Comparison and differentiation of Wheat Yellow Mosaic Virus(WYMV), Wheat Spindle Streak Mosaic Virus (WSSMV) and Barley Yellow Mosaic Virus (BaYMV) isolates using WYMV monoclonal antibodies

Abstract

Twelve monoclonal antibodies (MAbs) were obtained by immunizing mice with a French isolate (F1) of wheat yellow mosaic virus (WYMV). Three of these (3D12, 2C1, 6C3) belong to the IgM class and the nine others to the IgG class (3D8, 3H1, 2B8, 1F2, 3C10, 4F12, 3H9, 1G5, 54). In antigen-coated plate (ACP) ELISA and indirect double antibody sandwich (IDAS) ELISA, all MAbs recognize the WYMV (F1) both in the form of purified particles and in wheat leaf extract. The analysis of numerous French isolates of WYMV shows a variable reactivity with MAbs 3D8, 3H1,2B8, 3C10, 3H9 and 1G5 in IDAS -and ACP-ELISA. The Japanese isolate of WYMV and United States isolates of wheat spindle streak mosaic virus (WSSMV) were detected in IDAS-and ACP-ELISA by ten of the MAbs tested showing that the wheat bymoviruses originating from the three locations share a high epitopic homology. French isolates of barley yellow mosaic virus (BaYMV; pathotypes 1 and 2) were only detected in ACP-EL1SA with MAbs 6C3, 3D8, 3H1 and 2B8 whereas the two Japanese strains (I-1, II-1) of MaYMV were recognized with these and also with that of 3C10. In IDAS-ELISA, the two Japanese strains were clearly detected by MAbs, 6C3, 3D8, 3H1, 1F2, 3C10 and 1G5 and the British and Belgian (pathotype 2) isolates only by that of 6C3. Only the Japanese strain of BaYMV, 1-1 could be detected with MAb 3H9 in this ELISA system.

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)

“…For this reason and the fact that several virus species exist together in the field, detection and diagnosis of WYMV is an important area of study. Several diagnostic methods are available for WYMV detection in wheat plants, including biological assay (virus inoculation and symptom expression in diagnostic plant species), ELISA (enzyme-linked immunosorbent assay) [15], immunoelectron microscopy, RT-PCR (reverse transcription polymerase chain reaction; [16] and RT-LAMP (reverse transcription loop-mediated isothermal amplification; [17] However, biological assays are time-consuming and have low levels of sensitivity and specificity. ELISA and immunoelectron microscopy are limited by the supply and quality of antiserum or the specific probe, as well as by the type of sampling.…”

Monoclonal Antibodies to Identify Tomato Mosaic Tobamovirus (Tomv)

“…They are the best ones as field test for identification of different strains, with the frequency of about 70% of the total IgG cells decreasing cross-reaction with other compounds (9). In conclusion, 8G7G2 monoclonal antibody gives the highest reaction among the four MAbs selected and is undoubtedly able to recognize ToMV with high specificity.…”

“…Examples like this have been observed elsewhere for quite similar epitopes under scrutiny as observed for detection and characterization of PPV (10) and for WSSMV viruses (9). Where monoclonal antibodies cross-reactivity is detected Western Blot analysis is used as done before to distinguish among prunus virus isolates (PVIS) from plum pox potyvirus (PPV) (10).…”

Identification of RFLP markers for resistance to wheat spindle streak mosaic bymovirus (WSSMV) disease

“…Once thought to be synonymous with wheat yellow mosaic bymovirus (WYMV), which infects wheat in Asia (Usugi and Saito 1979), WSSMV is now thought to be distinct from WYMV. These viruses are very closely related to each other and to barley yellow mosaic virus (BaYMV), and are of the genus Bymovirus in the family Potyviridae (Barnett 1991;Hariri et al 1996;Namba and Kashiwazaki 1998;Xiaoyun et al 1998). WSSMV was described on winter wheat for the first time in southern Canada in 1960 (Slykhuis 1960) and in the United States in Michigan in 1970 (Wiese et al 1970).…”