Comparative Study on Bactericidal Effect of Silver Nanoparticles, Synthesized Using Green Technology, in Combination with Antibiotics on Salmonella Typhi

Abstract

In this work bactericidal study of silver nanoparticles was taken up in combination with two standard antibiotics, ampicillin and gentamycin, for Salmonella typhi. The antibacterial activities of antibiotics were increased in the presence of AgNPs against test strains. The higher enhancing effect was observed for ampicillin in comparison to gentamicin against test strains. Silver nanoparticles were synthesized elctrolytically using silver wire of 99% purity as anode and carbon rod wrapped with LDPE as cathode. Silver nitrate [of Merck] of 0.01N is used as an electrolyte. Here tea extract is added as capping and mild reducing agent. The polyphenols theaflavins and thearubigins, present in tea perform the role of stabilizing or capping agents due to their bulky and steric nature. A brown coloured colloidal solution of silver nanoparticles is obtained. The as-synthesized silver nanoparticles were characterized using XRD, TEM and UV-Vis spectroscopy.

Synthesis and Antibacterial Activity of CuO Nanoparticles Suspension Induced by Laser Ablation in Liquid

“…The antibiotic molecules contain many active groups, such as hydroxyl and amino groups, which react easily with NPs by chelation. Thus, the synergistic effect may be due to the bonding reaction with antibiotic and NPs [26]. Another mechanism added by Tiwari and his colleagues, is the fact that the phospholipids and glycopro- Where A is representing amoxicillin only, and 1, 2, 3 are representing CuO NPs (plus amoxicillin) with concentrations 400, 600, and 1000 µg mL −1 , respectively tein present in the bacteria cell membrane are hydrophobic groups.…”

Electrochemical synthesis, characterisation and phytogenic properties of silver nanoparticles

“…For the past few decades, nanoparticles of silver have been gaining much attention and being used in almost every field, including medicine (Nithya and Ragunathan 2014;Knetsch and Koole 2011), catalysis (Venkatesham et al 2012), bio sensing (Priyanka et al 2013;Doria et al 2012;Zhu et al 2009), drug delivery (Soumya and Gayatri Hela 2013;Prabhu and Uzzaman 2011;Diaz and VivasMejia 2013), electronics Lu 2007;Karni et al 2012), textile (Xue et al 2012), photonics (Shen et al 2000), optical sensor (Pandey et al 2012), nonlinear optical properties (Ye et al 2009;Vasireddy and Paul 2012), water treatment (Monyatsi et al 2012;Ebeling et al 2013), pigments, photographic, bactericide (Kim et al 2007;Govarthanan et al 2014;Rajawat and Qureshi 2012;Li et al 2013;Yasina et al 2013), anticancer agent (Devi and Bhimba 2012;Mukherjee et al 2014;Sulaiman et al 2013;Nazir et al 2011), wound treatment (Habiboallah et al 2014, conductive composites (Pandey et al 2012), etc.…”

Comparative Study of Synergistic Effects of Antibiotics with Triangular Shaped Silver Nanoparticles, Synthesized Using UV-Light Irradiation, on <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>

“…Hence there is a need to find ways to formulate green and less toxic materials. Plant extracts play an important role in remediation of toxic metals through reduction of the metal ions [18]. In the present synthesis route, the author has developed a green route by using tea leaf extract as capping agent, UV-irradiation source as a reducing agent and chitin flakes as stabilizing agent.…”

“…Compounds of silver like silver oxalate (Ag 2 C 2 O 4 ) are photosensitive and yield metallic silver upon the exposure to UV light in the presence of capping agents. Studies [17] [18] show that antimicrobial formulations of silver in the form of nanoparticles could be used as effective bactericidal materials. In the present work, synergistic effect of silver nanoparticles is studied in combination with two standard antibiotics against gram positive and gram negative bacteria.…”