Comparative transcriptome analysis reveals significant differences in gene expression between appressoria and hyphae in Colletotrichum gloeosporioides

Zhou, Zhongshi; Wu, Jianyuan; Ji, Zhirui; Zhang, Junxiang

doi:10.1016/j.gene.2018.05.080

Cited by 21 publications

(17 citation statements)

References 55 publications

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“…It is reported that deletion of MAPK gene CMK1 in C. lagenarium prevents germination, whereas deletion of MAPK gene pmk1 in Magnaporthe grisea, blocks appressorium development but not germination (Osherov and May, 2001). The MAPK pathway was shown to be essential for appressorium development in C. gloeosporioides (Wang et al, 2018). However, in our case, GT formation was not inhibited in the presence of MAPK pathway inhibitor Insolution™ PD98059, and moreover, no transcripts related to MAPK pathway could be detected in GT transcriptome, thereby indicating that MAPK pathway may not be essential for GT formation in C. gloeosporioides.…”

Section: Transcriptome Analysis Of Gtcontrasting

confidence: 64%

“…Fungal transporter proteins play a broad range of biological functions, and an important role in pathogenicity of phytopathogens (Gupta and Chattoo, 2008;Stefanato et al, 2009;Sun et al, 2006). In previous studies, it has been demonstrated that an ABC protein CgABCF2 was required for appressorium formation and plant infection in C. gloeosporioides (Wang et al, 2018;Zhou et al, 2017). However, in the present study, few transporters and signalling genes viz.…”

Section: Transcriptome Analysis Of Gtcontrasting

confidence: 54%

“…1F), thereby suggesting that this pathway may not be a significant player in early GT formation. However, the very same pathway was shown to be essential in appressorium formation (Wang et al, 2018).…”

Section: Differential Physiological Requirements For Gt and Catmentioning

confidence: 99%

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Deciphering the differential physiological and molecular requirements for conidial anastomosis tube fusion and germ tube formation inColletotrichum gloeosporioides

Mehta

Patil

Baghela

2021

Preprint

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The conidia of a hemibiotrophic fungus Colletotrichum gloeosporioides can conventionally form germ tube (GT) and develop in to a fungal colony, while under certain conditions, they tend to get connected with each other through conidial anastomosis tube (CAT) so as to share the nutrients. CAT fusion is believed to be responsible for generation of genetic variations in few asexual fungi, which appears problematic for effective fungal disease management. The physiological and molecular mechanism underlying the GT versus CAT formation remained unexplored. In the present study, we have deciphered the decision switch responsible for GT formation versus CAT fusion in C. gloeosporioides. GT formation occurred at high frequency in the presence of nutrients, while CAT fusion was found to be higher in absence of nutrients. Younger conidia were found to form GT efficiently, whilst older conidia preferentially formed CAT. Whole transcriptome analysis of GT and CAT fusion revealed differential molecular requirements for these two processes. We identified 11050 and 9786 differentially expressed genes (DEGs) in GT and CAT, respectively. A total 1567 effector candidates were identified, of them 103 and 101 were uniquely secreted during GT and CAT formation respectively. Genes coding for cell wall degrading enzymes, germination, hyphal growth, host-fungus interaction and virulence were up-regulated during GT formation. Whilst, genes involved in stress response, cell wall remodelling, membrane transport, cytoskeleton, cell cycle, and cell rescue were highly up-regulated during CAT fusion. To conclude, the GT and CAT fusion were found to be mutually exclusive processes, requiring differential physiological conditions and sets of DEGs in C. gloeosporioides. This will help to understand the basic CAT biology in the genus Colletotrichum.

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Section: Transcriptome Analysis Of Gtcontrasting

confidence: 64%

Section: Transcriptome Analysis Of Gtcontrasting

confidence: 54%

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Deciphering the differential physiological and molecular requirements for conidial anastomosis tube fusion and germ tube formation inColletotrichum gloeosporioides

Mehta

Patil

Baghela

2021

Preprint

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“…The Pa11 protein is associated with the maintenance of cylindrical cellular morphology and was previously shown to be involved in cellular morphogenesis and cell wall integrity in fission yeast (Ge et al, 2005). Additionally, a recent study showed that one M. robertsii Peptidase (M43) is involved in sporulation, cell wall integrity and virulence (Zhou et al, 2018). Here, the higher number of orthologous genes related to Peptidase M43 in M. rileyi (n=3) compared to one gene found in only three Metarhizium spp.…”

