Comparative pharmacokinetics of seven bioactive components in normal, sham‐operated, and myocardial ischemia–reperfusion injury rats after oral administration of the Salvia Miltiorrhiza–Moutan Cortex herb pair

Abstract

Recently the Salvia Miltiorrhiza–Moutan Cortex (SM‐MC) herb pair is considered as a promising Chinese medicinal mixture exhibiting a range of pharmacological activities, including treating cardiovascular disease due to its unique composition. In this study, we conducted the comparative pharmacokinetic analysis of seven main bioactive components of SM‐MC in a different model rat. A straightforward ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) strategy that could simultaneously evaluate the levels of seven compounds was used to ensure the reliability of these pharmacokinetic analyses in rat plasma. The rat plasma samples were collected from normal, sham‐operated, and myocardial ischemia–reperfusion injury (MIRI) groups at predetermined time points after the administration of SM‐MC. The main pharmacokinetic parameters were detected and calculated. We successfully assessed the maximum concentration (Cmax), time to Cmax (Tmax), the elimination rate constant (λz), total half‐life (t1/2), total body clearance (CL), and the area under the concentration–time curve from 0 to last sampling time (AUC0–t) and extrapolated to infinity (AUC0–∞). To sum up, an optimized UPLC‐MS/MS approach that could be used to rapidly, simultaneously, and sensitively detect seven bioactive compounds derived from SM‐MC extract preparations was successfully developed, which may offer a pharmacokinetic basis for preclinical and clinical studies of SM‐MC herb pair for treating MIRI.

Comparative pharmacokinetic study of six lignans in normal and diabetic rats after oral administration ofSaururus chinensisextract by LC–MS/MS

“…Each rat was orally administered a single dose of 0.2 g kg À1 SC extract, suspended in 0.5% carboxymethyl cellulose sodium (CMC-Na) aqueous solution (which is equal to 2.84 g crude herbs kg À1 ). The doses were selected by converting human clinical doses (15-30 g) in Chinese Pharmacopoeia (2020 Edition, Volume I) to corresponding animal equivalents based on FDA guidelines (Yang et al, 2021). The blood samples ($0.5 mL) from the suborbital vein of each rat were collected in a 1.5 mL heparinized centrifuge tube before drug administration and at different time points of 0.083, 0.16, 0.25, 0.5, 1, 2, 4, 8, 12, and 24 h after oral administration.…”

“…It is essential for the full-scale investigation of the pharmacokinetic properties of the bioactive components in SC, because of its special significance in the evaluation of therapeutic effect, dose adjustment, and the rational use in clinic (Dong et al, 2021;Yang et al, 2021). However, to the best of our knowledge, there were fewer reports about the quantification and pharmacokinetic study of SC lignans in rat plasma, except the determination of manassantin B in rat plasma using HPLC with fluorescence detection (Lee et al, 2016) and our previously developed liquid chromatography with tandem mass spectrometric detection (LC-MS) method for the quantitative analysis of sauchinone in rat plasma (Xu et al, 2012).…”

Determination of fingerprints contents of different extracts and parts of six endemic Salvia taxa by GC–MS: Source species for valuable compounds with drug or drug potential

“…Salvia species have also been consumed as a tea and spice since ancient times. Their anti‐inflammatory, antiviral, anticholinesterase enzyme, cytotoxic and hepatotoxic activities stem from the diterpenes and triterpenes that they carry, as well as phenolic acid and flavones (Salinas et al, 2020; Topcu et al, 2007, 2017; Yang et al, 2021; Yener, 2020; Yener et al, 2020).…”