Comparative proteomic analysis of two wasps venom, Vespa tropica and Vespa affinis

Rungsa, Prapenpuksiri; Incamnoi, Paroonkorn; Sukprasert, Sophida; Uawonggul, Nunthawun; Klaynongsruang, Sompong; Daduang, Jureerut; Patramanon, Rina; Roytrakul, Sittiruk; Daduang, Sakda

doi:10.1016/j.toxicon.2016.06.005

Cited by 20 publications

(27 citation statements)

References 44 publications

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“…The wasps were collected from Siang Sao Village, Sri Songkram district, Nakorn Panom Province, northeastern Thailand [ 26 ]. The worker wasps were immediately shocked on ice.…”

Section: Methodsmentioning

confidence: 99%

“…RT-PCR was performed using the RevertAid First strand cDNA synthesis kit (Thermo Scientific, USA) as described in the instruction manual. PCR primers for the amplification of VesT2 were designed based on the sequence similarity of the conserved region of HAase from vespid venom and conserved nucleotide sequences corresponding to peptide sequences obtained from LC-MS/MS analysis (Table 1 ) [ 26 ]. The PCR was performed using green master mix reagent kits with Taq DNA polymerase (Promega, Singapore).…”

Section: Methodsmentioning

confidence: 99%

“…The greater banded wasp ( Vespa tropica ) is mostly distributed in the forest throughout Indochina peninsula including Thailand. It has a body length of up to 5 cm and its nest is usually found underground [ 26 ]. V. tropica is among the most venomous known insects.…”

Section: Introductionmentioning

confidence: 99%

“…V. tropica is among the most venomous known insects. The lethal dose of its pure venom in experimental animals (LD 50 of approximately 2.8 mg/kg in mice) is more potent than that of V. affinis venom [ 26 , 27 ]. The potency of V. tropica venom has been reported to nearly stop the end plate potentials of Drosophila larvae in nerve-muscle preparation in response to treatment with this venom [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…The potency of V. tropica venom has been reported to nearly stop the end plate potentials of Drosophila larvae in nerve-muscle preparation in response to treatment with this venom [ 28 ]. HAase was reported to be a major protein in V. tropica venom, where it is found by 2.5-fold the proportion observed in V. affinis venom [ 26 ]. The understanding of HAase in terms of biochemical and structural characterization of these wasps is important for the development of new tools for treating multiple stings and for diagnosis and therapy of allergic reactions caused by this venom.…”

Section: Introductionmentioning

confidence: 99%

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Cloning, structural modelling and characterization of VesT2s, a wasp venom hyaluronidase (HAase) from Vespa tropica

Rungsa

Incamnoi

Sukprasert

et al. 2016

J Venom Anim Toxins Incl Trop Dis

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BackgroundWasp venom is a complex mixture containing proteins, enzymes and small molecules, including some of the most dangerous allergens. The greater banded wasp (Vespa tropica) is well-known for its lethal venom, whose one of the major components is a hyaluronidase (HAase). It is believed that the high protein proportion and activity of this enzyme is responsible for the venom potency.MethodsIn the present study, cDNA cloning, sequencing and 3D-structure of Vespa tropica venom HAase were described. Anti-native HAase antibody was used for neutralization assay.ResultsTwo isoforms, VesT2a and VesT2b, were classified as members of the glycosidase hydrolase 56 family with high similarity (42–97 %) to the allergen venom HAase. VesT2a gene contained 1486 nucleotide residues encoding 357 amino acids whereas the VesT2b isoform consisted of 1411 residues encoding 356 amino acids. The mature VesT2a and VesT2b are similar in mass and pI after prediction. They are 39119.73 Da/pI 8.91 and 39571.5 Da/pI 9.38, respectively. Two catalytic residues in VesT2a, Asp107 and Glu109 were substituted in VesT2b by Asn, thus impeding enzymatic activity. The 3D-structure of the VesT2s isoform consisted of a central core (α/β)7 barrel and two disulfide bridges. The five putative glycosylation sites (Asn79, Asn99, Asn127, Asn187 and Asn325) of VesT2a and the three glycosylation sites (Asn1, Asn66 and Asn81) in VesT2b were predicted. An allergenic property significantly depends on the number of putative N-glycosylation sites. The anti-native HAase serum specifically recognized to venom HAase was able to neutralize toxicity of V. tropica venom. The ratio of venom antiserum was 1:12.ConclusionsThe wasp venom allergy is known to cause life-threatening and fatal IgE-mediated anaphylactic reactions in allergic individuals. Structural analysis was a helpful tool for prediction of allergenic properties including their cross reactivity among the vespid HAase.

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“…The wasps were collected from Siang Sao Village, Sri Songkram district, Nakorn Panom Province, northeastern Thailand [ 26 ]. The worker wasps were immediately shocked on ice.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%