Comparative Proteomics of Ovarian Epithelial Tumors

An, Hee Jung; Kim, Dong Su; Park, Yong Kyu; Kim, Sei Kwang; Choi, Yoon Young; Kang, Suki; Ding, Boxiao; Cho, Nam Hoon

doi:10.1021/pr050461p

Cited by 42 publications

(27 citation statements)

References 28 publications

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“…Despite the relatively small number of tissue and ascites specimens studied, the majority of proteins identified in the present study as being significantly differentially secreted from malignant and CTRL cell samples had been detected in previous studies (8)(9)(10)(11)(12)(13)(14)(15)(16), confirming their potent and important role. To the best of our knowledge, proteins including HSP-10 and DKK3, had not previously been identified by a proteomic approach, but had been suggested as potent markers using alternate approaches (19,30); however the present study was unable to confirm their utility as biomarkers of serous ovarian cancer by the immunological method.…”

Section: Discussionsupporting

confidence: 77%

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Comparative secretome of ovarian serous carcinoma: Gelsolin in the spotlight

et al. 2017

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Abstract. Ovarian cancer is one of the most common types of reproductive cancer, and has the highest mortality rate amongst gynecological cancer subtypes. The majority of ovarian cancers are diagnosed at an advanced stage, resulting in a five-year survival rate of ~30%. Early diagnosis of ovarian cancer has improved the five-year survival rate to ≥90%, thus the current imperative requirement is to identify biomarkers that would allow the early detection, diagnosis and monitoring of the progression of the disease, or of novel targets for therapy. In the present study, secreted proteins from purified ovarian control, benign and cancer cells were investigated by mass spectrometry, in order to identify novel specific markers that are easy to quantify in patients sera. A total of nine proteins revealed significant differential secretion from control and benign cells, in comparison with ovarian cancer cells. The mRNA expression levels of three of these proteins (Dickkopf protein 3, heat shock protein 10 kDa and gelsolin) were subsequently evaluated by reverse transcription-quantitative polymerase chain reaction. Combined with the protein level in serum, the present study identified that gelsolin may be a useful marker of ovarian cancer.

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Section: Discussionsupporting

confidence: 77%

“…Certain previous studies have analyzed the proteomic profiles of ovarian tumor tissues, cell lines, urine, ascites fluid and blood samples (8)(9)(10)(11)(12)(13)(14). Analysis of the ovarian cancer cell line secretome has also provided information on potential markers (11,15).…”

Section: Introductionmentioning

confidence: 99%

Comparative secretome of ovarian serous carcinoma: Gelsolin in the spotlight

et al. 2017

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“…These candidate genes included nm23, annexin I, protein phosphatase-1, ferritin, proteasome a-6, N-acetyl glucosamine kinase (NAGK) and an A6-related protein. 4 Interestingly, we found that the nm23/NDP kinase family is typically overexpressed in ovarian cancers but generally underexpressed in metastatic cells. In the current study, we use proteomics and immunohistochemistry to examine the biological role of nm23 in serous ovarian carcinomas.…”

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confidence: 79%

“…3 We have previously used a comparative proteomic approach and various other subtractive and comparative methods to examine the gene expression profiles of ovarian carcinomas. 4 The results of these studies have led to the identification of several putative biomarkers for ovarian carcinomas. These candidate genes included nm23, annexin I, protein phosphatase-1, ferritin, proteasome a-6, N-acetyl glucosamine kinase (NAGK) and an A6-related protein.…”

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confidence: 99%

NM23 as a prognostic biomarker in ovarian serous carcinoma

Youn

Kim²,

Kim

et al. 2008

Modern Pathology

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The nm23 gene is a reported metastasis suppressor gene. Recent studies have shown that its expression has tissue specificity. The role of nm23 in human ovarian cancer is still controversial. This study examines the prognostic significance of nm23 expression in patients with serous ovarian carcinoma. Following comparative proteomics in 13 fresh frozen ovarian serous cancer tissues with other histological types of ovarian cancers, validation was performed using immunohistochemistry on clinically well-designed 73 ovarian serous carcinoma microarray samples that were retrieved from ovarian cancer patients from 1990 to 2003. Statistical analysis of the results was performed using v 2 test, Cox proportional regression, the Kaplan-Meier method and log-rank test. We found that the expression of nm23 inversely correlated with peritoneal seeding (P ¼ 0.009). However, strong nm23 expression was associated with mortality in patients with ovarian carcinoma in univariate analysis (P ¼ 0.04). Poor prognostic factors of disease-free survival included tumor residue more than 2 cm (P ¼ 0.02), bilaterality (P ¼ 0.01) and peritoneal seeding (Po0.01), whereas poor prognostic factors affecting overall survival included peritoneal seeding (P ¼ 0.05). In Kaplan-Meier analysis, strong nm23 immunoreactivity correlates with poor overall survival (P ¼ 0.04) but not with poor disease-free survival. In conclusion, overexpression of nm23 is independently associated with decreased overall survival in patients with ovarian carcinoma and also significantly correlates with mortality. Nm23 may have a biological function that leads to poor clinical outcomes in ovarian carcinoma.

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“…Epithelial proteomics has the potential to make a major contribution to our understanding of the pathogenesis of organ dysfunction when combined with clinical measures, tissue imaging and profiling, and organ dysfunction score systems [6]. Epithelial proteomic profiles have been investigated in multiple pathophysiological conditions, including cancer, inflammation, stress, infection, and others [7][8][9][10]. BBMs are considered the first line of defense of epithelial cells against inhaled external elements, and it is known that interaction with such stimuli can trigger intracellular signals.…”

Section: Introductionmentioning

confidence: 99%

Epithelial Brush Border Proteomics and Associated Cell Dysfunction

Wang¹,

Adler²

2008

JEBP

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Functions and structures of epithelial cells vary, depending upon their locations in organs/tissues. Pathophysiological responses of epithelia are associated with challenges, severities, durations and diseases. Epithelial proteomics has been used to understand the role of these cells in the pathogenesis of several diseases, and has the potential to identify novel biomarkers for disease prognosis, diagnosis and therapies. Epithelial brush border membranes (BBMs) are involved in digestion, absorption, metabolism, and transport of nutrients and drugs, clearance of and defense against toxins, and initiation of intracellular signaling and maintenance of cellular structural integrity. The present article review proteomic research on epithelial brush borders to understand the potential links among the function and disease associations of epithelial BBM proteomics. We found that the amount and functional ratio of epithelial cell BBM proteomic profiles obviously vary among species, cell locations, organ functions, research groups, and methodologies utilized. Many of these proteins may be identified and their functions elucidated, probably providing potential new markers for epithelial-associated diseases and potential therapeutic targets. There is still a great need for proteomic studies for analysis and comparisons of BBM protein profiles associated with normal physiological and pathophysiological states, organ function and dysfunction, and clinical health and disease.

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Comparative Proteomics of Ovarian Epithelial Tumors

Cited by 42 publications

References 28 publications

Comparative secretome of ovarian serous carcinoma: Gelsolin in the spotlight

Comparative secretome of ovarian serous carcinoma: Gelsolin in the spotlight

NM23 as a prognostic biomarker in ovarian serous carcinoma

Epithelial Brush Border Proteomics and Associated Cell Dysfunction

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