Section: Signature Of Yeast-like Cells In Solid Culture Mediummentioning

confidence: 79%

“…The enzyme Peptidase M43, pregnancy-associated plasma-A was showed to be involved in sporulation, cell wall integrity and virulence factors in M. robertsii (Zhou et al, 2018). Here we found that M. rileyi had three orthologous genes related to Peptidase M43 in the orthogroup OG0007192 while M. album, M. brunneum and M.…”

Section: Signature Of Yeast-like Cells In Solid Culture Mediummentioning

confidence: 99%

Advances in >i<Metarhizium>/i< blastospores production and formulation and transcriptome studies of the yeast and filamentous growth

Iwanicki¹

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Advances in Metarhizium blastospores production and formulation and transcriptome studies of the yeast and filamentous growth Biological control of pests is a growing market in the world. It is expected that the use entomopathogenic fungi to control pests will take an important share of this market. Most fungal products in the world are based on aerial conidia produced by solid fermentation using cereal grains. An alternative for aerial conidia is the use of blastospores, yeast-like hydrophilic cells that can be produced in large amounts by liquid fermentation in a short time (<4 days), in a small space and with low hand labor compared to the solid fermentation method. Therefore, the main objectives of the present studies were first to optimize a liquid culture medium for low cost production of Metarhizium blastopores; second: to assess the bioactivity of air-dried blastospores against the cattle-tick Rhipicephalus microplus; third: to develop an airdried and spray-dried Metarhizium blastospore formulation with bioactivity against the corn-leafhopper Dalbulus maidis; fourth: to improve the shelf-life of the best air-dried and spray-dried formulations stored in refrigerated (± 4°C) and in ambient conditions (28°C) using oxygen and moistures absorbrs or vacuum and fifth: to use comparative genome-wide transcriptome analyses to determine changes in gene expression between the filamentous and blastospore growth phases in vitro to characterize physiological changes and metabolic signatures associated with M. anisopliae and M. rileyi dimorphism. We showed that blastospore production of Metarhizium is isolate-and species-dependent. Glucose-enriched cultures inoculated with pre-cultures improved yields reaching optimal growth for Metarhizium robertsii ESALQ1426 (5.9 × 10 8 blastospores/mL) within 2 d. Resultant air-dried blastospores of ESALQ1426 were proved to quickly kill R. micropulus larvae with an efficiency comparable to that of conidia. We argue that both osmotic pressure, induced by high glucose titers, and isolate selection are critical to produce high yields of blastospores that hold promise to control R. microplus larvae. Fermentation experiments based on growth kinetics defined a low-cost medium using 80 g/L corn steep liquor as the most suitable nitrogen source for inducing blastospore growth in M. robertsii (4.7 × 10 8 cells/mL) in only 2 days of cultivation at a total cost of $0.30 USD per L. The dried blastospores were as high virulence as fresh cells to D. maidis adults fed on maize plants. Co-formulants were selected to compose formulations that allowed to keep M. roberstii blastospore viability after drying. The most promising resulting spray-dried and air-dried were as infective as fresh blastopores to the corn leafhopper inducing mortality rates ranging from 60.3 to 78.2% after spraying with 5 × 10 7 blastospores/mL. The LC 50 was significantly higher for spray-dried formulation (2.42 × 10 7) than for the air-dried formulation (4.65 × 10 6), suggesting a possible detrimental effect of the former tech...

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Overexpression of ZmSAG39 in maize accelerates leaf senescence in Arabidopsis thaliana

et al. 2022

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Comparative transcriptome analysis reveals significant differences in gene expression between appressoria and hyphae in Colletotrichum gloeosporioides

Cited by 21 publications

References 55 publications

Deciphering the differential physiological and molecular requirements for conidial anastomosis tube fusion and germ tube formation inColletotrichum gloeosporioides

Deciphering the differential physiological and molecular requirements for conidial anastomosis tube fusion and germ tube formation inColletotrichum gloeosporioides

Advances in >i<Metarhizium>/i< blastospores production and formulation and transcriptome studies of the yeast and filamentous growth

Overexpression of ZmSAG39 in maize accelerates leaf senescence in Arabidopsis thaliana

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