Comparative Proteomics of Methicillin-Resistant <i>Staphylococcus aureus</i> Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin

Alves, Fernanda; Albano, Mariana; Andrade, Bruna Fernanda Murbach Teles; Chechi, Jéssica Luana; Pereira, Ana Flávia Marques; Furlanetto, Alessandra; Rall, Vera Lúcia Mores; Fernandes, Ana Angélica Henrique; Santos, Lucilene Delazari dos; Barbosa, Lidiane Nunes; Júnior, Ary Fernandes

doi:10.1089/mdr.2019.0038

Cited by 17 publications

(10 citation statements)

References 42 publications

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“…Due to its broad-spectrum activity, nisin A is widely used as a food additive worldwide for the prevention of food poisoning [26,32,33]. In addition, Alves DCB et al reported the potential use of nisin combined with oxacillin for methicillin-resistant S. aureus [34]. Bacteriocins, including nisin A, were also investigated as potential antibacterial chemotherapeutic agents for clinical application [26,29,35].…”

Section: Bacteriocinsmentioning

confidence: 99%

Antibacterial Peptides Resistance in Staphylococcus aureus: Various Mechanisms and the Association with Pathogenicity

Kawada‐Matsuo

Komatsuzawa

2021

Genes

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Staphylococcus aureus is a bacterium that mainly colonizes the nasal cavity and skin. To colonize the host, it is necessary for S. aureus to resist many antibacterial factors derived from human and commensal bacteria. Among them are the bacteria-derived antimicrobial peptides (AMPs) called bacteriocins. It was reported that some two-component systems (TCSs), which are signal transduction systems specific to bacteria, are involved in the resistance to several bacteriocins in S. aureus. However, the TCS-mediated resistance is limited to relatively low concentrations of bacteriocins, while high concentrations of bacteriocins still exhibit antibacterial activity against S. aureus. To determine whether we could obtain highly bacteriocin-resistant mutants, we tried to isolate highly nisin A-resistant mutants by exposing the cells to sub-minimum inhibitory concentrations (MICs) of nisin A. Nisin A is one of the bacteriocins produced by Lactococcus lactis and is utilized as a food preservative worldwide. Finally, we obtained highly nisin A-resistant mutants with mutations in one TCS, BraRS, and in PmtR, which is involved in the expression of pmtABCD. Notably, some highly resistant strains also showed increased pathogenicity. Based on our findings, this review provides up-to-date information on the role of TCSs in the susceptibility to antibacterial peptides. Additionally, the mechanism for high antimicrobial peptides resistance and its association with pathogenicity in S. aureus is elucidated.

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Section: Bacteriocinsmentioning

confidence: 99%

Antibacterial Peptides Resistance in Staphylococcus aureus: Various Mechanisms and the Association with Pathogenicity

Kawada‐Matsuo

Komatsuzawa

2021

Genes

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“…On the other hand, the mecA gene was still detected in MRSA that became oxacillin-sensitive upon exposure to nisin, indicating that gene expression could probably be affected. Alves et al, (2020) showed that MRSA strains treated with nisin and/or oxacillin showed altered protein expression compared to the control treatment, including resistance and extress response genes. Lee et al (2013) also tested the inhibitory activity of bacteriocin KU24 against MRSA and verified that the bacteriocin impaired the mecA expression.…”

Section: Discussionmentioning

confidence: 99%

Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition

Silva

Albuquerque

Oliveira

et al. 2021

RSD

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The objective of this study was to analyze the antimicrobial effect of nisin against MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus), and at the same time examine the possibility of the bacteria to develope nisin resistance. The antimicrobial susceptibility of the strains was tested using the agar diffusion and/or microdilution methods. To select nisin-resistant strains, bacteria were grown consecutively at sublethal concentrations of the bacteriocin. Nisin showed bactericidal activity against most of the tested strains. MRSA required higher doses of bacteriocin compared to MSSA both for inhibition and cell death. However, transfers in the presence of nisin could completely eliminate nisin activity with an increase in minimal inhibitory concentration value of up to 250 times. Nisin-resistance could be maintained in MRSA and MSSA even in the absence of the bacteriocin. Nisin resistance affected antibiotic susceptibility of both MRSA and MSSA to mainly Cefoxitin, Oxacillin, and Erythromycin. These results indicate that nisin-resistance is a complex trait among MSSA and MRSA and must be elucidated before the therapeutic recommendation of nisin to treat infections caused by these bacterial species.

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“…Possibly polymyxin might facilitate the transfer of nisin to its target. Along with nisin's synergistic action with polymyxin and clarithromycin against P. aeruginosa and other non-β-lactam antibiotics against MRSA [38] and strains of vancomycin-resistant enterococci [39] was also reported. Webber et al [40] embedded 0.89 µg cm −2 , positively charged nisin Z within polyelectrolyte multilayers (PEMs) i.e., 9 layers of carrageenan (CAR) and chitosan (CS), forming a 4.5 bilayer film with antimicrobial activity against S. aureus and MRSA.…”

Section: Ribosomally Synthesized Antimicrobial Peptides (Bacteriocins) and Bacteriocin-like Inhibitory Substances (Blis)mentioning

confidence: 93%

A Review on Microorganisms-Derived Products as Potential Antimicrobial Agents

Rani¹,

Saini²,

Bast³

et al. 2021

Preprint

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Microorganisms including actinomycetes, archaea, bacteria, fungi, yeast, and micro algae are the auspicious source of vital bioactive compounds. In this review, the existing state of the art re-garding antimicrobial molecules from microorganisms has been summarized. The potential an-timicrobial compounds from actinomycetes, particularly Streptomyces sp.; archaea; fungi including endophytic and marine-derived fungi, mushroom; yeast, and microalgae were briefly described. Furthermore, this review briefly summarized the activity and mode of action of bacteriocins, a ribosomally synthesized antimicrobial peptides product of Eurotium sp., Streptomyces parvulus, S. thermophiles, Lactococcus lactis, etc. Bacteriocins have inherent properties such as targeting multi-ple-drug resistant pathogens, which allows them to be considered next-generation antibiotics. Similarly, Glarea lozoyensis derived antifungal lipohexpeptides i.e., pneumocandins, inhibits 1,3-β-glucan synthase of the fungal cell wall and acts as a precursor for the synthesis of caspo-fungin, is also elaborated. In conclusion, this review highlights the possibility of using microor-ganisms as an antimicrobial resource for biotechnological, nutraceutical, and pharmaceutical ap-plications. However, more investigations are still required to separate, purify, and characterize these bioactive compounds and transfer these primary drugs into clinically approved antibiotics.

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Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin

Cited by 17 publications

References 42 publications

Antibacterial Peptides Resistance in Staphylococcus aureus: Various Mechanisms and the Association with Pathogenicity

Antibacterial Peptides Resistance in Staphylococcus aureus: Various Mechanisms and the Association with Pathogenicity

Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition

A Review on Microorganisms-Derived Products as Potential Antimicrobial Agents

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