm with a percentage of 74.71% inhibition. Based on GC-MS (gas chromatography mass spectrometry) analysis, compound 24, identified as1,3-benzenedicarboxylic acid, bis (2-ethylhexyl) ester, isophthalic acid group, was produced by endophytic fungi, exerting alpha-amylase inhibitory activity. This research would provide a new source of chemical agents to combat diabetes.

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“…The Penicillium TR3 strain displayed significant α-amylase inhibitory activity with an inhibition rate of 96.34%. They separated and identified several compounds, and compound 27 (oleic acid; Ahmad et al, 2012 ; Indrianingsih et al, 2018 ), compound 28 (1,3-benzenedicarboxylic acid, bis (2-ethylhexyl) ester, isophthalic acid group; Rajan and Aanandhi, 2017 ; Abusufyan et al, 2018 ), compound 29 (hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester (CAS), palmitin group; Venkatramanan et al, 2020 ) showed inhibitory activities against α-glucosidase and α-amylase ( Siregar et al, 2022 ).…”

Section: Inhibitors Of Glycosidases From Different Microbesmentioning

confidence: 99%

Metabolites extracted from microorganisms as potential inhibitors of glycosidases (α-glucosidase and α-amylase): A review

Wang

Shang²,

Bai³

et al. 2022

Front. Microbiol.

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α-Glucosidase and α-amylase are the two main glycosidases that participate in the metabolism of carbohydrates. Inhibitors of these two enzymes are considered an important medical treatment for carbohydrate uptake disorders, such as diabetes and obesity. Microbes are an important source of constituents that have the potential to inhibit glycosidases and can be used as sources of new drugs and dietary supplements. For example, the α-glucosidase inhibitor acarbose, isolated from Actinoplanes sp., has played an important role in adequately controlling type 2 diabetes, but this class of marketed drugs has many drawbacks, such as poor compliance with treatment and expense. This demonstrates the need for new microorganism-derived resources, as well as novel classes of drugs with better compliance, socioeconomic benefits, and safety. This review introduces the literature on microbial sources of α-glucosidase and α-amylase inhibitors, with a focus on endophytes and marine microorganisms, over the most recent 5 years. This paper also reviews the application of glycosidase inhibitors as drugs and dietary supplements. These studies will contribute to the future development of new microorganism-derived glycosidase inhibitors.

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Comparative in vitro Antidiabetic and Antioxidant Activity of Various Extracts of Ficus Species

Cited by 11 publications

References 7 publications

Methanol-Extract/Fractions of Dacryodes edulis Leaves Ameliorate Hyperglycemia and Associated Oxidative Stress in Streptozotocin-Induced Diabetic Wistar Rats

Methanol-Extract/Fractions of Dacryodes edulis Leaves Ameliorate Hyperglycemia and Associated Oxidative Stress in Streptozotocin-Induced Diabetic Wistar Rats

Inhibitory activity of endophytic fungi against alpha-amylase isolated from raru (Cotylelobium melanoxylon)

Metabolites extracted from microorganisms as potential inhibitors of glycosidases (α-glucosidase and α-amylase): A review

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