Synthesis and Antibacterial Activity of CuO Nanoparticles Suspension Induced by Laser Ablation in Liquid

Abstract

In the present study, copper oxide (CuO) colloidal nanoparticles (NPs) were synthesized using laser ablation of copper pellet immersed in deionized water. Pulsed Nd: YAG laser was used to irradiate the targets at different laser energies and various ablation times. NP suspensions were characterized by UV-Vis spectroscopy, XRD, and TEM. The absorption spectrum exhibited a peak at ∼275 nm and another peak with low intensity at ∼645 nm. The XRD pattern of the NPs proved the presence of (-111) and (112) planes assigned to the CuO phase. The TEM images showed nearly spherical shape nanoparticles CuO NPs with size of 3-40 nm. The antibacterial activity of CuO NPs was first carried out against four types of bacteria: Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris and Staphylococcus aureus, by liquid medium method. CuO NPs showed the highest antibacterial activity against E. coli at the highest concentration (1000 µg mL −1 ). CuO NPs and amoxicillin had a synergistic effect on inhibiting E. coli and S. aureus growth; this effect was also tested using the well diffusion method. In this method, CuO NPs at a concentration of 1000 µg mL −1 along with amoxicillin showed the inhibition zone against E. coli (26.0 ± 1.00 mm), as well as complete inhibition of bacteria against S. aureus.

Antibacterial Behavior of Laser-Ablated Copper Nanoparticles

“…This is due to the fact that Cu NPs fabricated by LASiS method are in pure and colloidal form and the particle size is smaller as compared to nanoparticles produced by chemical reduction method. Khashan et al [31] measured the zone of inhibition of CuO with amoxicillin for E. coli ((26.0 ± 1.00) mm) with concentration of 1000 lg/ml. Ramyadevi et al [32] calculated zone of inhibition of S. aureus (21 mm) and E. coli (26 mm).…”

Antibacterial Activity of Copper Oxide (CuO) Nanoparticles Biosynthesized by Bacillus sp. FU4: Optimization of Experiment Design

“…Results of this study demonstrated that E. coli was more sensitive than other bacteria species at a highest concentration of CuO NPs (1000 μg mL) with a inhibition zone of 26.0 ± 1.00 mm. 62 Sonia and coworkers (2016) investigated the antibacterial activity of CuO NPs in three different concentrations (12.5 μg/ml, 25 μg/ml and 50 μg/ml) by agar diffusion method against four pathogenic bacteria species: Serratia marcescens, Streptococcus pneumonia, Staphylococcus aureus, and Salmonella typhimurium. 61 The minimum inhibitory concentration (MIC) for all nanostructures was in the range of 12.5 μg/ml to 50 μg/ml.…”

Optimization Properties and Characterization of Green Synthesis of Copper Oxide Nanoparticles Using Aqueous Extract of Cordia myxa L. Leaves

“…Effect of pH: Green synthesis of copper oxide nanoparticles using aqueous extract of Cordia myxa L. leaves was examined over a broad pH range (3)(4)(5)(6)(7)(8)(9)(10)(11). Variations in pH were highly affected the SPR of CuONPs.…”

“…Their characters depended on certain trait such as size particle, distribution, morphology [4] and high surface/volume ratio [5]. Metal oxide nanoparticles obtained great attention for their potential applications in optoelectronics, nanodevices, nanoelectronics, nanosensors, information storage and catalysis [6,7].…”